Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F2 I0 R1 166 6951.0 8192394 98.4% 8061315 136.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,056,962 C→A 21.8% F137L (TTC→TTA)  adk → adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,056,9620CA21.8% 157.1 / 6.7 130F137L (TTC→TTA) adkadenylate kinase
Reads supporting (aligned to +/- strand):  ref base C (24/68);  new base A (18/13);  total (49/81)
Fisher's exact test for biased strand distribution p-value = 1.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTACT  >  NZ_CP022686/4056828‑4057095
                                                                                                                                      |                                                                                                                                      
gcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATCACGGTTAATTCAAtac                                                                                                                                   >  8:609575/1‑138 (MQ=255)
gcagACGCGATGAAAGAAGCGGGCATCAATGATGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACAGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTTGTCGTGTTATTCACGTGAAAATCAATcc                                                                                                                                   >  2:978870/1‑140 (MQ=255)
   gACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATGCCGGTTAATTAAATccgcc                                                                                                                                >  1:818312/1‑140 (MQ=255)
   gACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATACCGGTAAATTAAATacgcc                                                                                                                                >  2:514768/1‑140 (MQ=255)
    aCGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATAACGTTAAATTAAAAacgccc                                                                                                                               >  7:608177/1‑139 (MQ=255)
     cgcgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTGTTTAACCTTAAATTAAAAACGCCGa                                                                                                                              >  5:641607/1‑140 (MQ=255)
          tGAAAGAAGCGGGCATAAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTTACCGTATCTTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTa                                                                                                                         <  5:990886/140‑1 (MQ=255)
           gAAAGAAGCGGGCATCACTGTTGATTACGTTCTGGAATTCGACGTACAGGACGAACTGATTGTATACCTTATCGTAGGCCGCCGCGTTCAAGCGCAGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTat                                                                                                                        <  7:768780/140‑2 (MQ=255)
           gAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAg                                                                                                                        <  7:930968/140‑1 (MQ=255)
           gAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGGAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAg                                                                                                                        <  1:1024388/140‑1 (MQ=255)
               taagGGGGAATCAATGTTTATTACGTTCTGTAATTCGACGTACCGGACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCAGTTTAAATTAAATCGGCCGATAGTAGATgg                                                                                                                    <  6:452203/139‑1 (MQ=255)
                aagCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTCACGTTAAATTCAATCCGCCGCAAGTAGAAGGc                                                                                                                   >  7:912992/1‑140 (MQ=255)
                aagCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGACGGc                                                                                                                   <  8:335985/140‑1 (MQ=255)
                  gCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCaa                                                                                                                 >  1:760016/1‑140 (MQ=255)
                   ggggCATCAATGTTTATTACGTTGTGGATTTCGACGTACGGGACTAACTGTTTGTTGACCGTAGGGTAGGCCGCCGGGTGCAGGCGCCGTCTGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTAGATGGCaaa                                                                                                                <  4:159055/139‑1 (MQ=255)
                           aaTGTTGATTACGTTCTGGAATTCGACGTACCGGACTAAATGATTGTTGACCTTATGGTAGGCCGCCGGGTTCACGCGCGGTCTGGTCGTGTTTATCACGTTATATTAAATCCGCCTAAAGTAGAAGGAAAAGACGACGt                                                                                                        <  1:659944/140‑1 (MQ=255)
                              tttGATTACGTTCTGTAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTCGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑Ac                                                                                                    <  3:234998/139‑1 (MQ=255)
                              gTTTATTAGTTTCTGGATTTCGACGTACCGGACTAACTTATTGTTGACGTTATCGTAGGCCGCCGCGTACACGCGCCGTCTGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTATACGGCAAAGACGACGTT‑Ac                                                                                                    <  8:379428/140‑1 (MQ=255)
                              gTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTAGCGTAGGCCGCGGCGTTCAGGCTCCGTCTGGTCGTTTTTATAACGTTAAATTCAATCCGCCGAAAGTATACGGAAAAGACGACGTT‑Ac                                                                                                    <  6:777387/140‑1 (MQ=255)
                              gTTGATTACGTGCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑Ac                                                                                                    <  2:573452/140‑1 (MQ=255)
                               ttGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCCCGCGCCGTCTGGTCGTGTTTATAACGTTAAATTCAATCCGCCGAAATTATAAGGCAAAGACGACGGT‑aac                                                                                                   >  6:137643/1‑138 (MQ=255)
                               ttGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGAGCCGTCTGGTCGTGTTTATCACGTTTAATTAAATACGCCAAAAGTATAAAGCAAAGACGACGTT‑Tcc                                                                                                   >  3:773953/1‑140 (MQ=255)
                                  aTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGTTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTATAAGGAAAATACCACGTT‑TCCGGt                                                                                                >  4:779910/1‑140 (MQ=255)
                                  aTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAAATAAATCCGCCCAAAGTTGCAGGCAAATCCGCCGGT‑Acccgg                                                                                                >  4:810848/1‑137 (MQ=255)
                                  aTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGGCTGGTCGTGTTTATCACGTTTAAGTCAATCCGCCGCAAGGATAAGGCAAAGCCGACGGT‑GCCggg                                                                                                >  8:872481/1‑139 (MQ=255)
                                   ttACGTTCTTTAATTCGACGTAACGGACTAACTGATTGTGGACCGTATCGTAGGCCGCAGCGTTCACGCGCCGGCTGGTCGTGTTTATCACTTTAATTTCAATCCGCCTAAATTAGAAGGAAAAGACGACGTT‑ACCGGTg                                                                                               <  1:444200/140‑1 (MQ=255)
                                   ttACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCTTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAAGCCGCCTAAATTATAAGGAAAAGACGACGTT‑ACCGGTg                                                                                               <  8:378288/140‑1 (MQ=255)
                                   ttACGTTCTGGAATCCGACGTACCGTACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAATGTAGAAGGCAAAGACGACGTT‑ACCGGTg                                                                                               <  3:185266/140‑1 (MQ=255)
                                      cGTTCTGGAATTCGCCGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAACGACGACGTT‑ACCGGTgaag                                                                                            <  2:758852/140‑1 (MQ=255)
                                      cGTGCTGGAATTCGACGTCCCGGCCGAACTGATTGTTGACCGTAGCGTAGGCCGCCGCGGTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGCAAGTAGACGGCAATGACGACGTT‑ACCGGTgaag                                                                                            <  6:766707/140‑1 (MQ=255)
                                       gTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATACCGTTAAATTAAATCCGCCGAAAGTAGAAGGCCAATAAGACCGT‑ACCCGTgaaga                                                                                           >  6:38991/1‑140 (MQ=255)
                                        ttCTGGAATTCGACGTACCGGACGAACTGATTGTTGCCCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTgaagaa                                                                                          <  6:477140/140‑1 (MQ=255)
                                         tCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTGTCACGTTAAAATATATACGCCGAAAGTAGCACGCTCAGAAGACGTT‑GCCGGAGAGGAAc                                                                                         >  3:898156/1‑140 (MQ=255)
                                         tCTGGAATTCGACGTACAGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCCTTCACGCGCCGTCTGGTCGTGGTTTAAACCTTAAAATTAAAAAGCCGAAAGTAGAAAGCAAAAACGAAGTTAACCGGTaaaaaa                                                                                          >  3:709887/1‑140 (MQ=255)
                                            ggAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATAACGTTAAATTCAATCCGCCGCAAGTAGAAGGGCAAGACGACGGT‑ACCGGGGAAGAACTgg                                                                                      >  2:675942/1‑139 (MQ=255)
                                             gAATTCGACGTCCCGCACGAACTTATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGAc                                                                                     <  4:147275/140‑1 (MQ=255)
                                             gAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATGACGTTAAATTGAATCCCCCGAAAGTATAAAGCAAAGACCACGTT‑ACCGGGGAATAACAGAc                                                                                     >  4:540306/1‑140 (MQ=255)
                                              aaTTCGACGTCCCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGGCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACt                                                                                    <  3:429585/140‑1 (MQ=255)
                                              aaTTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGACGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACt                                                                                    <  2:486643/140‑1 (MQ=255)
                                                ttCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATTACGGTAAAATTAATCACCCCAAAGTATAAGGCAAAGCCCACGGT‑ACCGGTGAAGAACAGACTaa                                                                                  >  1:416973/1‑139 (MQ=255)
                                                  cGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTGATAACGTTAAAATAAATCCGCCGAAATTAGAAGGGAAAGACGACGTT‑ACCGGTGTAGAACTGACAAccc                                                                                >  8:170787/1‑140 (MQ=255)
                                                   gACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATGACGTTTAATTCAATACGCCGAAATGAGAAGGCAAATACGCCGGT‑ACCGGTGAAGAACTGACTAcccc                                                                               >  3:758544/1‑139 (MQ=255)
                                                     gttACCGGACTAACTGTTTGTTGACCGTATGGTCGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACTTTAAATTCAATCCGCCTAAATTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTa                                                                             <  5:692430/138‑1 (MQ=255)
                                                     cGTACCGGACGAACTGATTGTTGCCCGTATCGTAGGCCGCCGCGTTCCCGCGCCTTCTGGTCGTGTTTATCACGTTAAATTCAACCCGCCTAAAGTACACGGAAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTa                                                                             <  7:51760/140‑1 (MQ=255)
                                                        aCCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATCAACTTTAATTCCAAACGCCGAAAGTTGAAGGCAAAAACGACGTT‑AACGGTGAAGGACTGACTACCCCTaaaa                                                                          >  8:171604/1‑139 (MQ=255)
                                                         ccGTACGAAGTGATTGTTGCCAGTATGGTAGGCAGCAGGTGTCCGTCGCCGTCTGGTCGTGTTTATCACGTTAAATAAAATCCCAATAAAGTATAAGTCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGa                                                                         <  4:140492/140‑1 (MQ=255)
                                                         ccGGACGAACTGATTGTTTACCGTCTCGTAGGCCGCCGCGTTCACGCGCGTTCTGGTCGTGTTTATCACTTAAAATTCATTCCGCCGATTGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGa                                                                         <  2:27215/140‑1 (MQ=255)
                                                           gtactaaCTGATTGTTGTCGGTATGTTAGGCCGCCGCGTTCTCGGGCCGTCGGGTGGGGTTTTTCATGTTAAATTCATTCGGCGTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                       <  8:353625/135‑1 (MQ=255)
                                                           ggACTAACTGATTGTTGACCGTATCGTCGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTATAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                       <  3:424944/140‑1 (MQ=255)
                                                             cccatcTGATTGTTGACCGTATCGTCGCCGGCCGCGTTCACGGGCCGTCTGGTCGTGTTTCTCTCTTTAAATTATATCGGCGGAATTTATAAGGAAAAGACGACGTT‑ACCGGGGAAGAACTGACTACCCGTAAAGACGAt                                                                     <  4:241852/135‑1 (MQ=255)
                                                             ccGAACTGTTTGTTGACCTTATCGTTGGCCGCCGCGTTCACGCGCCGTCTGGGCGTGTTTATCACTTTAAATTAAATCCGCCTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                     <  2:967051/139‑1 (MQ=255)
                                                             actaaCTGATTGTTGACCGTATCGTAGGCCGCCGGGTTCACGCGCGGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                     <  7:238783/137‑1 (MQ=255)
                                                             aCGAATTTATGGTGGACCGTATCGTAGGCCCCCGCTTTAAGGCGCCGGCTGGTCGTGTTTATCACTTTAAATACATACCGCCGAAATTATCAGGCAAAGACGACGTG‑ACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                     <  7:892725/140‑1 (MQ=255)
                                                             aCGAACTGATTGTTGACCGTATCTTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAATTATAAGGCAATGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGAt                                                                     <  2:99894/140‑1 (MQ=255)
                                                                 aCTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCAAGCGCCGTCTGGTCGTGTTTATAAAGTTAAATTAAATCCGCCGAAAGTAGAATGCAAAGAAGACGTT‑TCCGGTGAATACATGTCTTCACGTAAAAAAGATCAgg                                                                 >  3:938375/1‑140 (MQ=255)
                                                                    gATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATGACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAAACGACGTT‑ACCGGGGAAGCACTGACTACCCGTAAAGACGATCAGGAag                                                              >  4:678036/1‑140 (MQ=255)
                                                                     tttgttgACCTTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGGCGTGTTTCTCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAaga                                                             <  3:26621/139‑1 (MQ=255)
                                                                       tgttgACCGTATCGGTGGCCGCCGCGTTCACGCGCCGTCTGGTCGTTTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAgaga                                                           <  4:389771/140‑1 (MQ=255)
                                                                        gttgACCGTAGCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGAc                                                          <  2:680023/140‑1 (MQ=255)
                                                                          tgACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTCACGTTTAATTCGATCCGCCGAAATTAGAAGGCAAAGACCCCGTT‑ACCGGTGTAGAAAAGACTACCCGGAAAGACGATCAGGTAGAGAccc                                                        >  1:904865/1‑139 (MQ=255)
                                                                           tcccGTATCGTAGGCCGCCGCGTTCACGCGCCGTCGGGTCGTGTTTATCACTTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACcgt                                                       <  2:706111/138‑1 (MQ=255)
                                                                                   cGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTAACGTTAAATTAAATACGCCGAAAGTATAAGGGAAAGACGACGTT‑TACGGTGCATAACTGACTACCCGTAAAGACGCTCAGGAAGAGACCGTACGTaaaa                                               >  8:433589/1‑139 (MQ=255)
                                                                                      tgtccgccgCTTTAACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGCCAAACACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                            <  2:251101/137‑1 (MQ=255)
                                                                                      tggCCGCCGCGTGCACGCGCCGTCTGGTCGTTTTTATCACGTTAAATTCAATCCGCCGAAAGTCGAAGGCAACCACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                            <  3:567287/139‑1 (MQ=255)
                                                                                      agccggccgcGTTCACGCGCGGTGTGGTGGTGTTTCTAAGTTTAATTTCATTCCGCCGACTGTCGATGCCAACGACGACGTT‑ACCGGTGAAGATCTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                            <  4:875865/135‑1 (MQ=255)
                                                                                      aGGCCGCGGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTc                                            <  6:750144/140‑1 (MQ=255)
                                                                                         ccgccgCGTTACCGCGCCTTGTGGTCTGTTTTATCAGTTTAAATTAAATCCGCCTAACGTAGACGCCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTgg                                         <  5:771695/140‑1 (MQ=255)
                                                                                            ccgcGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑TCCGGTGAAGAACTGAATAACCGTAAAGACGATAACGAATAGACCGTAAGTATAAGTCTgggtt                                      >  4:897095/1‑137 (MQ=255)
                                                                                             cgcgTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAACGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGa                                     <  1:549657/140‑1 (MQ=255)
                                                                                             cgcgTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTgg                                     >  4:796638/1‑139 (MQ=255)
                                                                                             cgcgTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGa                                     <  5:404836/140‑1 (MQ=255)
                                                                                             cgcgTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAACTAGAAGGCAAAGCAGACCTT‑ACCGGTGAAGAACTGACTACCCCTAAAGACGAACAGGAATAGACCGTACGTAAACCTATGGGTGa                                     >  2:1054735/1‑140 (MQ=255)
                                                                                               ggttacagcggcgTGTGGGTGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGAAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                   <  4:190000/133‑1 (MQ=255)
                                                                                               ggTTCACGCGCCGTCTGGTGGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTCTAAGGCAAGGACGACGTT‑ACCGGCGAAGAACTGACTACCCGTAACGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                   <  8:608177/139‑1 (MQ=255)
                                                                                               cgTTCCCGCGCCGTCTGTCCTTTTTTATCACGTTAAATACAATCCGCCGAAATTATAACGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                   <  5:477473/140‑1 (MQ=255)
                                                                                               cgTTCACGCGCGGGCTGGTCGTTTTTATCACGTTAAATTCAAGCCGCCGAAAGAAGAAGGAAACGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                   <  6:872047/140‑1 (MQ=255)
                                                                                                 cgcACGCGCCGTCTGGTCGTGTTTATCACGTTAAATCCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATAc                                 <  1:256173/138‑1 (MQ=255)
                                                                                                    aCGCGCCGGGTGGTCGTTTTTATCACGTTAAATTCAATCCGCCGAATGTCGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCAt                              <  2:113036/140‑1 (MQ=255)
                                                                                                      gggcCGTATGGTCGTTTTAAACACTTTAAATACAATCCGCATAAATTAGACGCCAAAGACGACGTT‑ACCGGTGAATAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCa                            <  3:928939/138‑1 (MQ=255)
                                                                                                      gggcCGTATGGTCGGGTGTATCACGTTAAATTCAATCCGCCGAAAGTATAAGGCAAAGACGACGTT‑ACCGGTCAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCa                            <  5:518042/138‑1 (MQ=255)
                                                                                                      gcgcCGTCTGGTGGTTGTTATCATGTTAAATTCAATCCGCCGAACGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCa                            <  4:316758/140‑1 (MQ=255)
                                                                                                      gcgcCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGACGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCa                            <  2:1029374/140‑1 (MQ=255)
                                                                                                      gcgcCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCa                            <  3:31169/140‑1 (MQ=255)
                                                                                                      gcgcCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCTAATGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCa                            <  1:5506/140‑1 (MQ=255)
                                                                                                         ccGTCTGGTCGTGTTTTTTACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGGGAAGACCAGACTACCCGGAAAGACGATCAGGAATAGACCGTACGTAAAAGAATGGTTTAATACCAACAGaa                         >  8:916279/1‑139 (MQ=255)
                                                                                                         ccGTCTGGTCGTGTTTAGCACGTAAAATACATTCCTCCGAAATTAGAAGGCAAAGACGACGTT‑ACCGGGGAAGAACTGACTACCCGTAAAGACGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                         <  8:825707/140‑1 (MQ=255)
                                                                                                         ccGTCGGGTGGTGTTTCTCACGTTAAATTAAATCCGCCGAAAGTAGACGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                         <  4:898890/140‑1 (MQ=255)
                                                                                                           tagtGGTCGGGTTTATCCCGTTAAATTCAATCCGCCGAACGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGa                       <  6:507191/137‑1 (MQ=255)
                                                                                                              tGGTCGTGTTTATCACGTTAAATCCAATCCGCCGAAAGTCGCAGCCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTg                    <  5:766487/140‑1 (MQ=255)
                                                                                                              tGGTCGTGTTTATAATTTTTAATTCAATCGGCCTAATGTAGAAGGAAATGACGTCGTT‑ACCGGTGAAGTACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTg                    <  4:26826/140‑1 (MQ=255)
                                                                                                              ggggcGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGTCAACGACGACGTG‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTg                    <  8:665142/136‑1 (MQ=255)
                                                                                                                      gttATCACGTTAAATTCAAACCGCCGAAAGTATAAGGCAAAGACGACGTT‑AACGGTGAAGAACTTACTACCCTTAAAGACGATCAGGAAGATACCATACGTAAAAGTCTGGTGTAATACAAACAGAAGACTGCAACGCTg            >  4:648146/2‑140 (MQ=255)
                                                                                                                           cACGTTTAATTCAATCCGCCGAAAGTATAAGGGAATGACGACGTT‑ACAGGTGAAGAACAGACTACCCGTAAAGACTATCAGGAATAGACCGATCTTAAACTTCTGGTTGCATTACATCAGATGACTGCCCCGCTGCTctg       >  6:975387/1‑138 (MQ=255)
                                                                                                                           cACGTTAAATTAAATAATCCGAAAGTAGAATGCAAAGCAGACGTT‑TCCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGAACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGATGATCgg       >  6:82319/1‑140 (MQ=255)
                                                                                                                              tttAAATTCAATCCGCCGAACGTAGAAGCCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGcta    <  7:369585/139‑1 (MQ=255)
                                                                                                                               ttAATTTCAATCCGCCGAAAGTAGACGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGctac   <  7:456776/140‑1 (MQ=255)
                                                                                                                               ttAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGctac   >  6:80741/1‑140 (MQ=255)
                                                                                                                               ttAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGctac   <  2:233447/140‑1 (MQ=255)
                                                                                                                               taaaaTTCAAACCCCCGAACGGACAAGCAAAACACGACGTT‑ACCGGTGAAGAACAGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGctac   <  7:169532/138‑1 (MQ=255)
                                                                                                                                tAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGctact  >  1:309774/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTT‑ACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTACT  >  NZ_CP022686/4056828‑4057095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A