Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,380 T→G 17.3% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3800TG17.3% 230.0 / 10.2 154L229R (CTC→CGC) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (14/104);  new base G (26/5);  total (45/109)
Fisher's exact test for biased strand distribution p-value = 2.81e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

CCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCA  >  NZ_CP022686/3734243‑3734513
                                                                                                                                          |                                                                                                                                     
cccGGTCATTATGGCGGGAGATTTCAATGCATGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCTCGGGAAATGTCGCTGCGCCAGGTGCTTTTTACTGATTATCAGCGCCGTCGGGGA‑TTTGGACGGCCCCTCg                                                                                                                                     >  6:155935/1‑140 (MQ=255)
          aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGtt                                                                                                                             <  3:678331/138‑1 (MQ=255)
          aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCCGGGG‑GTTGGCCGCCCGCGCCAtttttgttt                                                                                                                           >  5:78942/1‑135 (MQ=255)
           tGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑GTTTGCCGCCCGGTGGAGTtttttt                                                                                                                            >  4:1018667/1‑138 (MQ=255)
           tGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑GTTGGCCGCCCGCGCGCtttttttt                                                                                                                            >  4:871086/1‑138 (MQ=255)
            ggCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCCCTCGCTTAtttttt                                                                                                                           >  3:802465/1‑138 (MQ=255)
             gCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑GTTTGCCCCCCCCGCGCTttttttttt                                                                                                                          >  4:747586/1‑137 (MQ=255)
             gCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑GTTGGGCGGCCGCGCGATttttttttgtt                                                                                                                        >  5:600173/1‑137 (MQ=255)
               gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTTTATCGCTTTGCGCTGGAAATGTCGCTGCGCCAGGTGCGTTTTACTTATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCTCTCGATTTTGTTTTCTAcc                                                                                                                      <  1:544272/140‑1 (MQ=255)
               gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                                      <  1:976623/140‑1 (MQ=255)
               gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑GTTGGCCGCCCGCGCGATTTTGTTTTGTAcc                                                                                                                      >  2:239203/1‑140 (MQ=255)
                ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTTGGCGGCCGCGCCAAtttttttttata                                                                                                                       >  4:718162/1‑134 (MQ=255)
                 gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGGCGCCCGCTGGATTTTTTTTTGTAGCgg                                                                                                                    >  8:594557/1‑139 (MQ=255)
                 gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑GTTGGGCGGCCGCGGGATTTTGTTTTCGACCgg                                                                                                                    >  5:797489/1‑139 (MQ=255)
                       tCAATGCCGGGAGCCGTAGAAGGATGAACGCGTTTTATCGCTTTGCGCGGGAAAGGTCGCTGCGCCAGGTGCGTTTTACTGTTGATCAGCGTCGTCGGGCG‑GTTGGCCGCCGGCTGGATTTTGTTTTCTACCGTGGTc                                                                                                                <  4:639953/138‑1 (MQ=255)
                         aaTGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGaa                                                                                                            <  7:715398/140‑1 (MQ=255)
                          aTGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAAc                                                                                                           <  5:817124/140‑1 (MQ=255)
                                ggAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑TTTTGGCGCCCCCTCGATTTTTTTTTGTAACCTGGGGTGAACGTcc                                                                                                       >  3:761793/1‑137 (MQ=255)
                                 gAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  5:61369/140‑1 (MQ=255)
                                  tgCCGTAGAAGGTTGAACGCGTTATATCGGTTTTCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCTGGCG‑TTTGGGCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  2:960743/139‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATCTCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCGCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  8:780900/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAACTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  6:770634/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑GGTGGGCGCCCGCGCGATTTTGTTTTTTAACGTGGGGTGTACGGCAGc                                                                                                     >  3:114203/1‑138 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                                     <  4:1066854/138‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  1:1093577/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  7:350508/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  6:543924/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  7:825661/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  8:831990/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  1:513107/140‑1 (MQ=255)
                                  aGCCGTAGAAGGATGAACGCGGTATATCGCTTTGAGCGGGAACTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCAGTCGGGCG‑TTTGGCCGCCCGCGCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                   <  5:1078066/140‑1 (MQ=255)
                                     cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCTCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGaa                                                                                                  <  4:910179/138‑1 (MQ=255)
                                     cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑GTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                                <  8:532556/140‑1 (MQ=255)
                                       tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                                <  3:304614/138‑1 (MQ=255)
                                       tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                              <  7:620415/140‑1 (MQ=255)
                                       tAGAAGGATGAACGCGTTATATCGCTGTGCGCGGGAAATGTCGCTGCGCAAGGTGCGTTTTACTGATGATCAGCGCCGTCCGGGG‑GTTGGGCGCCCGCACGATTTTTTTTTATAACGAGGTGTGGAAGTCAGcccagagt                                                                                              >  6:877195/1‑133 (MQ=255)
                                       tAGAAGGATGAACGAGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCTGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCGCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                              <  5:935310/140‑1 (MQ=255)
                                        ataaGGATGAACGCGGTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTTCGTTTTACTGATGAGCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCTAAGCt                                                                                               <  4:842269/136‑1 (MQ=255)
                                        aGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTc                                                                                             <  6:1017526/140‑1 (MQ=255)
                                         gAAGGATGAACGCGTTATATCTCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTTGGCGCCCGCACGCTTTTGTTTTCTACCGAGGGATGAACGTCACCGAAGCtt                                                                                              >  4:972458/1‑138 (MQ=255)
                                            ggATGAACGCGTTATATCTCTTTGAGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTGTTCTACGGTGGTCTTAACGTCAGCGAAGCTTCTGTa                                                                                         <  6:955641/140‑1 (MQ=255)
                                            ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                           <  3:113017/138‑1 (MQ=255)
                                            ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                         <  2:958334/140‑1 (MQ=255)
                                            ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCCGGGG‑TTTTGCCGCCACCGCCAGTTTTGTTTGTAACGTGGTATGAACGTCACCGCAACGTCTGTa                                                                                         >  6:787398/1‑140 (MQ=255)
                                            ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGGTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                         <  7:363260/140‑1 (MQ=255)
                                            ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGAGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                         <  1:452643/140‑1 (MQ=255)
                                            ggATGAACGAGTTATATCGCTTTGCGCGGGAAATGGCGCTGCTCCAGGTGCGTTTTACTGATTATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                         <  2:58054/140‑1 (MQ=255)
                                              aTGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGCTTTTTTTTTCTACCGTGGTCTGTACGTCAGCGAAGAATCTGTTCt                                                                                       >  5:580633/1‑140 (MQ=255)
                                                 aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTTTTTTCTACCGTGGTCTGAACGTCAGCGAATCTTATGTAATGGt                                                                                    >  7:512226/1‑140 (MQ=255)
                                                 aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCGCGAATTTGTTTTATTAAGGGGTGTGAAAGTCATCGCATCTTGTGGAATGGt                                                                                    >  6:301280/1‑140 (MQ=255)
                                                       ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑GTTGGCCGCCCGCGCGATTTTGTTTTGTTCCGTGGTCTGAACGTCAGCGAAGCATCTGTACAGGtttagcc                                                                              >  2:62970/1‑135 (MQ=255)
                                                       ttATATCGATTTGAGCTGGTAATGTCGATGCGCCTGGTTCGTTTTACTGATGATCAGATCCTTAGGTCG‑TTTGTCCTCCCGGTCGATTTTTGTTTCTACCTTGGTCTGAACGTCAGCGAATATTCTGTTCTTGTTActcg                                                                              <  6:992956/140‑1 (MQ=255)
                                                         atatCGCTTTGCGCGGGAAATGTCGCTGCGCCATGTGCGTTTTACTGATTCTCAGCGCCGTCGGGCG‑TTTTGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                            <  2:878523/140‑1 (MQ=255)
                                                         atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                              <  3:6802/138‑1 (MQ=255)
                                                         atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                              <  4:246067/138‑1 (MQ=255)
                                                         atatCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                            <  5:113291/140‑1 (MQ=255)
                                                         atatCGCATTGCGCGGGAAATGTCGCTGCGACAGGTGCGTTTTACTGATGATCCGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGCACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                              <  3:338026/138‑1 (MQ=255)
                                                             cGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                        <  6:381769/140‑1 (MQ=255)
                                                             cGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                        <  8:212882/140‑1 (MQ=255)
                                                               cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑TTTGGCCGCCCGCTCGTTTTTTTTTTATTCAGTGGTGAGAACCTAAGCGGCGCTTCTGTACTGGTTACgcgcgcgtgcc                                                                      >  5:898263/1‑135 (MQ=255)
                                                               cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGGAGCCCCCTCGTTTTTGTTTTATACCGTGGTCTGAACGTAAGCGAAGCTTCTGTTATGGTTTCGCGCGCTTCCg                                                                      >  8:525246/1‑140 (MQ=255)
                                                                tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGa                                                                     <  6:496472/140‑1 (MQ=255)
                                                                 ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGGTTTACTGCTGATCAGCGCCGTCGGGCG‑GTGGGGCGCCCGCGCGCGTTTTTTTTGTACCCGGGTGTGAACCTGATCGCAGCGTGTGTTCGGGTTACgcgcgcgtgcc                                                                      >  4:332214/1‑133 (MQ=255)
                                                                   gcgcGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATca                                                                  <  8:935326/140‑1 (MQ=255)
                                                                    cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGGGTTTTGGCCG‑CCGCGGGATTTTGTTTTGTTACGTGGTTGGTACGTCAGCGAAGAGTCTGTGCGGGGTACGCGCGCGTCCGATc                                                                   >  3:1034672/1‑138 (MQ=255)
                                                                     gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                                <  5:223632/140‑1 (MQ=255)
                                                                              gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCt                                                         <  3:416562/138‑1 (MQ=255)
                                                                              gTCGCTGCGCCAGGTGCGTTATACTGATGCTCAGCGCCGTCGGGCG‑TTTGTCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGAGCGCTTCCGATCACAATCCGCTAc                                                       <  6:535784/140‑1 (MQ=255)
                                                                                   tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGGG‑GTTGGGGGCCCGCGCGAGTTTTTTTTTTACCGTGGTGTGTACGTCCGCGAAGCGTGGGGTATGGTTTAGGGCGCTTGCGATAACCAACCGCTACTAGtt                                                  >  6:1024758/1‑140 (MQ=255)
                                                                                   tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                                  <  8:81032/140‑1 (MQ=255)
                                                                                        cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                             <  8:311976/140‑1 (MQ=255)
                                                                                        cAGGTGCGTTTTACTGATGATCAGCGCAGTCGTGCG‑TTTGGCCGACCGCTCTATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCTCTTCCGATCACAATCCTCTACTCGTTGAAtt                                             <  2:252745/140‑1 (MQ=255)
                                                                                                   tACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCgg                                    <  3:563999/138‑1 (MQ=255)
                                                                                                        atgatCAGCGCCGTCGGGCG‑TTTGGCCGCCCCCGGGGGTTTGTTTTATAGAGTGGTCTGTACGTCAGCGAAGGGTGTGTTGAGGTTTCGCGCGCCGGCGCTCACAAGCCGCGACAAGTTGAATTAATGCCCGGCCAGccg                             >  2:1000463/1‑139 (MQ=255)
                                                                                                              cgcgcCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACGGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata                         <  3:89194/137‑1 (MQ=255)
                                                                                                              aGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                       <  1:873932/140‑1 (MQ=255)
                                                                                                              aGCGCCGTCGGGCG‑GTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGTGTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCATTCCCGGCAAGCCTGataaa                       <  2:967255/140‑1 (MQ=255)
                                                                                                              aGCGCAGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                       <  8:407338/140‑1 (MQ=255)
                                                                                                                 gcCGTCGGGCG‑TTTGGCAGCCCGCTCGATTTTGTTTTCTAACGTGGTCAGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTGCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                    <  7:632482/140‑1 (MQ=255)
                                                                                                                  ccGTCGGGCG‑GTTGGCCGCCCGCGCGATTTTTTTTTGTACCGTGGTGTGAACGTCAGCGAAGCTTATGGACTGGTTAAGCGCGCTTCCGATCACAATCCGCTAATCGTTTAATTAAGTACCGGCAAGCCGGataaataaa                   >  8:777268/1‑139 (MQ=255)
                                                                                                                  ccGTCGGGCG‑CTTGGCCGCCCGCTCGATTTTGTGTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGGGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataatta                     <  3:907885/138‑1 (MQ=255)
                                                                                                                         gCG‑TTTGGCCGCCAGATCGATTTTGTTTTCTTCCGTGTTCAGAACGGCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAATCCTGATAAATTAGGTATGTc            <  2:71309/140‑1 (MQ=255)
                                                                                                                              ttGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGt        <  5:642112/140‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTTTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTTGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc       <  1:1099284/140‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGATGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgg         <  4:678451/138‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgg         <  3:870640/138‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgg         <  4:898682/138‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgg         <  4:423440/138‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc       <  7:28251/140‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc       <  1:802474/140‑1 (MQ=255)
                                                                                                                               tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc       <  6:186564/140‑1 (MQ=255)
                                                                                                                               tGGACGCCCGCTAGATTTTGTTTTCTACCGTGGTCTGAACGTCAGAGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc       <  6:842611/140‑1 (MQ=255)
                                                                                                                                ggCCGCCCGCTCGATTTTGGTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCt      >  1:83037/1‑140 (MQ=255)
                                                                                                                                    ggcggcTCGATTTTTTTTTCTACCGTGGGCTGAACGGCTGCGAAGGTTCTGTACTGGTTACGCGCGCTTCCGAGCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTAGGTCAGGTCTGCca  <  8:62450/136‑1 (MQ=255)
                                                                                                                                    gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCTAAGATTCTGTACTTGTTACGCGCGCTTCCGTTCACAATCCGCTACTCGTTGAATTCAGTCCCGGAAAGCCTTATAAATAAGGTATGTCAGGTCTga    <  3:177005/138‑2 (MQ=255)
                                                                                                                                    gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc    <  4:127261/138‑1 (MQ=255)
                                                                                                                                    gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca  <  6:696351/140‑1 (MQ=255)
                                                                                                                                    gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca  <  2:451086/140‑1 (MQ=255)
                                                                                                                                    gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca  <  5:158315/140‑1 (MQ=255)
                                                                                                                                    gcccgcTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca  <  6:870657/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
CCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCG‑TTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCA  >  NZ_CP022686/3734243‑3734513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A