Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F2 I0 R1 184 7301.6 8720326 98.4% 8580800 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,056,962 C→A 19.1% F137L (TTC→TTA)  adk → adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,056,9620CA19.1% 249.6 / 13.9 159F137L (TTC→TTA) adkadenylate kinase
Reads supporting (aligned to +/- strand):  ref base C (25/96);  new base A (22/13);  total (50/109)
Fisher's exact test for biased strand distribution p-value = 5.00e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

AGGCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTAC  >  NZ_CP022686/4056826‑4057094
                                                                                                                                        |                                                                                                                                    
 ggcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTAACGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACCCGCCGTCTGGTCGTGTTTATAACGTTTAATTaaa                                                                                                                                    >  6:558547/1‑138 (MQ=255)
  gcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACTTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTAACGCGCCGACAGGTCCTGGTGATAACCttaaattaaaaaa                                                                                                                                 >  7:228186/1‑137 (MQ=255)
     gACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTATGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATccgcc                                                                                                                              <  2:1271/140‑1 (MQ=255)
       cgcgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGa                                                                                                                            <  4:817313/140‑1 (MQ=255)
            tGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAg                                                                                                                         <  6:949815/138‑1 (MQ=255)
                     cGGGCATCAATGTTTATTACGTTCTGGAATTCGACGTACCGGACGAACTTATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCa                                                                                                                <  6:120956/138‑1 (MQ=255)
                       ggCATCAAAGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGATGACCGTATCGTAGGCCGACGCGTTCACGCGCCGTCTGGTCGTGTATTTAACGGTAAATAAAAAACGCCCAAATTAGAAGTCAAAga                                                                                                            >  7:703628/1‑140 (MQ=255)
                         cATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAgacg                                                                                                          >  3:73358/1‑140 (MQ=255)
                               tGTTGATTGCGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTa                                                                                                    >  4:1034727/1‑140 (MQ=255)
                                tttGATTAGGTTCTGTAATTCGACGTACGGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCAGGCGCCGTGTGGTCGTGTTTATCTCGTTAAATTCAATCCGCCGAAAGTAGACGCCAAACACGACGtt                                                                                                     <  5:304726/137‑1 (MQ=255)
                                tttGAATACGTTATGTAATTTGACTTACCGGACTAACTGATTGTTGACCGTAGCTTAGCCCGCCGCGTTCACGCGCCGTCTGGTGGTGTTTATCACGTTAAATTAAATCCGCCTAAAGTACATGCCAAACACGACGTTAc                                                                                                   <  8:14065/139‑1 (MQ=255)
                                gTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCGGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTAc                                                                                                   <  4:22324/140‑1 (MQ=255)
                                  ttaTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCg                                                                                                 <  8:715486/138‑1 (MQ=255)
                                  tGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCg                                                                                                 >  7:240252/1‑140 (MQ=255)
                                    aTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATACCCCCAAATTAGAAGGCAAAGACGACGTTTCCggg                                                                                               >  1:812164/1‑139 (MQ=255)
                                    aTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGGTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATACGCCGAAAGTAGAAGGCAAAGACGCCGTTACCGGt                                                                                               >  4:625131/1‑140 (MQ=255)
                                    aTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGGTCACGCGCCGTCTGGTCGTGTGTATCACGGTTAATTAAATACGCCGAAAGTTGAAGGCAAAGGCGCCTTTACCggg                                                                                               >  4:120594/1‑139 (MQ=255)
                                     ttACGTTCTGTAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCgg                                                                                                <  5:945614/138‑1 (MQ=255)
                                      tACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTAACACGTTAAATTAAATACGCCGAAAGAAGAAGGCAAAGAACACGTTACCGGGga                                                                                             >  2:641905/1‑140 (MQ=255)
                                      tACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTAACGCGCCGTCTGGTCGTGGTTATACAGATAAATTAAAAACACCGAAAGAAGAAAGAAAAACAGCCGTTACCAGt                                                                                               >  5:886307/1‑138 (MQ=255)
                                       aCGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTg                                                                                              <  6:528020/138‑1 (MQ=255)
                                        cGTTCTGTAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCGGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTgaag                                                                                           <  2:383651/140‑1 (MQ=255)
                                         gTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTTAATTCAATCCGCCGAAATTAGAAGGCAAAGACGCCGTTACCGGGgaaga                                                                                          >  2:712888/1‑140 (MQ=255)
                                         gTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTgaaga                                                                                          >  7:840730/1‑140 (MQ=255)
                                          ttCTGTAATTCGACGTACCGTACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGACGGCAAAGACGACGTTACCGGTgaagaa                                                                                         <  2:89153/140‑1 (MQ=255)
                                          ttCTGGAATTCGACGTACCGTACGAACTGTTTGTTGACCGTAGCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTgaagaa                                                                                         <  8:331637/140‑1 (MQ=255)
                                           tCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCAAGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAATACGACGTTACCGGTGAAGAAc                                                                                        >  8:254146/1‑140 (MQ=255)
                                           tCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGGTCACGCGCCGGCTGGTCGTGGTTATCAAGTTTAATTAAATAAGCCGAAAATAGGAGGCCAATCCCACGTTTCAGGTgtaaa                                                                                          >  5:675535/1‑134 (MQ=255)
                                             tGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGCAAGTAGAAGGCAAAGACGACGGTACCGGTGAAGAAc                                                                                        >  6:294386/1‑138 (MQ=255)
                                              ggAATTCGACGAACCGGACGAACTTATTGTTGACCGTATCGTCGGCCGCCGCGTTCACGCGCCGTCTGGGCGTGTTTATCCCGTTAAATTCAAACCGCCGAAAGTAGAAGCCAAAGACGACGTTACCGGTGAAGAACTGa                                                                                     <  1:907813/140‑1 (MQ=255)
                                                aaTTCGACTTACCGGACGAACTGATTGGTGACCTTATCGTCGGCCGCGGGGTTCCCGCGCCGTATGGTCGTGTTTATGACGTTAAATTCAATCCGCCTAAAGTATAAGCCAAAGACGACGTTACCGGTGAAGAACTGACt                                                                                   <  7:853876/140‑1 (MQ=255)
                                                aaTTCGACGTACCGGACGAACTGATTGTGGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTAAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACt                                                                                   <  1:712888/140‑1 (MQ=255)
                                                aaTACGACGTCCGGGACGAACTGATTGTGGACCGAATCGTCGGCCGCCGCGTTCACGCGCCGTCTGTTCGGGTTTATCACGTTAAATTCAAGCCGCCTAACGTAGAAGGCAAAGACTACGTTACCGGTGAAGAACTGACt                                                                                   <  3:832007/140‑1 (MQ=255)
                                                       cGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTGAATCCGCCGCAATTAGAAGGCAAAGACGCCGTTACCGGTGAAGCACGGACTACCCGTa                                                                            >  2:485575/1‑140 (MQ=255)
                                                        gaaCCGGACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGGGCCGTCTGGTCGTGTTTATCCCGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTaa                                                                           <  8:935397/138‑1 (MQ=255)
                                                         tACCGGTCGAACTTATTGTTTCCCGTATAGTGGTCCGCCGGGTTCACGGGGCTTCTGGTCGTTTTTATCCCTTTAAATTAAATCCGCCTAAATTAGACGGAATAGACGACATTACCGGTGAAGAACTGACTACCCGTaaa                                                                          <  8:219525/140‑1 (MQ=255)
                                                         tACCGGACGAACTGATTGTGGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTaaa                                                                          <  8:555267/140‑1 (MQ=255)
                                                          aCCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTTTTACGGTTAATTAAATCCGCCCGAAGTTGAAGGGAAAGACGACGTTTACGGGGCAGGACGGACTAACCGTaaaa                                                                         >  3:645723/1‑139 (MQ=255)
                                                           ccGGAGGAACTGATTGTCGACCGTTTCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGa                                                                        <  4:721470/140‑1 (MQ=255)
                                                           ccGGACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGa                                                                        <  7:437997/140‑1 (MQ=255)
                                                           ccGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGa                                                                        <  1:511460/140‑1 (MQ=255)
                                                             ggACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCTGTCACGCGCCGTCTGGGCGTGTTTATCACGTTAAATTCAATCCGCCCAATGTATAAGGCAATCACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                      <  4:61466/140‑1 (MQ=255)
                                                               actaaCTGATTGTTGACCGTATCGTAGGCCGCCGCTTGCACGGGCGGTCTGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTAGAAGCCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                      <  5:382722/135‑1 (MQ=255)
                                                               accaaCTGATTGTTGACCGTAGCGGTGGCCGCCGCGTTCACGCGCCGTGTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGATGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                    <  2:785300/137‑1 (MQ=255)
                                                               aCGAACTGTTTGTTGACGGTATCGTAGGCCGCGGCGTTCACGCTCCGTCTGGTCGTTTTTAACACTTTAAATTCAAACCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                    <  1:733569/140‑1 (MQ=255)
                                                               aCGAACTGATTGTTGACCTTATCGTAGGCCGCGGGGTTCACGCGCCGTGTGGTGGTTTTTATCACTTTAAATAAAACCCGCCGAAAGTAGAAGGCAAACACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                    <  3:307948/140‑1 (MQ=255)
                                                               aCGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACTTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                    <  3:459541/140‑1 (MQ=255)
                                                                   aCTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAgg                                                                >  2:617888/1‑140 (MQ=255)
                                                                     tGATTGTTGACCGTAGCGTAGGCCGCCGCGTGCACGCGCCGTGTGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAATTAGAAGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAgg                                                                <  5:54111/138‑1 (MQ=255)
                                                                         tgttgACCGTATCGTAGGCCGCGGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTCGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAgaga                                                          <  2:460472/140‑1 (MQ=255)
                                                                           ttgCCCGTCTCGTTGGCCGCCGCTGTCACGGGCCGTCTGGTCGTGTTTCTAACGTTAAATTCAATCCGCCGAAAGTAGATGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAgaga                                                          <  5:616863/138‑1 (MQ=255)
                                                                            tgACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTCACGTTTAATTAAATCCGCCGAAAGGAGAAGGCAAAGACGACGGTACCGGTGAAGAACTGACTACCCCGAAATACGAACACGGAGAGAccc                                                       >  1:1057639/1‑139 (MQ=255)
                                                                            ggACCTTCTCGTTGGCCGCCGCGTTCACGGGGCGTGGGGGCTTGTTTCACACGTTAAATTCAACCCGCCGAACGTATAAGGCAATCACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACcg                                                       <  1:525352/139‑1 (MQ=255)
                                                                             gACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATCACGTTAAATTCAATACGCCGAAAGTAGAAGGCAAATACGACGTTACCGGGGAAGAACTGACTACCCGTAAAGACGATAAGGAAGAGACcgt                                                      >  8:696022/1‑140 (MQ=255)
                                                                                 gTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAAATAAATCAGCCCCAAGTAGAAGGCAAACCCGCCGTTACCCGTGAAGACCTGACTACCCCTAAAGACGATCAGGAAGAGCCcgtacgg                                                  >  2:790598/1‑139 (MQ=255)
                                                                                 gTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACCTTAAATAAAAACCGCCCAAAGTAGAAGGCAAAGACCTCGTTACCCGTGAAGAACTGACAACCCGTAAAGTCGATCAAGAAAAGAAcctacgt                                                  >  8:209335/1‑140 (MQ=255)
                                                                                   aTCGTAGGGCGGCGGGTCCACGCGCGGTCTGGTGGTGTTAATCACGTTAATTTCACTCCGCAGAAAGTAGAAGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAACGACGATCAGGAAGAGACcgtacgt                                                  <  6:650504/138‑1 (MQ=255)
                                                                                        cggCCGGCGCGGTCACGGGCCGTCTGTGCGCGGTTATCCCGATAAATCCAATCCGCCGAACGACTCCGCCAACGACGACGTTACCGGTGACGAACGGACTACCCGTAAAGACGATCAGGAAGAGACCGTCCGTACACggc                                           <  8:845611/139‑3 (MQ=255)
                                                                                        cggCCGCCGCGTTCCCGCGCCGTCTGGTCGTGTTTATAACGTTAAATTCAATCCGCCTAAAGTACAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                           <  3:16531/139‑1 (MQ=255)
                                                                                        cggCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                           <  4:347585/139‑1 (MQ=255)
                                                                                        cggCCGCCGCGTACACGCGCCGTCTGGTCGTGTTTATCCCGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAACACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                           <  1:956679/139‑1 (MQ=255)
                                                                                        cggCCCCCCCGTTCACGCGCCGTAGGTGCTTTTTTATCACGTAAAATACAATCCCCCTAAATTACAAGACAAAGACGACCTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                             <  5:220714/137‑1 (MQ=255)
                                                                                        aGGCCGCCGCGTTAACGCGCCGTCTGGTCGTGTTTATAACTTTAAATTCAATCCTCCTAAAGTATAAGTCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTCAAGACGATAAGGAAGAGACCGTACGTAAACGTc                                           <  4:987404/140‑1 (MQ=255)
                                                                                               cgcgTTCACGGGCCGTCGGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTCGACGCCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGa                                    <  4:61480/140‑1 (MQ=255)
                                                                                                gcgTTCACGCGCCGTCTGGTCGTTTTTATCACTTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGaa                                   <  1:1125789/140‑1 (MQ=255)
                                                                                                gcgTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGaa                                   <  3:1044613/140‑1 (MQ=255)
                                                                                                 ggTTCACGCGCCGGCGGGTCTTGTTTATCCCGTTAAATTCAATCCGCCGAAAGTAGATGGAAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                  <  7:539361/139‑1 (MQ=255)
                                                                                                 cgTTCCCGCGCCGTCTGGTCGTGTTTATCAGGTTAAATTAAATCCGCCGAAAGTAGAAGCCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                  <  1:415725/140‑1 (MQ=255)
                                                                                                 cgTTCACGCGCCGTGGGGTCTTGTTTATCTCTTTAATTTCATTCCGCCGAAATTTGATGGCAAAGACGACGTTAACGGTGACGAACTGACTACCCGTAAAGACGATCAGGTAGAGACCGTTCGTAAACGTCTGGTTGAAt                                  <  4:1082834/140‑1 (MQ=255)
                                                                                                   ttCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATAc                                <  3:383631/140‑1 (MQ=255)
                                                                                                     cACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGCCGTTACCGGTGAAGAACTGCCTACCCGGAAAGACGATCAGGAAGAGACCGTTCTTAAACGTCTGGTTGAATACCa                              >  4:981626/1‑140 (MQ=255)
                                                                                                     cACGCGCCGTCTGGTCGTGGTTATCACGTTTAATTAAATCCGCCGCAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCCTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCa                              >  4:897147/1‑140 (MQ=255)
                                                                                                      ccgcgCCGTCTGGGCGTGTTTAGCACGTTAAATTAAAGCCGCCCAACGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATAcc                               <  5:484560/137‑1 (MQ=255)
                                                                                                      atgcgcCGGCTGGTCGTGCTTATCACGTTAAATTCAATCCCCCGAACGACGACGCCAAACCAGACGTTACCGGTGAAGAACTGACTACCCGTATAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATAcc                               <  5:186189/136‑1 (MQ=255)
                                                                                                        gcgcCGGCTGGGCGTGTTTATAACGTTAAATTCAATCCGCCGAAAGTCGAAGCCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCAt                             <  5:156613/138‑1 (MQ=255)
                                                                                                           ccGTCTGGTTGTTTTTTTACTGTTAAAGTCAATCCGCCTAATGTAGTAGGCAAAGACGACGTTACGGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGGTGAATACCATCAGAt                        <  8:624607/140‑1 (MQ=255)
                                                                                                           ccGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                        <  4:1018317/140‑1 (MQ=255)
                                                                                                           ccGTCTGGTCGTGTTTAACACGTAAAATTCAATCCGCCTAAAGTCCAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                        <  1:236417/140‑1 (MQ=255)
                                                                                                              tCTGGTCGTGTTTATCACGTTAAATTCACTACGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTTCCCGTAAAGACGATAAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATgcc                     >  4:476937/1‑138 (MQ=255)
                                                                                                                tGGTCGTGTTTATCACCTTAAATTCAATCCGCCGAAACTAGAAGGCAAAGCCGACCGTACCGGGGTAGAACTGACTACCCCGAAAACCCCACACGAAGAGACCGTACGAAAACGTCTGGGTGAATACCATCAGAAGAc                     >  5:419084/1‑138 (MQ=255)
                                                                                                                   gggtgtTATTCACTTTAAATTCAATCCCCCGATCGTACAAGCAAATGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGc                  <  5:805284/136‑1 (MQ=255)
                                                                                                                     gtgtTTATCACGTTAATTTCAATCCGCCGAACGTACAAGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTGGAATACCATCAGATGACTGCACCg              <  2:508441/140‑1 (MQ=255)
                                                                                                                     gtgtTTATCACGTTAAATTCAAACCTCCCAAAGTAGAAGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCAc                <  6:527750/138‑1 (MQ=255)
                                                                                                                      tggttttCACGTTAAATTCCATCCGCCGAAAGTAGAAGGCAAAGACGGCGGTACCGGTGCAGAACTGACGACCCGGAAAGCCCAACAGGTAGAGCCCGTACGTAAACGTCTGGTTGTATAACATCAGATGACTGCACCGc             >  8:166400/7‑140 (MQ=255)
                                                                                                                      tggttATCACGTTAAATTCAATCCGCCGAAAGGAGAAGGCAAAGCCGCCGTTACCGGTGAAGAACTGCAGACCCGGAAACACGATCAGGGAGCGACCGTACGTAAACGTCTGGTTGAATACCCTAAGATGACATCACCGc             >  4:820846/4‑140 (MQ=255)
                                                                                                                       ttttATCAATTTAAATTAATTCTGCCGAACTTAGAAGGCAAAGAAGACGTTACCGGTGCAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCt            <  7:671733/139‑1 (MQ=255)
                                                                                                                         ttATCACGTTAAATTCATTCCGCCGAAAGTCGAAGGCAACGCCGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGa          <  2:586490/140‑1 (MQ=255)
                                                                                                                         ttATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGAAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGa          <  4:484707/140‑1 (MQ=255)
                                                                                                                          tATCACGTTAAATTCAAACCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTg           >  6:786154/1‑138 (MQ=255)
                                                                                                                           aTCACGTTAAATTCAATCCGCCGAAAGTAGACGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATc        <  4:73355/140‑1 (MQ=255)
                                                                                                                              aCGTTAAATTAAATCCGCCTACAGTAGACTGCAAAGACGACGTTACCGGGTCAGAACTGACTACCCGTAACGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGc     <  2:831077/140‑1 (MQ=255)
                                                                                                                                gtaaaaTTCATCCCGCCGAACGTACAAGGCAAACACGACGTTCCCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGcta   <  7:691906/137‑1 (MQ=255)
                                                                                                                                gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACt                                                                                       >  7:1032574/1‑56 (MQ=255)
                                                                                                                                gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACt                                                                                       <  8:1032574/56‑1 (MQ=255)
                                                                                                                                gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGcta   >  1:1009905/1‑140 (MQ=255)
                                                                                                                                gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGcta   >  1:62147/1‑140 (MQ=255)
                                                                                                                                gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGc     <  6:154970/138‑1 (MQ=255)
                                                                                                                                gTTAAATACAATCCGCCGAACGTAGACGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGcta   <  7:55241/140‑1 (MQ=255)
                                                                                                                                 ttAAATTCAATCCGCCGAAAGTAGAAGCCAATGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGctac  <  2:1075345/140‑1 (MQ=255)
                                                                                                                                 ttAAAATCAATCCGCCCACAGTAGACTGCACAGACTCCGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGctac  <  8:634408/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
AGGCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTAC  >  NZ_CP022686/4056826‑4057094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A