breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000731 | 81 | N→G | 100% | intergenic (–/‑151) | – / → repA | –/replication protein A |
JC | CP000731 | 6,552 | +28 bp | 100% | intergenic (+1069/‑419) | cadX → / → USA300HOU_pUSA300HOUMR0010 | cadmium efflux regulator/MarR family transcriptional regulator |
RA | CP000731 | 25,663 | Δ1 bp | 100% | coding (316/333 nt) | USA300HOU_pUSA300HOUMR0031 → | hypothetical protein |
RA | USA300TCH1516_ALE | 11,042 | A→G | 7.7% | V41V (GTA→GTG) | hutH → | Histidine ammonia‑lyase |
RA | USA300TCH1516_ALE | 110,651 | C→T | 9.2% | D170D (GAC→GAT) | USA300HOU_RS00515 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 110,660 | T→A | 22.3% | I173I (ATT→ATA) | USA300HOU_RS00515 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 111,512 | A→G | 33.6% | S194S (TCA→TCG) | USA300HOU_RS00520 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 111,516 | C→A | 30.6% | Q196K (CAA→AAA) | USA300HOU_RS00520 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 293,940 | C→T | 25.0% | intergenic (+57/‑471) | gatD → / → tarI' | Galactitol‑1‑phosphate 5‑dehydrogenase/Ribitol‑5‑phosphate cytidylyltransferase 2 |
RA | USA300TCH1516_ALE | 297,176 | C→T | 6.1% | S337L (TCA→TTA) | tarK → | Teichoic acid ribitol‑phosphate polymerase TarK |
RA | USA300TCH1516_ALE | 478,502 | C→A | 100% | D342E (GAC→GAA) | nuoL → | NADH‑quinone oxidoreductase subunit L |
RA | USA300TCH1516_ALE | 479,096 | T→C | 5.5% | F41F (TTT→TTC) | USA300HOU_RS02280 → | hypothetical protein |
RA | USA300TCH1516_ALE | 512,643 | A→G | 44.7% | intergenic (+711/‑6052) | recR → / → speA_2 | Recombination protein RecR/Arginine decarboxylase |
RA | USA300TCH1516_ALE | 538,142 | T→C | 9.6% | F151F (TTT→TTC) | mfd → | Transcription‑repair‑coupling factor |
JC | USA300TCH1516_ALE | 567,290 | Δ3 bp | 5.5% | coding (194‑196/471 nt) | ctsR → | Transcriptional regulator CtsR |
RA | USA300TCH1516_ALE | 584,121 | G→T | 100% | intergenic (+205/‑67) | rplA → / → rplJ | 50S ribosomal protein L1/50S ribosomal protein L10 |
RA | USA300TCH1516_ALE | 656,279 | G→T | 9.3% | W71L (TGG→TTG) | USA300HOU_RS03135 → | hypothetical protein |
RA | USA300TCH1516_ALE | 707,909 | A→T | 100% | M1M (TTG→ATG) † | znuC_1 ← | High‑affinity zinc uptake system ATP‑binding protein ZnuC |
RA | USA300TCH1516_ALE | 737,162 | A→G | 7.6% | R548R (AGA→AGG) | bceB_1 → | Bacitracin export permease protein BceB |
RA | USA300TCH1516_ALE | 788,134 | A→T | 5.8% | K100N (AAA→AAT) | USA300HOU_RS03860 → | hypothetical protein |
RA | USA300TCH1516_ALE | 873,442 | A→G | 5.1% | E116G (GAA→GGA) | clfA → | Clumping factor A |
RA | USA300TCH1516_ALE | 973,213 | C→A | 100% | E175* (GAG→TAG) | yitU_1 ← | 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU |
RA | USA300TCH1516_ALE | 1,002,200 | T→G | 100% | M173R (ATG→AGG) | mecA → | Adapter protein MecA 1 |
RA | USA300TCH1516_ALE | 1,062,480 | T→C | 9.0% | E40G (GAA→GGA) | USA300HOU_RS05245 ← | Acetyltransferase |
RA | USA300TCH1516_ALE | 1,105,994 | T→C | 100% | T12T (ACT→ACC) | pdhD → | Dihydrolipoyl dehydrogenase |
RA | USA300TCH1516_ALE | 1,135,045 | G→A | 5.3% | R55C (CGT→TGT) | USA300HOU_RS05620 ← | Nicotinamide‑nucleotide adenylyltransferase |
RA | USA300TCH1516_ALE | 1,196,508 | C→A | 100% | S338Y (TCT→TAT) | ftsZ → | Cell division protein FtsZ |
RA | USA300TCH1516_ALE | 1,416,458 | A→T | 100% | I216N (ATT→AAT) | oppD_4 ← | Oligopeptide transport ATP‑binding protein OppD |
RA | USA300TCH1516_ALE | 1,513,930 | A→T | 100% | L413F (TTA→TTT) | USA300HOU_RS07375 → | hypothetical protein |
RA | USA300TCH1516_ALE | 1,532,200 | G→A | 6.9% | R223C (CGT→TGT) | USA300HOU_RS07470 ← | hypothetical protein |
RA | USA300TCH1516_ALE | 1,539,250 | C→G | 100% | intergenic (‑349/+37) | aroC ← / ← USA300HOU_RS07505 | Chorismate synthase/hypothetical protein |
RA | USA300TCH1516_ALE | 1,646,189 | G→C | 100% | A155G (GCA→GGA) | ypdF ← | Aminopeptidase YpdF |
RA | USA300TCH1516_ALE | 1,744,473 | C→T | 100% | A67T (GCT→ACT) | apt ← | Adenine phosphoribosyltransferase |
RA | USA300TCH1516_ALE | 1,789,619 | T→A | 9.4% | intergenic (‑100/+70) | gapA2 ← / ← coaE | Glyceraldehyde‑3‑phosphate dehydrogenase 2/Dephospho‑CoA kinase |
RA | USA300TCH1516_ALE | 2,010,363 | C→T | 100% | C146Y (TGT→TAT) | USA300HOU_RS10125 ← | Putative multidrug export ATP‑binding/permease protein |
RA | USA300TCH1516_ALE | 2,024,507 | C→A | 10.1% | A37E (GCA→GAA) | USA300HOU_RS10210 → | hypothetical protein |
RA | USA300TCH1516_ALE | 2,279,705 | G→A | 100% | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
RA | USA300TCH1516_ALE | 2,337,959 | C→T | 100% | intergenic (‑125/‑283) | adhR ← / → USA300HOU_RS11940 | HTH‑type transcriptional regulator AdhR/Hyaluronate lyase |
RA | USA300TCH1516_ALE | 2,343,438 | C→T | 49.6% | intergenic (+114/+250) | USA300HOU_RS11955 → / ← aldC_1 | 65 kDa membrane protein/Alpha‑acetolactate decarboxylase |
RA | USA300TCH1516_ALE | 2,455,692 | Δ1 bp | 100% | coding (1210/1260 nt) | lyrA → | Lysostaphin resistance protein A |
RA | USA300TCH1516_ALE | 2,455,698 | 2 bp→AT | 100% | coding (1216‑1217/1260 nt) | lyrA → | Lysostaphin resistance protein A |
RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | USA300TCH1516_ALE | 512620 = | 84 (1.300) | 52 (1.170) +40 bp |
35/180 | NT | 47.3% | intergenic (+688/‑6075) | recR/speA_2 | Recombination protein RecR/Arginine decarboxylase |
? | USA300TCH1516_ALE | 1998278 = | NA (NA) | intergenic (‑6/+3329) | USA300TCH1516_01882/USA300TCH1516_01883 | tRNA‑Val/tRNA‑Ala |