breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 278,744 +A intergenic (‑239/+75) PP_0224 ← / ← sctC DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein
RA 307,866 +C intergenic (+881/‑988) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein
RA 336,125 +T coding (210/237 nt) PP_0278 → hypothetical protein
RA 353,067 A→G intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
RA 499,203 A→G intergenic (+47/‑249) uhpA → / → PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
RA 1,070,248 +GA intergenic (+48/+94) aroP‑I → / ← PP_0928 aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
RA 1,419,549 +C intergenic (‑38/+98) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein
RA 1,499,480 2 bp→AC intergenic (+57/‑106) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,498 1 bp→CG intergenic (+75/‑89) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,508 +C intergenic (+85/‑79) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,580,735 C→T G221D (GGC→GAC)  ttgB ← efflux pump membrane protein TtgB
RA 1,932,640 +C intergenic (‑107/+43) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
RA 2,655,897 C→T P98P (CCG→CCA cysH ← phosphoadenosine phosphosulfate reductase
RA 2,921,460 G→A G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin
RA 3,787,503 Δ2 bp coding (1131‑1132/1392 nt) PP_3350 ← hypothetical protein
RA 3,845,705 +G intergenic (‑72/+26) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
RA 3,951,159 T→C intergenic (‑123/+72) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 3,951,161 A→T intergenic (‑125/+70) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 4,586,030 2 bp→TC intergenic (+113/+101) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,035 +G intergenic (+118/+97) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,057 +C intergenic (+140/+75) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,715,625 A→T M286L (ATG→TTG)  PP_4173 → two‑component system sensor histidine kinase/response regulator
RA 4,740,811 (T)7→6 intergenic (+48/+705) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,819 2 bp→CT intergenic (+56/+696) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,229 A→C intergenic (+466/+287) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,231 C→G intergenic (+468/+285) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,234 C→T intergenic (+471/+282) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,236 2 bp→TG intergenic (+473/+279) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,239 A→G intergenic (+476/+277) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,243 3 bp→TGC intergenic (+480/+271) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,255 C→T intergenic (+492/+261) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,257 A→C intergenic (+494/+259) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,260 2 bp→GC intergenic (+497/+255) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,263 A→C intergenic (+500/+253) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,272 A→G intergenic (+509/+244) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,274 A→C intergenic (+511/+242) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,276 G→C intergenic (+513/+240) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,278 C→T intergenic (+515/+238) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,283 +T intergenic (+520/+233) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,289 C→G intergenic (+526/+227) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,292 C→T intergenic (+529/+224) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,294 C→G intergenic (+531/+222) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,296 A→C intergenic (+533/+220) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,298 C→T intergenic (+535/+218) gltA → / ← yeiW citrate synthase/putative oxidoreductase
JC 4,980,585 +GGC intergenic (‑24/+44) PP_4387 ← / ← flgE hypothetical protein/flagellar hook protein FlgE
JC 5,555,608 +GCC intergenic (‑84/+87) PP_4887 ← / ← PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 5,674,751 +G intergenic (+113/+27) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,674,753 G→C intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,948,189 G→C D95E (GAC→GAG rho ← transcription termination factor Rho
RA 6,013,885 T→C intergenic (+90/+20) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171377–172964 176293–172967 4–4917 9 [8] [7] 11 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947 176668–178394 181689–178397 4–5022 9 [8] [8] 10 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 1367592 1367848 257 9 [8] [8] 9 PP_1193 membrane protein
* * ÷ NC_002947 2299472 2299575 104 9 [8] [8] 9 sbcC exonuclease subunit SbcC
* * ÷ NC_002947 4808127 4808173 47 9 [8] [0] 11 PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* * ÷ NC_002947 5905426 5905581 156 10 [8] [8] 9 spuG spermidine/putrescine ABC transporter permease

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 2301453 =0 (0.000)28 (0.840) 26/284 0.4 100% intergenic (‑97/‑234) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 2925429 = NA (NA)intergenic (‑177/+388) PP_2563/PP_2564 antibiotic biosynthesis protein/quercetin 2,3‑dioxygenase
* ? NC_002947 = 23014540 (0.000)26 (0.770) 22/286 0.7 100% intergenic (‑98/‑233) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 5545321 = NA (NA)intergenic (‑461/+416) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NC_002947 3366553 =32 (0.930)34 (1.010) 29/286 0.3 67.6% intergenic (+219/‑142) PP_2962/PP_2964 alcohol dehydrogenase/transposase
?NC_002947 4950854 = 1 (0.030)intergenic (‑4/+153) fliL/fliK flagellar protein FliL/flagellar hook‑length control protein FliK
* ? NC_002947 = 338356543 (1.260)31 (0.910) 28/290 0.3 58.5% coding (501/546 nt) PP_2985 hypothetical protein
?NC_002947 = 4950858 1 (0.030)intergenic (‑8/+149) fliL/fliK flagellar protein FliL/flagellar hook‑length control protein FliK
* ? NC_002947 4808174 =0 (0.000)7 (0.270) 6/220 2.6 48.3% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 = 5988935 15 (0.580)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 5045678 =0 (0.000)31 (0.920) 28/284 0.3 100% intergenic (‑45/+48) PP_4449/PP_5682 hypothetical protein/hypothetical protein
?NC_002947 = 5545666 NA (NA)intergenic (‑806/+71) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NC_002947 = 50456790 (0.000)51 (1.510) 42/286 0.0 100% intergenic (‑46/+47) PP_4449/PP_5682 hypothetical protein/hypothetical protein
?NC_002947 5222582 = NA (NA)coding (8/840 nt) PP_4602 AraC family transcriptional regulator
* ? NC_002947 5545321 =NA (NA)41 (1.210) 37/286 0.1 100% intergenic (‑461/+416) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
?NC_002947 = 5621245 0 (0.000)coding (19/774 nt) PP_4939 hypothetical protein
* ? NC_002947 = 5545666NA (NA)60 (1.790) 39/284 0.0 100% intergenic (‑806/+71) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
?NC_002947 5621244 = 0 (0.000)coding (20/774 nt) PP_4939 hypothetical protein