Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F13 I0 R1 125 11120.0 6942704 98.3% 6824678 135.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,056,962 C→A 24.5% F137L (TTC→TTA)  adk → adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,056,9620CA24.5% 108.7 / 5.8 94F137L (TTC→TTA) adkadenylate kinase
Reads supporting (aligned to +/- strand):  ref base C (16/47);  new base A (17/10);  total (37/57)
Fisher's exact test for biased strand distribution p-value = 1.57e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTACTACT  >  NZ_CP022686/4056824‑4057098
                                                                                                                                           |                                                                                                                                         
gcaggcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTAAGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGCCCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCAAGTTAAAATCa                                                                                                                                          >  3:455868/1‑140 (MQ=255)
 caggcagCCGCGATTAAAGAAGATGGCATCAATGTAGATAACGTTCTGGAATTCGACGTACCGTACGAACTGATTGTTGACCGTATCGAAGTCCGCCGCCTTCACGCGCCGTC‑TGGTCGTGTATATCACCTTAAAATCaa                                                                                                                                         >  7:703458/1‑140 (MQ=255)
 caggcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTATCACGATAAATTGaa                                                                                                                                         >  7:27737/1‑140 (MQ=255)
  agggagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAAt                                                                                                                                        <  7:432111/140‑1 (MQ=255)
  aggcagACGCGATCAAAGAAGCGGGCATCAATGTTGAGTACGTTCTGGAATGCGACGTACCGGACGAACTGATGGTTGCACGTCTCGTAGGCCGCCGCGGGCACGCGCCGTC‑TGGTCGGGTGTATCACGGTAAAGTCAAt                                                                                                                                        >  8:470926/1‑140 (MQ=255)
   ggcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAGCTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATAACGTTAAATTGaa                                                                                                                                         >  1:526874/1‑138 (MQ=255)
   ggcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGGTTAATTCaa                                                                                                                                         >  6:540247/1‑138 (MQ=255)
   ggcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTATCACGGTAAATTaaa                                                                                                                                         >  1:640949/1‑138 (MQ=255)
    gcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGCCCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTATCACGTTAAATTAAATcc                                                                                                                                      >  8:657988/1‑140 (MQ=255)
         cgcgATGAAAGAAGCGGGCATCATTGTTGATTACGGTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATccgcc                                                                                                                                   <  6:487103/138‑1 (MQ=255)
         cgcgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATccgcc                                                                                                                                   <  6:311811/138‑1 (MQ=255)
         cgcgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGa                                                                                                                                 <  4:684592/140‑1 (MQ=255)
              tGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTATAACGTTAAATTAAATACGCCGAAAGtt                                                                                                                            >  8:422908/1‑139 (MQ=255)
                    aagAGGGCATCAATGTTGATTACGTTCTGGAATTAGACGTACCGGAAGAACTGATGGTTGACCGTATCGTAGGACTCCGAGTTAACGCGCCCTC‑TGGGCGTGTTTATCAACGTAAATTAAATCCGCACTAAATAGAAGGc                                                                                                                      >  8:131339/1‑140 (MQ=255)
                     agCGGGCTTGAATGTTGATTACGTTCTGGATTTCGACGTACCGGACGAACTGATTGTTGACCGTAGCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAgg                                                                                                                       <  6:356062/138‑1 (MQ=255)
                      gCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGGC‑TGGTCGTGTTTATCACGGTAAAATCAATACCCCGAAAGTAGAAGGCaa                                                                                                                    >  4:3718/1‑140 (MQ=255)
                        gggCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGGTAAATTCAATCCGCCGAAAGGAGAAAGCaaaa                                                                                                                  >  3:245815/1‑139 (MQ=255)
                           cATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTAGTGTTTATAACGTTAAAATAAATACGCCGAAAGGTGAAGGGAAAtacg                                                                                                               >  4:55682/1‑140 (MQ=255)
                            aTCAATGTTGATTACGTTCTGGAATTCGACGTACCGGAAGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGATCACGCGCCGTC‑TGGTCGTGGTTATAAAGGTTAATTAAATACGCAGAAACTATCAAGCaaaaaa                                                                                                                >  5:544426/1‑135 (MQ=255)
                               aaTGTTGATTACTTTCTGTAATTCGACTTACCGGACGAAGTTATTGTTGACCGTATCGTCGTCCGCCGCTTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAATTAGACGGCAAAGACG‑ACGt                                                                                                          <  4:185424/140‑1 (MQ=255)
                                  tttttttACGTTCTGGAATTCCACGTACCGGACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCAGGCGCCGTC‑TGGTCGTGTTTATCAGTTTAAATTAATTCCGCCTAATGTTTAAGGCAAAGACG‑ACGTTAc                                                                                                       <  7:709697/135‑1 (MQ=255)
                                  tttGATTACGTTCTGTAATTCGACGTACCGGACGAACTTATTGTTGACCGTATTGTAGGCCGCCGCGTTCACGCGCCGCC‑TGGTCGTGTTTATCACTTTAAATTAAATCCGCCGAAATTAGAAGGCAAAGACG‑ACGtt                                                                                                         <  1:70228/137‑1 (MQ=255)
                                  tttGATTACGTTATGTAATTCTACGTACCGGACTAACTGTTTGTTTACCGTATCGTAGGCCGCCGCTTTCTCTCGCCGTC‑TGTTAATTTTTATCACGTTAAATTAAATCCGCCGAAATTATAAGGCAAAGACG‑ACGtt                                                                                                         <  6:839867/137‑1 (MQ=255)
                                       ttAGGTTCTGCAATTGGACGTACGGGACGAACTGATTGTTGACGGTATCGTAGGCCGCGGCTTTCACGCGCGGTC‑TGGTCTTGTTTATCACTTTAATTTCAATCCCCCCAAATTAGAAGCCAAAGACG‑ACGTTACCGGTg                                                                                                  <  8:702635/140‑1 (MQ=255)
                                        tACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGCCCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTTAAATAAAAACGCCGCAACTAGAACGCAAAAACG‑ACGTTTCCgcg                                                                                                   >  5:71112/1‑136 (MQ=255)
                                        tACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTTTAACGTTAAATTGAAACCGCCGAAAGTATAAGGCAAATAAG‑CCGTTTCCggg                                                                                                   >  1:158539/1‑137 (MQ=255)
                                        tACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTATCACGTTAAAATCAATCCGCCGAAAGTATAATGCAAAAACG‑ACGTTAACGGt                                                                                                   >  1:499620/1‑138 (MQ=255)
                                           gTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATAACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTgaaga                                                                                              >  8:823396/1‑140 (MQ=255)
                                           gTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCAAGCGCCGTC‑TGGTCGTGTTTATCACGTTTAATTAAATAAGACGAAAGTATAAGGCAAAGAAG‑ACGTTAACGGTgaaga                                                                                              >  3:656768/1‑140 (MQ=255)
                                            ttCTGGAATTCGACGTACCGTACGAACTGTGTGTTGACCTTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGGCGTGTTTATCACGTTACATTCAATCCGCCGAAAGTATAAGGCAATGACG‑ACGTTACCGGTgaag                                                                                               <  2:695410/138‑1 (MQ=255)
                                            ttCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATAACGTTAAATTCAATCCGCCCAAATTAGAACGCAAAGACC‑ACCTTACCggggcataa                                                                                             >  7:472628/1‑133 (MQ=255)
                                             tCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTATCACCTTAAAATAAATACGCAGAAATTAGAACGCAAAAAAC‑ACATTAACGGTGAAGAAc                                                                                            >  7:762242/1‑140 (MQ=255)
                                                 tatttCGAGGTAGCGGACTAACTGATTGTTGACCGTATCGTAGGCGGCGGCTTTCACGCGCCGTC‑TGGTGGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGAc                                                                                        <  3:413595/137‑1 (MQ=255)
                                                      cGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGGGTTTTTAACGTTAAATTCAATCCGCCGAAAGTTGAAGGCAAATACG‑ACGTTACCCGGGGAGAACTGACTAacc                                                                                   >  8:442616/1‑140 (MQ=255)
                                                        cggTACCGGACGACGTGATTGTTTCCCGTCTCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCCGGTTAAATTCAATCCGACGAAATTAGAAGGAAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGt                                                                                 <  8:482613/138‑1 (MQ=255)
                                                            aCCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATAACGGTAAATTAAATCCGCCGAAAGTAGAAAGCAAAGACG‑ACGTTTCCGGTGAAGAACTGACTACCCGTAAAg                                                                             >  4:712890/1‑140 (MQ=255)
                                                             cgggACGAACTTATTGTTGACGTTATCGTAGGCCGCCGCGTTCACGCGCCTTC‑TGGGCGTGTTTATGACTTTAAATTCAATCCGCCTAACGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGa                                                                            <  8:777785/138‑1 (MQ=255)
                                                             ccGGACTAACTGATTGTTGCCCGTATCGTCGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTTTTTATCAGGTTAAATTCAATCCGCCGAATGTCGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGa                                                                            <  4:301608/140‑1 (MQ=255)
                                                               ggACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTGCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAATTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGa                                                                            <  2:88382/138‑1 (MQ=255)
                                                               ggACGAACTGATTGTTGCCCGTATCGTAGGCCGCCGCGTTCACGCGCCGGC‑TGGTCGTGTGATTCCCGGTAAATTAACTGCGCCGCTAGGAGAAGGGAAAGAAG‑TCGTTTCCGGTGAATACCTGTCGAGACGTAAATAcc                                                                          >  4:381693/1‑139 (MQ=255)
                                                               ggACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTTCCCGTAAAGACg                                                                          <  4:376120/140‑1 (MQ=255)
                                                                 ccGAACTGTTTGTTGACCGTATCGTCGGCCGCCGCGTTCACGTGCCGTG‑TGGGCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTACAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                        <  8:608639/139‑1 (MQ=255)
                                                                 actaaatGATTGTTGACCGTAGCGTAGGCCGCCGAGTTCACGCGCCGTC‑TGGTCGTTTTTATCACGTTAAATTCAAACCGCCTAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                        <  7:255218/134‑1 (MQ=255)
                                                                 actaaCTGTTTGTTGACCTTATCGTCGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                        <  4:174466/137‑1 (MQ=255)
                                                                 actaaCTGATTGTTTACGGTTTCGTAGGCCGCCGGGTTCTCTCGCCGTC‑TGTGCGTGTTTATCACTTTAAATTCAATCCGCCGAAATTTGAAGGAAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                          <  2:845474/135‑1 (MQ=255)
                                                                 aCGAAGTGATTGTTGACGGTATCTTAGGCCGCCGCGTTCACGCGCGGTC‑TGGTGGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                        <  8:762242/140‑1 (MQ=255)
                                                                 aCGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAATTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                          <  2:681312/138‑1 (MQ=255)
                                                                 aCGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTGCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTATAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                        <  3:340812/140‑1 (MQ=255)
                                                                 aCGAACTGATTGTAGACAGTATCGTAGGCCGCCGCGTACACGCGCAGTG‑TGGTCTGGTTTATCACGTTAAATTCAAGCCGCCGAAAGTATAAGGAAAATACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                        <  4:523744/140‑1 (MQ=255)
                                                                       tGATTGTTTACTGTATCGTAGGCCTCCGCGTTCACGCGCCGTC‑TGTTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGaa                                                                  <  4:787879/140‑1 (MQ=255)
                                                                       tGATGGTTGACCGTATCGTAGCCCGCCGCGTTCAGGCGGCGTC‑TGGTGGTGTTTATCCCGTTAAATTCAATCCGCCTAAAGAAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGaa                                                                  <  8:473720/140‑1 (MQ=255)
                                                                         aTTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATACAATCCGCCTAAAGTATAAGGAAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGaa                                                                  <  5:92785/138‑1 (MQ=255)
                                                                           tgttgACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGGTTTTCACCTTTAATTAAAACCGCCCCAAATATCAGGGAAATACG‑ACGGTAACGGGGAAGAACTGACGTCCCCTTAAGCCGCTCACGAAgaga                                                              >  7:721148/1‑140 (MQ=255)
                                                                             ttgACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTTTCACGTTAAATTAAATCCGCCGAAATTAGAAGGCAAACACG‑ACGTTAACGGTGAAGAACTGACTACCCGTAAAGACGATAATGAAgaga                                                              >  1:438712/1‑138 (MQ=255)
                                                                              tgACCGTATCGTAGGCCGCCGCGTTCACGCGCGGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGAc                                                             <  5:210662/138‑1 (MQ=255)
                                                                               gACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCAAGTTAAATTAAAAACGCCGAAAGTAGAAGGCAAAGACG‑ACGTTAACGGTGAAGAAATGACTTCCCGTAAAGACGATCAGGAATAGACcgt                                                          >  3:704120/1‑140 (MQ=255)
                                                                                 ccGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTTTAACGTTAAATTGAATACGCCGAAAGTAGAACGCCAAGACG‑ACGGTACCGGTGGAGAACAGACTACCCGGAAAGACGCTCAGGAAGAGCCcgt                                                          >  6:547995/1‑138 (MQ=255)
                                                                                    tttCGTAGGCGGCCGCTTCCACGCGCGGTC‑TGTGCGTGTTTATCACTTTAAATTCAATCCGCCTAAATTAGAAGGAAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACcgtacg                                                       <  5:774112/136‑1 (MQ=255)
                                                                                     aTCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGGTAAATTCAATCCGCCGAAAGTATAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACAACCCGTAAAGACGATCAGGAAGAGAcccttcgg                                                      >  2:862746/1‑132 (MQ=255)
                                                                                          tggCCGCCGCGTTCACGGGCCGTC‑TGGTGGTGTTTATCAAGTTAATTTAAATCCGCCTAAAGTTGATGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                                 <  1:146085/137‑1 (MQ=255)
                                                                                          tggCCGCCGCGTTCACGCGCCGTA‑TGGTTGGTTTTATATCTTTATATTCAATCCGCCGAAAGTAGATGGCTAAGACG‑ACGTTACCTGTGAAGAACTGACTACCAGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                                 <  6:49645/137‑1 (MQ=255)
                                                                                          cggCCCCCGCGTTCACGCGCCGTG‑TGGGGGTGTTTATCACTTTAAATTAAATCCGCCGAAATTAGACGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                               <  7:429809/139‑1 (MQ=255)
                                                                                          aGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                                 <  2:156057/138‑1 (MQ=255)
                                                                                            gccgccGCGTTCACGCGCCGTC‑TGGTCGTGTTTTTCACGTTAAATTCAATACGCCGAAATTATAAGGCAAAGACG‑ACGTTTCCGGTGTAGAACAGACTACCCGTAAAGACGATCAGGAAGAGACCGTAAGTAAACGTCTg                                             >  8:391047/1‑140 (MQ=255)
                                                                                            gccgccGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑CCGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTg                                             >  4:718063/1‑140 (MQ=255)
                                                                                            gccgccGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCAAGTTAAATTAAATACGCCGAAAGTATAAGGGAAAGACG‑ACGTTACCGGGGAAGAACTGACTACCCGTAAAGACGCACAGGAAGAGACCCTACGTAAACGtatt                                             >  8:387122/1‑137 (MQ=255)
                                                                                             ccgccgCGTTCCGGCGCGGTA‑TGGGCTGGTTTATCATTTTAATTTCAATCCTCCTAAATTCTAAGGCAAACACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCt                                              <  5:176584/138‑1 (MQ=255)
                                                                                              cgccgcGTTCACGCGCCGTC‑TGGTCGTGTTTATGACGTTTAATTCAAACCGCCGAAATTAGAAGGCAAAGACGCCCGTTA‑CGGGGAAGAACTGACGAACCGTAATGACGCTCAGGAATAGCCCCTACGTAAACCTGTGGt                                           >  8:24024/1‑140 (MQ=255)
                                                                                              cgccgcGTTCACGCGCCGTC‑GGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGt                                           <  8:210905/140‑1 (MQ=255)
                                                                                                  gcgTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑CAGTTAACGGTGCAGCTCTTCATACCCCTACAGACGATCATGAAGAGACCCTACGTAAACGTCTGGTTg                                         >  6:501149/1‑138 (MQ=255)
                                                                                                  gcgTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTACATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGGGAAGCACTGACAACCCGGAAAGACGCTCACGAATCGACCCTACCTAAAAGTCTGGTTg                                         >  6:130710/1‑138 (MQ=255)
                                                                                                  gcgTTCACGCGCCGTC‑TGGTCGTGTTTATAACCGTAAATTAAAACCCCCGAAAATAGAACGCAAAGACG‑CCCTTTCCGGTGAATCAAAGACAACCCGGAAAAACGATAATGAAGACACCGGAAGTAAACGTCGGGttt                                         >  6:89620/1‑137 (MQ=255)
                                                                                                   ggTTCACGCGCCGTC‑TGGTCGGGTTTATCACGTTAATTTCAATCCGCCCAATGTAGAAGGAAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                      <  8:721148/139‑1 (MQ=255)
                                                                                                   cgTTCACGCGCCGTA‑TGTTCGTTTTTATCACGTTAAATTCAATCCGACGAAAGTATAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGa                                        <  1:175045/138‑1 (MQ=255)
                                                                                                     ttCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                      <  1:456018/138‑1 (MQ=255)
                                                                                                        ccgcgCCGTC‑TGGTCGTGTTTATCACGTTAATTTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCAt                                 <  3:703522/139‑1 (MQ=255)
                                                                                                          gcgcGGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGACAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCa                               <  4:542872/140‑1 (MQ=255)
                                                                                                          gcgcCGTG‑TGGTCTGTTTTATCTCTTTAAATTCATTCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCag                                 <  6:437535/138‑2 (MQ=255)
                                                                                                           ggcCGTCGTGGTTGTTTTTATCACGTAATATACAATCCGGCTAAAGTTTATGGCTAAGACG‑ACGTTACCGGTGAATAACTGACTACCCGTAATGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGTATACCAt                                 <  2:545600/137‑1 (MQ=255)
                                                                                                             cggTC‑GGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑CCGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                            <  8:251271/138‑1 (MQ=255)
                                                                                                             ccGTC‑TGGTCGGGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGCAAACACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                            <  8:67306/140‑1 (MQ=255)
                                                                                                             ccGTA‑TGGTCGTGTTTATCACGTTAAATTAAAGCCGCCGAAAGTAGAAGTCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                            <  7:588602/140‑1 (MQ=255)
                                                                                                               ggc‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAACGTAGACGGCAAAGACG‑CCGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGa                          <  8:153274/138‑1 (MQ=255)
                                                                                                                tC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTATATCGAAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGAc                         <  7:141300/140‑1 (MQ=255)
                                                                                                                      gcGTGTTTATAACGTTAAATTCAAACCGCCGAAAGTAGACGCCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCAc                    <  8:731042/139‑1 (MQ=255)
                                                                                                                           tttATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAGGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTg               >  7:161751/1‑140 (MQ=255)
                                                                                                                           gttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGGGTAAAACCATCAGATGCCTGCACCGcag               >  4:54169/2‑138 (MQ=255)
                                                                                                                            tattCACGTTTAATTAAATCCGCCTAAATTCGAAGGCAAAGACG‑ACGTTACCGGTGAAGATCTGACTACCCTTAAAGACGATCAGTAAGAGACCGTAAGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGa              <  8:664318/137‑1 (MQ=255)
                                                                                                                              aTCAGGTTAAATTCAATCCGCCGAATGTAGAAGGCAAAGACG‑ACGGTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGa              <  5:562529/138‑1 (MQ=255)
                                                                                                                              aTCACGTTAATTTCAATCCGCCGAACTTCGATGCCACCGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGAACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGa              <  5:807052/138‑1 (MQ=255)
                                                                                                                                   gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGcta       <  8:347691/140‑1 (MQ=255)
                                                                                                                                   gTTAAATTCAATCCGCCCAAAGTACAAGGCAATGACG‑ACGTTACGGGTGAAGAACTGACTACCCGTAAAGAAGATCAGGTAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGc         <  1:571315/138‑1 (MQ=255)
                                                                                                                                    taaaaTTCAATCCGCGGAATGTAGCAGGCATAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGct        <  6:165315/136‑1 (MQ=255)
                                                                                                                                        aTTCAATCCCCCTAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTactact  <  4:562989/140‑1 (MQ=255)
                                                                                                                                        aTTAAATCCCCCGAAAGTACAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTactact  <  8:571264/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GCAGGCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTC‑TGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACG‑ACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTACTACT  >  NZ_CP022686/4056824‑4057098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A