breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 720,492 T→C intergenic (‑32/‑91) speF ← / → ybfK ornithine decarboxylase, degradative/uncharacterized protein YbfK
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,294,541 C→A pseudogene (782/897 nt) insZ ← putative transposase InsZ
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,367,100 C→A S271S (TCG→TCT pspF ← DNA‑binding transcriptional dual regulator PspF
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,759,675 G→T G283G (GGC→GGA sufS ← L‑cysteine desulfurase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,992,912 C→A G599C (GGT→TGT)  uvrC ← excision nuclease subunit C
RA 2,072,994 C→A L486I (CTC→ATC)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,464,372 C→A M212I (ATG→ATT mlaA ← intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA
RA 2,481,363 G→T R272S (CGT→AGT)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,487,336 C→A A988D (GCC→GAC)  evgS → sensory histidine kinase EvgS
RA 2,751,754 T→A intergenic (+45/‑41) nadK → / → recN NAD kinase/DNA repair protein RecN
JC 3,277,273 (TTCAACA)2→3 coding (272/336 nt) prlF → antitoxin PrlF
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,359,777 C→A L194M (CTG→ATG)  gltD → glutamate synthase subunit GltD
RA 3,364,324 G→T W506C (TGG→TGT yhcD → putative fimbrial usher protein YhcD
RA 3,468,881 G→T P325T (CCA→ACA)  chiA ← endochitinase
RA 3,531,387 G→T G351G (GGC→GGA yhgE ← putative transport protein YhgE
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,579,974 C→A E278* (GAG→TAG)  yhhX ← putative oxidoreductase YhhX
RA 3,628,311 G→T L410I (CTC→ATC)  rbbA ← ribosome‑associated ATPase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,113,616 C→A V108V (GTC→GTA uspD → universal stress protein D
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196287 1211437 15151 15 [12] [13] 17 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2521007 2521331 325 47 [0] [12] 15 valX–lysV valX,valY,lysV
* * ÷ NC_000913 3102986 3104083 1098 14 [13] [13] 14 mutY mutY
* * ÷ NC_000913 3423782–3424230 3424526–3424242 13–745 14 [13] [13] 15 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 781577 =50 (0.800)46 (0.750)
+GAAG
21/268 1.5 65.5% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 0 (0.000)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 3363001 =105 (1.670)101 (1.620) 57/274 0.1 65.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)68 (1.080) 45/276 0.2 97.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA