Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F2 I0 R1 193 6708.0 8620653 98.5% 8491343 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,380 T→G 16.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3800TG16.7% 282.6 / 10.8 184L229R (CTC→CGC) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (8/136);  new base G (29/5);  total (43/141)
Fisher's exact test for biased strand distribution p-value = 4.43e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCAC  >  NZ_CP022686/3734241‑3734514
                                                                                                                                             |                                                                                                                                        
ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGCAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑t                                                                                                                                          <  4:87628/140‑1 (MQ=255)
     ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGAtt                                                                                                                                     <  3:149756/140‑1 (MQ=255)
       tCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGAtttt                                                                                                                                   <  3:344577/140‑1 (MQ=255)
         aTTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGCGTTTGGCCCCCCCC‑ACGAATTTGt                                                                                                                                 >  4:798959/1‑140 (MQ=255)
          ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGtt                                                                                                                                <  2:450416/140‑1 (MQ=255)
           tATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGGCGGCCGC‑GCGATATTGttt                                                                                                                               >  2:754272/1‑140 (MQ=255)
            aTGGCGGGAGATTTCACTGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGtttt                                                                                                                              <  1:554654/140‑1 (MQ=255)
            aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGtttt                                                                                                                              <  1:544505/140‑1 (MQ=255)
            aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGtttt                                                                                                                              <  7:529719/140‑1 (MQ=255)
                cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCCC‑ACGCTTTTGTTTTAT‑A‑c                                                                                                                        >  2:257316/1‑140 (MQ=255)
                cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGGTGGCCGCCCGC‑GCGAGTTTGttttattc                                                                                                                          >  6:111285/1‑136 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGGTTGGCC‑CCCGCGCCGTTTTTGttttttcc                                                                                                                          >  1:987148/1‑136 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGCCGCCCGC‑GGGAAttttttttgaaaa                                                                                                                         >  1:813579/1‑135 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAATTGTCGCTGCGCAAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGTTTGGCCGCCCGC‑GCGATTTTGTTTTAT‑A‑CCg                                                                                                                      <  4:1078701/140‑1 (MQ=255)
                   gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCCGC‑GGGCTTTTGTTTtat‑aaaag                                                                                                                      >  1:290705/1‑136 (MQ=255)
                         tCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGGCGCCCGC‑GCGGTTTTTTTTTTT‑A‑ACGTGGtgtg                                                                                                               >  4:180646/1‑140 (MQ=255)
                         tCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTg                                                                                                               <  2:290776/140‑1 (MQ=255)
                          cAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGCGGTTGGGCCCCCCC‑GGCGTTTTGTTTTTT‑A‑ACGAGGtatta                                                                                                              >  3:627475/1‑136 (MQ=255)
                                    tgCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAATTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTTGTCTGAACGTCAGCGa                                                                                                    <  8:595993/139‑1 (MQ=255)
                                    tgCCGTAGAAGGATGAAAGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    <  3:798959/139‑1 (MQ=255)
                                    cgCCGTAGAAGGATGAACGCGTTATATAGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    <  3:41744/139‑1 (MQ=255)
                                    aGCCGTAGTAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    <  5:169268/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    <  3:124687/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    <  5:744135/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    <  6:55619/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    <  8:215738/140‑1 (MQ=255)
                                      ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAg                                                                                                  <  1:1076276/140‑1 (MQ=255)
                                      ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAg                                                                                                  <  1:1076278/140‑1 (MQ=255)
                                      ccGTAGAAGGATGAACGCGTTATATCGCTATGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAg                                                                                                  <  3:704873/140‑1 (MQ=255)
                                       cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGc                                                                                                 <  2:660353/140‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGTGTGGCCCCCCCC‑GGGATTTTTTTTTAT‑T‑CCCTGGTCTGCACGTCCGCGCAGcgg                                                                                               >  7:157838/1‑138 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCtt                                                                                               <  4:1053680/140‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGGCGCCCGC‑GCGATTTTGTTTTGT‑A‑CCGTGGTGTGAACGTCAGCGCAGCtt                                                                                               >  4:40536/1‑140 (MQ=255)
                                         tAGAAGGATGAACGCGTAATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCtt                                                                                               <  2:157095/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGTGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                          <  7:365266/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                          <  8:385911/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                          <  3:83323/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                          <  5:296940/140‑1 (MQ=255)
                                                 tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTTGGCGCCCGC‑GCGCTTTTGTTTTGT‑A‑ACGTGGTCTGAACGTAAGCGCAGCGTGTGTTCTg                                                                                       >  1:13862/1‑140 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGTTTGGGCGCCCGC‑GCGATTTTGTTTTAT‑A‑CCGGGGTGTGAACGTCAGCGCAGCGTCTGttatggg                                                                                     >  3:88067/1‑134 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTTGGCGCCCGC‑TGGATTTTTTTTTGT‑A‑ACGTGGTCTGTAAGTGAGCGAAGCATgtgttattgt                                                                                     >  7:71067/1‑134 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCCGC‑GCGGGTTTGTTTTAT‑T‑CCGTGGTCTGCAACTCAGCGCAGCGTATGTACTggg                                                                                     >  1:47110/1‑139 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTGTGGCCGCCCGC‑GCGATTTTTTTTTAT‑T‑CCGTGGTCGTAACGTAAGCGAAACGTCTGTACTGGt                                                                                     >  7:66247/1‑140 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGC‑GCGATTTTGTTTTAT‑A‑ACGTGGTCTGAAAGTAAGCGAAGCTTATGTACAGGt                                                                                     >  2:587782/1‑140 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGC‑ACGAATTTTTTTTCG‑A‑CCGTGGTCTGAAACGAAGCGAAGCATCTGtgatggg                                                                                     >  4:629417/1‑134 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCCC‑GCGCTTTTGTTTTCT‑A‑CCCGGGTGGGAAAGTCCGCGCAGCTTCGGTACTggg                                                                                     >  8:267977/1‑139 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGGTGGCCGCCCGC‑GCGATTTTTTTTTCT‑T‑CCGGGGGGTGAACGGCAGCGAAGCGTGTGTTCTGGt                                                                                     >  4:1068841/1‑140 (MQ=255)
                                                     cgcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGGGGTCTGAACGTCAGCGCAGCGTGTGTAAGGGTTa                                                                                   >  8:341457/1‑140 (MQ=255)
                                                        gTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGCGTTTGGCCGCCCGC‑GCGATTTTGTTTTGT‑G‑CCGTGGGGTTCACGTAAGCGAAGAGTCTGGACGGGTTAcgg                                                                                >  8:261861/1‑139 (MQ=255)
                                                           atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                             <  1:466452/140‑1 (MQ=255)
                                                           atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                             <  8:447542/140‑1 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGTTTGGGCGCCCGC‑GGGCATTTTTTTTGT‑A‑ACGGGGTCGGGACGGTAGCGAAGGTGGTTTACGGGTTGAgcgcgcgtgcc                                                                       >  4:1020845/1‑135 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTGT‑A‑CCGGGGTGTGAACGTCAGCGCAGCGTCTGTACTGGTTTCGCGCGCTTccc                                                                       >  4:261773/1‑139 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGC‑GAGATTTTTTTTTATAA‑CCGGGTAGTGAA‑GTCAGCGAAGCATCTGTACTTGGTACGCCCGCGTCCg                                                                       >  8:299959/1‑140 (MQ=255)
                                                                  tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGa                                                                      <  4:483694/140‑1 (MQ=255)
                                                                   ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                                     <  8:561050/140‑1 (MQ=255)
                                                                    tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGCCGGCCGC‑GCGAAGTTGTTTTAT‑AGCCGTGG‑GGGACCGTCAGCGCAGCATGTGTAAAGGTTACGCCCGCGTcccgtg                                                                    >  7:82189/1‑136 (MQ=255)
                                                                       gagggAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCATCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                                 <  1:637136/138‑1 (MQ=255)
                                                                              aTGTCGCTGATCCATGTGCGTTTTAATTATGATCAGCCCCGTC‑GGGCGTTTTGCCGACCGC‑TCGATTTTTTTTTCA‑A‑CCGTGGTCTGAAAGTCAGCGAAGCTTCTGTACTGGTTACGCGCGATTCCGATAACAATCAGCt                                                          <  8:822769/140‑1 (MQ=255)
                                                                                gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                                        <  4:9451/140‑1 (MQ=255)
                                                                                gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGAGCTTCCGATCACAATCCGCTAc                                                        <  2:654650/140‑1 (MQ=255)
                                                                                gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑GCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                                        <  3:103634/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                              <  2:96111/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                              <  4:694113/140‑1 (MQ=255)
                                                                                              tGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                          <  4:391283/140‑1 (MQ=255)
                                                                                              tGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCAGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTGAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                          <  6:680551/140‑1 (MQ=255)
                                                                                              ggCGTTTTACTGAAGATCAGCTCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                          <  4:997165/139‑1 (MQ=255)
                                                                                                      aCTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCaa                                  <  4:565657/140‑1 (MQ=255)
                                                                                                        tgatgaTCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCTGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCTAGc                                <  5:791260/140‑1 (MQ=255)
                                                                                                        tgatgaTCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                                <  5:642330/140‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                              <  2:273457/140‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                              <  3:522639/140‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGGC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                              <  5:269449/140‑1 (MQ=255)
                                                                                                           tgatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                             <  6:792496/140‑1 (MQ=255)
                                                                                                           tgatCAGCGACGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                             <  2:896404/140‑1 (MQ=255)
                                                                                                              gcAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGACTTCAGTCCCGGCTAGCCTGata                          <  1:839414/139‑1 (MQ=255)
                                                                                                               cAGCGCCGTC‑GGACGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGa                                                                                                    >  6:494412/1‑65 (MQ=255)
                                                                                                                cgcgcCGTC‑GTGCGTTTGGCCGCCCGC‑TCGATTGTGATTTCT‑T‑CCGTGGTCTGAACGTCCGCGAAGCTTCGGTACTGGTTACGCGCGCTTCCGATCACAAACCGCTAATCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  4:79338/139‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  2:862407/140‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGGTTGGCCGACCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  6:901694/140‑1 (MQ=255)
                                                                                                                 gcgcCGTC‑GGGCGGTTGGCCGCCCGC‑TCGATTTTGTTGTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGGTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaat                       <  6:628535/140‑1 (MQ=255)
                                                                                                                   gcCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                     <  4:9169/140‑1 (MQ=255)
                                                                                                                    ccGTC‑GGGCGTTTGGCCGCCCTC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGATGAATTCAGTCCCGGCAAGCCTGATAAATAAg                    <  4:801880/140‑1 (MQ=255)
                                                                                                                    ccGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                    <  4:806614/140‑1 (MQ=255)
                                                                                                                    ccGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                    <  4:543220/140‑1 (MQ=255)
                                                                                                                    ccGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                    <  5:929755/140‑1 (MQ=255)
                                                                                                                    ccGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                    <  5:839136/140‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc             <  5:909457/140‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTGACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc             <  5:12567/140‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGGCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc             <  6:177762/140‑1 (MQ=255)
                                                                                                                              gTTTGGCCGCCCGC‑GCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAg           <  6:456456/140‑1 (MQ=255)
                                                                                                                                 tGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc        <  5:482338/140‑1 (MQ=255)
                                                                                                                                 tGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc        <  1:507410/140‑1 (MQ=255)
                                                                                                                                 tGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc        <  6:652485/140‑1 (MQ=255)
                                                                                                                                 tGGCCGCCCGC‑TCGATTTTGTGTTCA‑C‑CCGTGGTCTGAACGTCCGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc        <  8:141439/140‑1 (MQ=255)
                                                                                                                                      gcccgc‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca   <  5:427562/140‑1 (MQ=255)
                                                                                                                                      gcccgc‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca   <  8:66247/140‑1 (MQ=255)
                                                                                                                                      gcccgc‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca   <  8:71067/140‑1 (MQ=255)
                                                                                                                                      gcccgc‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACATCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca   <  4:234456/140‑1 (MQ=255)
                                                                                                                                       cccgc‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCATGTCTGCcac  >  4:725218/1‑140 (MQ=255)
                                                                                                                                             |                                                                                                                                        
GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGC‑TCGATTTTGTTTTCT‑A‑CCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCAC  >  NZ_CP022686/3734241‑3734514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A