Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,380 T→G 16.1% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3800TG16.1% 314.5 / 8.8 199L229R (CTC→CGC) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (13/148);  new base G (33/4);  total (47/152)
Fisher's exact test for biased strand distribution p-value = 1.16e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCAC  >  NZ_CP022686/3734241‑3734514
                                                                                                                                            |                                                                                                                                       
ggCCCGGTCATTATGGCGTGAGCTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGGGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCt                                                                                                                                         <  5:1206185/140‑1 (MQ=255)
ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCt                                                                                                                                         <  1:255574/140‑1 (MQ=255)
  cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGAGGATCAGCGCCGTC‑GGGGGGTTTGACGCCcgcgcg                                                                                                                                       >  8:1156838/1‑140 (MQ=255)
      gTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCcgcgcgct                                                                                                                                     >  4:1293324/1‑136 (MQ=255)
       tCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑Atttt                                                                                                                                 <  2:276172/140‑1 (MQ=255)
       tCATTATGGAGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTAGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑Atttt                                                                                                                                 <  8:424868/140‑1 (MQ=255)
           tATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCCCCCCCTCG‑Atttttttt                                                                                                                             >  8:217225/1‑140 (MQ=255)
            aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGtttt                                                                                                                            <  7:21486/140‑1 (MQ=255)
                cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGCGTTTGTCCCCCCCCGAG‑TTTTTGTTTTGTAc                                                                                                                        >  2:654244/1‑140 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGATGGAGGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGGTGGCCGCCCCCGCG‑CTGTTGtgttggtac                                                                                                                       >  5:109681/1‑135 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGAGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTTGCCGCCCGCGCGAATTTTGtttatacc                                                                                                                        >  6:361967/1‑135 (MQ=255)
                 gggAGATTTCAAAGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAAAGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTAcc                                                                                                                       <  8:70091/140‑1 (MQ=255)
                 gggAGATTTAAATGTCTTGATCCGTAGAAGTATGATCGCGTTATATAGCTTTGCGCGGGAAATGTCTCTGCGCCAGGTGAGTTTTAATGATTCTCAGAGCCGTC‑GGGCGTTTGGCCGCCCTCTCG‑ATTTTGTTTTCTa                                                                                                                         <  4:1102779/138‑1 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGTTTGGGCGCCCGCGCG‑ATttttttttatta                                                                                                                        >  4:933132/1‑134 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                                      <  6:250691/140‑1 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGGC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                                      <  7:454657/140‑1 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGAGCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                                      <  6:845625/140‑1 (MQ=255)
                    agaTTTCAATGCCGGGAGCCGTAGAAGGATGAACTCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑AGTTTGTTTTCTACCGtt                                                                                                                    <  7:1005177/140‑2 (MQ=255)
                     gaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGGGCCCGCGCG‑CTGTTGTTTTTTTCCgggg                                                                                                                   >  6:1232635/1‑140 (MQ=255)
                        ttCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGGTGGCCGCCCCCGCG‑CTTTTGTTTTATAACGTTGtat                                                                                                                >  6:1219366/1‑138 (MQ=255)
                                   gAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCg                                                                                                     <  1:1004308/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATTTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTCCCGTGGTCTGAACGTCAGCGa                                                                                                    <  8:274108/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                                      <  4:893569/138‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                                      <  3:851572/138‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                                      <  3:577992/138‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                    <  1:145650/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGAGCCGTC‑GGGCGTTTTGCAGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                    <  7:1107381/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGATTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                    <  5:196269/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATAACGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTGTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTTCCGTGTTCTGATCGTCAga                                                                                                      <  4:1012618/138‑2 (MQ=255)
                                    aGCCGTAGAAGGATCAAAGAGTTATCTCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGATTTACTGATCATCAGCGCCGTC‑GGGCTTTTGGCCGCCCGCTCG‑ATTTTGATTTCTACCGTGGTCAGAACGTCACCGa                                                                                                    <  2:194294/140‑1 (MQ=255)
                                     gCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGaa                                                                                                   <  1:630950/140‑1 (MQ=255)
                                        gTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCCCCGCG‑ATTTTTTTTTCTAACGTGGGATGAACGACACCGAAGca                                                                                                >  2:1136483/1‑139 (MQ=255)
                                         tAGAAGGATGCACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGCGCG‑ATTTTGTTTTATACCGTGGTCTGAACCGCACCGAAGcat                                                                                               >  6:732910/1‑138 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGCCGCCCGGGCG‑CTTTTTTTTTTTTACGGGGTGTGAACGTCAGCGAAGcgg                                                                                               >  1:564468/1‑138 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                                 <  4:696726/138‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                               <  7:417023/140‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGCGCG‑AGTTTTTTTTATACCGTGGTCTGAACGGCAGCGAAGcgt                                                                                               >  6:351228/1‑138 (MQ=255)
                                          aGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTCCCGTGGTCTGAACGTCAGCGAAGCTTc                                                                                              <  7:1185600/140‑1 (MQ=255)
                                           gAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCGCG‑ATTTTGTTTTGTAACGTGGTGTGAACGTCAGCGAAGcgt                                                                                               >  4:774484/1‑136 (MQ=255)
                                           gAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGGGGTTGGCCGCCCGCTGG‑GTTTTTTTTTATAACCTGGTGTGGACGTCAACGCACCtt                                                                                               >  4:236598/1‑138 (MQ=255)
                                            aaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                            <  7:416261/140‑1 (MQ=255)
                                            aaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                            <  1:1090154/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                          <  5:803047/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                            <  3:113487/138‑1 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGGGGTTGGGCGCCCCCGAG‑ATTTTTTTTTATAACGGGGGAGGTACCTCAGCGCAGCTTCTGTACTGGt                                                                                     >  5:409001/1‑140 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGGCCCCCCCTCG‑CTTTTTTTTTTTACCGAGGGGTGAAAGTCAGCGACGCGTCGGTTCTGGt                                                                                     >  5:630421/1‑140 (MQ=255)
                                                     cgcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGGTGGCCGCCCGCTCG‑ATTTTTTTTTCTAACGTGGTCTGAACGTCAGCGAAGCTTATGTACTGGt                                                                                     >  4:165125/1‑138 (MQ=255)
                                                         ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcg                                                                                 <  3:774484/138‑1 (MQ=255)
                                                           atatCGCTTTGCGCGGGAAATATCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                             <  2:839661/140‑1 (MQ=255)
                                                               cGCTTTGCGCGGGAAATGCCGCTGCGCCAGGTGCGGTTCCCTGATGAGCAGCGCCGGC‑GGGCGTTTGGCCGACCGGGCG‑ATTTTGTTTTCAACCGAGGGCTGAACGTCCGCGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                         <  7:707635/140‑1 (MQ=255)
                                                                gCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACTCGCGCTTcc                                                                        <  6:779635/140‑1 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGCGCG‑AGTTTTTTTTGTAACGTGGGGTGAACGTCAGCGAAGCTTATGTTCTGGTTACgcgcgcgtgcg                                                                       >  8:308251/1‑135 (MQ=255)
                                                                  tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGTTTGGCC‑CCCCCGGG‑AATTTTTTTTTTAACCTGGTGTGGACGTCACCGAACCGTGTTTAAAGGTTATGCGCGCGTccca                                                                      >  8:46874/1‑138 (MQ=255)
                                                                    tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCCCG‑CTTTTTTTTTATACCCTGGGCTGAACGTCAGAGAAGCTTCTGTACTGGGTACGCGCGCTTCCGATc                                                                    >  2:754201/1‑140 (MQ=255)
                                                                      cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGCCGCCCCCGCG‑CTTTTTTTTTATACCGTGGTCTTAACGGCAGCGCAGCTTCTGTTCTGGTTACGCGCGCGTCCGCTcac                                                                  >  2:954422/1‑140 (MQ=255)
                                                                       gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                                 <  1:588573/140‑1 (MQ=255)
                                                                       gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATca                                                                   <  3:387544/138‑1 (MQ=255)
                                                                       gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATca                                                                   <  3:641298/138‑1 (MQ=255)
                                                                          ggAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTTGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACaa                                                                <  4:331287/138‑1 (MQ=255)
                                                                           gAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGTTTGGCCGCCCGCTCG‑GTTTTGTTTTGTACCGGGGGCTGAACGTCAGCGAAGCTTATGTACTGGTTACGCGCGCTTACGCTGACAATcc                                                             >  2:784084/1‑140 (MQ=255)
                                                                              aTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGGC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCg                                                            <  3:1249075/138‑1 (MQ=255)
                                                                                ggcGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGGC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGGTACGCGCGCTTCCGATCACAATCCGCTAc                                                        <  8:1038190/138‑1 (MQ=255)
                                                                                gTCGCTGCGCCAGGTGCGTTTTACTGATGAGCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                                        <  7:252774/140‑1 (MQ=255)
                                                                                     tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                                   <  6:409048/140‑1 (MQ=255)
                                                                                        gcCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGaa                                                <  2:1315566/140‑1 (MQ=255)
                                                                                         ccAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAt                                               <  5:590708/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑TGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACATTCCGCTACTCGTTGAAtt                                              <  8:500324/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGaa                                                <  3:1199799/138‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                              <  2:1233677/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                              <  1:736358/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGGC‑GGGCGGTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                              <  7:779392/140‑1 (MQ=255)
                                                                                              tgagTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGTTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                          <  2:496458/137‑1 (MQ=255)
                                                                                              tGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                          <  2:56439/140‑1 (MQ=255)
                                                                                                    ttACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGTTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                                    <  6:1090542/140‑1 (MQ=255)
                                                                                                    ttACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                                    <  5:1037089/140‑1 (MQ=255)
                                                                                                    ttACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                                    <  7:1256688/140‑1 (MQ=255)
                                                                                                    ttACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                                    <  7:105286/140‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                              <  2:57322/140‑1 (MQ=255)
                                                                                                           tgatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                             <  7:490550/140‑1 (MQ=255)
                                                                                                           ggatAAGCTCCGGA‑GGGCTTTTGGCCGAAAGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCTAAGCTTCTGTAATGGGTATGCGCGATTCAGATCACAATCCTCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                             <  6:531954/139‑1 (MQ=255)
                                                                                                            gatCAGCGCCGTC‑GGGCGGTTGGGCGCCCGCGCG‑ATTTTGTTTTGTACCGGGGGCTGAACGTCAGCGCAGCGTGTGTTCTGGGTTCGCGCGCTTCCGATCACAAAACCCAACGCGTTGAATTAAGTCCCGGCCAGCCGGa                            >  6:1124070/1‑140 (MQ=255)
                                                                                                                cgcgcCGGC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  2:1326959/139‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCAGAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  1:866450/140‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  6:587314/140‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  6:268523/140‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGAGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  2:1067899/140‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGAGCTTCCGATCACAATCCGCTAATCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  6:627564/140‑1 (MQ=255)
                                                                                                                  cgcCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                        <  4:488957/138‑1 (MQ=255)
                                                                                                                   gcCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                     <  2:343172/140‑1 (MQ=255)
                                                                                                                     cGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAAGTTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGatatataa                     <  3:1349679/138‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc             <  1:1296856/140‑1 (MQ=255)
                                                                                                                            gCGTGTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTTGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc             <  7:307273/140‑1 (MQ=255)
                                                                                                                                 tGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGGGACGCGCGCTTCCGATCACAATCCGCTTCTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgggc        <  2:1022385/140‑3 (MQ=255)
                                                                                                                                  ggCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCTCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCt       >  5:1223629/1‑140 (MQ=255)
                                                                                                                                      gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGCAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCTGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc     <  4:46455/138‑1 (MQ=255)
                                                                                                                                      gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca   <  8:551706/140‑1 (MQ=255)
                                                                                                                                      gcccgcTCG‑ATTTTGTTTTCTACAGTGTGCTGTAAGTAAGCGAAGCTTCTGTACTGGGTACGCGCGCTTCCGATCACAATCCGCTAATCGTTGAATGCAGTCCCGGCAAGCCTGATATATAAGGTATGTCAGGTCTGc     <  4:536054/138‑1 (MQ=255)
                                                                                                                                      gcccgcGCG‑ATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGACGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGGCTGc     <  4:428157/138‑1 (MQ=255)
                                                                                                                                       cccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCcac  >  2:135279/1‑140 (MQ=255)
                                                                                                                                        ccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca   <  3:901997/138‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                       
GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCAC  >  NZ_CP022686/3734241‑3734514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A