Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F12 I0 R1 158 5737.7 7407908 98.3% 7281973 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,380 T→G 13.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3800TG13.7% 229.0 / 2.1 130L229R (CTC→CGC) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (13/94);  new base G (19/2);  total (34/96)
Fisher's exact test for biased strand distribution p-value = 2.61e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGG  >  NZ_CP022686/3734245‑3734518
                                                                                                                                        |                                                                                                                                           
cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGGGTTTTGGCGCCCGCTCGag                                                                                                                                         >  3:514182/1‑139 (MQ=255)
cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGGGGTTGTCCGCCCCCACGag                                                                                                                                         >  1:255938/1‑139 (MQ=255)
      ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGtt                                                                                                                                   <  5:271257/140‑1 (MQ=255)
      ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGtt                                                                                                                                   <  1:495081/140‑1 (MQ=255)
        aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGtttt                                                                                                                                 <  2:19709/140‑1 (MQ=255)
           gCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCCGGGGGTTTGGCC‑CCCGCGCGATttttttttgtt                                                                                                                              >  4:429056/1‑137 (MQ=255)
             gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCTCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTa                                                                                                                              <  7:837917/138‑1 (MQ=255)
             gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                                            <  3:934855/140‑1 (MQ=255)
              ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGGGTGTTGGCGCCCGCGCGGTttttttttgttc                                                                                                                             >  7:479020/1‑134 (MQ=255)
              ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGTTGGGCGCCCGCGCGCTTTTTTTTTTTAccc                                                                                                                           >  2:167910/1‑139 (MQ=255)
                  aTTTCAATGCCTGGAGCCGTAGACGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGt                                                                                                                       <  5:575916/140‑1 (MQ=255)
                  aTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGTCGCTGCGCAAGGTGCGTTTTACTGATGATCAGAGCCGT‑CGGGAGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGt                                                                                                                       <  4:22275/140‑1 (MQ=255)
                                cgCCGTAGAAGGATGACCGCGTTATATCGCTTTGCGCGGGAAATGTCGCGTCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGTTCTGAACGTCAGCGa                                                                                                         <  5:73650/139‑1 (MQ=255)
                                cgCCGTAGAAGGATCAACGCGTTATATCGCGTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGAAGATCAGCGCCGG‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  5:590934/139‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTTCTGATGATCAGCGCCGG‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                                           <  7:89922/138‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                                           <  7:250053/138‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  1:650592/140‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  4:929069/140‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  5:729029/140‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  3:42696/140‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  2:795197/140‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  6:503922/140‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  1:751804/140‑1 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGTTGGGCGCCCGCGCGATTTTGTTTTTGTACGGGGTCTGAACGTCAGCGa                                                                                                         >  2:236385/1‑140 (MQ=255)
                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGGGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  6:540759/140‑1 (MQ=255)
                                aGCCGTAGAAGAATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  2:585738/140‑1 (MQ=255)
                                   cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                                      <  4:512436/140‑1 (MQ=255)
                                     tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                                    <  4:313852/140‑1 (MQ=255)
                                     tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                                    <  3:840902/140‑1 (MQ=255)
                                     tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTGTTACTGATGATCAGCGCCGT‑CGGGCGGTTTGGCGCCCCCGCGCGTTTGTTTTATTCGGTGGGCTGATAGTCCGCCAAGcgt                                                                                                    >  6:23638/1‑138 (MQ=255)
                                     agacaagATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                                    <  3:539126/134‑1 (MQ=255)
                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTa                                                                                              <  1:500053/140‑1 (MQ=255)
                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTa                                                                                              <  2:169097/140‑1 (MQ=255)
                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTa                                                                                              <  3:387774/140‑1 (MQ=255)
                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGTTTGGCGCCCCCTCGATTTTGTTTTATACCGTGGGGTGAAAGGAAGCGAATCTT‑ATGtt                                                                                              >  5:582171/1‑139 (MQ=255)
                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTa                                                                                              <  3:173966/140‑1 (MQ=255)
                                             tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         <  3:817301/127‑1 (MQ=255)
                                             tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                         >  4:817301/1‑127 (MQ=255)
                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGGTTGGCGCCCGCGCGATTTTTTTTTTGACCGTGGTGGGGACGTAAGCGCAGCTt‑gtggtaaggt                                                                                         >  4:913330/1‑133 (MQ=255)
                                                   cgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcg                                                                                     <  5:910108/140‑1 (MQ=255)
                                                   cgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGG‑CGGGCGTTTGGCCGCCCGCTCGAGTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcg                                                                                     <  5:910114/140‑1 (MQ=255)
                                                     ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTTGGCGGCCGCGCGCTTTTGTTTTTTTCAGTGGTCTGAACGGACGCGCACCGT‑CTGTACTGGTTAcgcg                                                                                   >  4:111712/1‑140 (MQ=255)
                                                     ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcgcg                                                                                   <  6:662715/140‑1 (MQ=255)
                                                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcgcgcg                                                                                 <  4:826260/140‑1 (MQ=255)
                                                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑AGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcgcgcg                                                                                 <  4:749424/140‑1 (MQ=255)
                                                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGAGCAGCGCCGT‑CGGTCGGTTGGCCGCCCGCGCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcgcgcg                                                                                 <  1:393887/140‑1 (MQ=255)
                                                            gCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCtt                                                                              <  7:693622/138‑1 (MQ=255)
                                                             cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGGGGGTGGCCGCCCCCGGGCATTTTTTTTGTAACGGGGTATGAACGTAAGCGAAGCTT‑AAGTACAGGGTTAgcgcgcgtgcc                                                                           >  2:384337/1‑135 (MQ=255)
                                                             cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGGCGCCCGCGCCATATTTTTTTTTAAACAGGTATGAACCTCAGAGAAGCTT‑ATGGAAAGGTTAagcgcccgtacc                                                                           >  4:238835/1‑133 (MQ=255)
                                                             cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcgcgcg                                                                                 >  4:364807/1‑134 (MQ=255)
                                                             cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTAcgcgcg                                                                                 <  3:364807/134‑1 (MQ=255)
                                                               ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGGGGTTTGCCGCCCGCGCGATTTTTTTTTATACCGGGGGCAGAAACTAAGCGCATGTT‑AGTTACTGGTTTCgcgcgcgtgcg                                                                           >  8:562153/1‑133 (MQ=255)
                                                               ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGAt                                                                         <  4:431670/140‑1 (MQ=255)
                                                                  cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGTTTGGCGCCCGCTGGGTTTTTTTTTATTACGTGGTCGGAACGTCAGCGCAGCGT‑CTGGACTGGTTTAGCGCGCTTGCGCTcac                                                                      >  6:207690/1‑140 (MQ=255)
                                                                   gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATcaca                                                                     <  5:263906/140‑1 (MQ=255)
                                                                   gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATcaca                                                                     <  4:22067/140‑1 (MQ=255)
                                                                          aTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCt                                                              <  1:4971/140‑1 (MQ=255)
                                                                            gTCGCTGCGCCAGGTGCGTTTTACTTATGATCAGCGCCGT‑CTGTCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACTCGCGCTTCCGATCACAATCCGCTAc                                                            <  4:504612/140‑1 (MQ=255)
                                                                            gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                                            <  2:371738/140‑1 (MQ=255)
                                                                              cGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGGGTTTGGCCGCCCCCTCGATTTTGTTTTCTTACCTGGGAGGCACGGAACAGAAGCTTGTTGTA‑TGGGTACGCGCGCATCCGCTCAACAACCGCTAc                                                            >  8:206857/1‑138 (MQ=255)
                                                                                 tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                                       <  2:552989/140‑1 (MQ=255)
                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCTCTCGATTTTGTTTTCTACCGTTGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                                  <  6:365728/140‑1 (MQ=255)
                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGATCGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGaa                                                    <  7:479668/138‑1 (MQ=255)
                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGaa                                                    <  8:629839/138‑1 (MQ=255)
                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCGCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACCGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                                  <  3:939528/140‑1 (MQ=255)
                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCAGACCGCTCGATATTGTTTTCTACAGTGGTATGAACGTCATCGAATCTT‑ATGTACTGGTTACGAGCGCTTCCGATCACAATCCGCTACTCGTTGCAtt                                                  <  6:662894/140‑1 (MQ=255)
                                                                                        ggTGCGTTTTACTGATGATCAGCGCCGT‑CCGGGGTTTGGACGCCCGCGAGAATTTTTTTTTGAACCTGGGCGGAACCTGACCGAAACGT‑ATGTAGTTGTTACCAGCGCGTCAGATAACAAACCGCTAAGCGTTTaaataa                                                >  4:245580/1‑136 (MQ=255)
                                                                                         gTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTTGCCGCCCGCGCGATTTTGTTTTGTACCGTGGTCTGAACGTCATCGAAGCTT‑CTGTAATGGTTTAGCGCTATTCCGTGAAAAATACGCGACGAGTTGAAttaaa                                               >  4:48632/1‑137 (MQ=255)
                                                                                          ggCGTTTTACTGATGATCAGCGCCGT‑CGGGCGGGTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                              <  6:138887/139‑1 (MQ=255)
                                                                                              ttttACTGATGATCAGCGCCGT‑CGGGCGGTTGGGCGCCCGCGCGAGTTTTTTTTGTAGCGTGGGGGGAACGTCAGCGCAGCGT‑ATGTTCTGGTTTAGCGCGCTTGCCCTCACAAACCGCGACTGGTTGTATTAAGTaccc                                          >  4:259427/1‑139 (MQ=255)
                                                                                                    tgatgaTCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                                    <  4:702160/140‑1 (MQ=255)
                                                                                                      atgagCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                                  <  6:599286/140‑1 (MQ=255)
                                                                                                       tgatCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGcc                                   <  7:304912/138‑1 (MQ=255)
                                                                                                        gatCAGCGCCGT‑CGGGCGGTTTGCCGCCCGCGCGAGTTTTTTTTGTAACCTGGGCGGGACGTCAGCGAACCGG‑CTGTTCTGGTTTCGCGGGCGTCCGCTGACCAACCGCGACCAGGTGAATTGAGTCCCCGCCAGCCGGa                                >  2:662025/1‑140 (MQ=255)
                                                                                                              cgcCGT‑CGGGGGTTTGGCCGCCCGCGCGATTTTGTTTTATAACGGGGTGGGAACGTCAGCGAAGCTT‑CTGTACTGGTTAAGCGCGCGTACCCTCACAATACGCAACTCGTTGAATTAAGGACCGGCCAGCCAGataaata                          >  6:840138/1‑140 (MQ=255)
                                                                                                               gcCGT‑CGGGCGTTTGGCCTCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                         <  6:659366/140‑1 (MQ=255)
                                                                                                               gcCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                         <  1:153458/140‑1 (MQ=255)
                                                                                                                ccGT‑CGGGCGTTTGGCCTCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑GTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACACGTTGAATTCAGTCCCGGGAAGCCTGataaatt                          >  7:105161/1‑137 (MQ=255)
                                                                                                                ccGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                        <  2:168437/140‑1 (MQ=255)
                                                                                                                ccGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                        <  2:370777/140‑1 (MQ=255)
                                                                                                                ccGT‑CGGGCGGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATGACTATCCGCTACTCGTTGTATTCAGTCCCGGCAAGCCTGataaata                          <  8:434842/138‑1 (MQ=255)
                                                                                                                 ggT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGATCGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg                       <  6:106573/139‑1 (MQ=255)
                                                                                                                     ggggCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTAt                    <  2:949971/139‑1 (MQ=255)
                                                                                                                        gCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCTAAGCTT‑ATGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc                 <  1:668500/140‑1 (MQ=255)
                                                                                                                        gCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc                 <  3:843749/140‑1 (MQ=255)
                                                                                                                        gCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc                 <  2:652517/140‑1 (MQ=255)
                                                                                                                        gCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCAGATCACAATCCGCTACTCGTGGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc                 <  4:414090/140‑1 (MQ=255)
                                                                                                                             tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc            <  1:401300/140‑1 (MQ=255)
                                                                                                                             tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc            <  2:714794/140‑1 (MQ=255)
                                                                                                                             tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc            <  5:100228/140‑1 (MQ=255)
                                                                                                                             tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGGTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc            <  5:301227/140‑1 (MQ=255)
                                                                                                                                  gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc         <  7:339283/138‑1 (MQ=255)
                                                                                                                                  gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc         <  8:479020/138‑1 (MQ=255)
                                                                                                                                  gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  2:191957/140‑1 (MQ=255)
                                                                                                                                  gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCAGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       >  5:748234/1‑140 (MQ=255)
                                                                                                                                   cccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCcac      >  2:347465/1‑140 (MQ=255)
                                                                                                                                    ccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  8:321000/138‑1 (MQ=255)
                                                                                                                                     cgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCcacac    >  3:538/1‑139 (MQ=255)
                                                                                                                                      gcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAgg   >  3:228650/1‑140 (MQ=255)
                                                                                                                                       ctctATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAggg  <  2:574307/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
CGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTT‑CTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGG  >  NZ_CP022686/3734245‑3734518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A