Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F36 I0 R1 179 8076.5 8499890 98.3% 8355391 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,380 T→G 19.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3800TG19.7% 246.6 / 9.1 158L229R (CTC→CGC) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (12/109);  new base G (32/3);  total (46/112)
Fisher's exact test for biased strand distribution p-value = 5.44e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGG  >  NZ_CP022686/3734241‑3734518
                                                                                                                                            |                                                                                                                                           
ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCt                                                                                                                                             <  5:793516/140‑1 (MQ=255)
ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCt                                                                                                                                             <  5:732009/140‑1 (MQ=255)
ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTAGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCt                                                                                                                                             <  1:911681/140‑1 (MQ=255)
 gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTc                                                                                                                                            <  5:163578/140‑1 (MQ=255)
    cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTTGGCGCCCGCGCGAt                                                                                                                                         >  3:871160/1‑140 (MQ=255)
    cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGCGCGAt                                                                                                                                         >  3:766740/1‑140 (MQ=255)
     gggcATTATGGCGTGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGGTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGAtt                                                                                                                                        <  2:888564/137‑1 (MQ=255)
     ggTCCTTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGCACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGAtt                                                                                                                                        <  5:177522/140‑1 (MQ=255)
     ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGAtt                                                                                                                                        <  1:482943/140‑1 (MQ=255)
       tCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGGCGCCCGCTGGAtttt                                                                                                                                      >  1:791794/1‑140 (MQ=255)
          ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑TGGAGTTTGGCCGCCCGCTCGATTTTg                                                                                                                                     <  8:399933/138‑1 (MQ=255)
            aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGtttt                                                                                                                                 <  5:378844/140‑1 (MQ=255)
            aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGtttt                                                                                                                                 <  5:232359/140‑1 (MQ=255)
                cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTGGGGCGGCCGCCGGGGTTTGTTTTGTAc                                                                                                                             >  4:297238/1‑140 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGGTTGCCGCCCGGGCGATATTGTTTTGTatc                                                                                                                            >  1:553361/1‑138 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                                            <  5:995017/140‑1 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                                            <  1:208872/140‑1 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                                            <  5:903132/140‑1 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGAATTTGTTTTCTAcc                                                                                                                            >  4:558428/1‑140 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCCGCTCGCTtttttttttttaag                                                                                                                           >  1:417221/1‑134 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTAATGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                                           <  1:954762/140‑1 (MQ=255)
                    agaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTg                                                                                                                         <  4:114379/140‑1 (MQ=255)
                      aTTTGAATGCCTTGATCAGTAGAAGGATGAACGAGTTATATGGCTTTGCGCGGGATATTTCGCTGCGCCAGGTGCGTTTTACTGATGATCTGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACggtggt                                                                                                                       <  6:792447/140‑1 (MQ=255)
                      aTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGt                                                                                                                       <  6:710325/140‑1 (MQ=255)
                        ttCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTTGGCGCCCCCGCGCTTTTTTTTTTTTGCGTGGtgg                                                                                                                     >  5:203277/1‑138 (MQ=255)
                        ttCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGTCCGCCCCCGCACTTTTTTTTTATACCGGGGTC‑t                                                                                                                    >  1:286245/1‑140 (MQ=255)
                            aTGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGa                                                                                                                  <  8:549290/138‑1 (MQ=255)
                             tGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACg                                                                                                               <  6:286038/140‑1 (MQ=255)
                               ccTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTTGGCGCCCGCGCGATTTTTTTTTGTAACGTGGGG‑TGAAcc                                                                                                               >  8:298380/1‑137 (MQ=255)
                                    acaggTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGa                                                                                                        <  3:935557/136‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGCTCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGa                                                                                                        <  1:995529/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGGCGCCCGCGCGATTTTTTTTTTTACCGTGGTG‑TGAAAGTCAGCgc                                                                                                        >  2:531355/1‑139 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGc                                                                                                          <  7:712980/138‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGc                                                                                                          <  7:701311/138‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGc                                                                                                          <  7:459725/138‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGa                                                                                                        <  1:141112/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGa                                                                                                        <  1:888622/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGa                                                                                                        <  3:765820/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGTTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGa                                                                                                        <  2:617790/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCGCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGa                                                                                                        <  3:265095/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGGTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGc                                                                                                          <  7:654028/138‑1 (MQ=255)
                                    aGCCGGAGAAGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGGCGCTGCGCCAGGTGCGTTGTACTGATGATCAGCGCCGTC‑GGGCGTGTGGCCGCCCGCTCGATTTTGTTTTCTACCGTTGTC‑TGAACGTCAGc                                                                                                          <  7:99331/138‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGGCGCCCGCGCGATTTTGTTTTTTACCCTGGTC‑TGAACGTCCGCCaaccgt                                                                                                   >  6:801052/1‑136 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCGCCCCGCTCGATTTTTTTTTATAACCTGGTCGTGAACGAC‑GCGAAGCtt                                                                                                   >  4:287432/1‑140 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAAGGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCtt                                                                                                   <  6:984094/140‑1 (MQ=255)
                                           gAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGGCGCCCGCGCGGTTTTGTTTTATAAACGGGGC‑TGAACGTCAGCGCAGcgt                                                                                                   >  8:32808/1‑136 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTa                                                                                              <  5:581989/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTa                                                                                              <  3:996256/140‑1 (MQ=255)
                                              ggAAGAACGCGTTATATCGCTTTGCGCGTGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCAGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTa                                                                                              <  2:1047022/140‑1 (MQ=255)
                                                 tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGGGTTTGGGCGCCCCCGGGAGTTTTTTTTGTTACGTGGTG‑TGTAAGTTAGCGACGCGTATGTagtt                                                                                           >  4:360721/1‑137 (MQ=255)
                                                 tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCCGCGCGCGTTTGTTTTGTAACCTTGTG‑TGGAAGGCCGCGAAGCGTCTGttggg                                                                                           >  6:2874/1‑136 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCCCCGCGATTTTTTTTTATAACGGGGTG‑TGAAAGTCAGCGCAGCTTATGTACGGGt                                                                                         >  4:1044555/1‑140 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTTGCCGCCCGCGCGATTTTTGTTTGTAACCTGGTC‑TGAACATCAGCGAAGCGTCTGTTCTGGt                                                                                         >  1:622830/1‑140 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTGGCCGCCCGCTCCATTTTGTTTTGTACCGTGGGC‑TTAAAGTCAGCGAAGCTTCAGTAcctgt                                                                                         >  4:935557/1‑136 (MQ=255)
                                                    aCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGtt                                                                                        <  1:490451/140‑1 (MQ=255)
                                                        gTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGGGGTTGGGCGCCCGCGCGGTTTTGTTTTGTAACCGGGTG‑TGAACGACAGCCCAGCGTGAGTACTGGTTAagc                                                                                    >  2:184815/1‑140 (MQ=255)
                                                         ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGCCGCCCGCGCGATTTTGTTTTATACCGGGGTC‑TGCACGTCAGCGCCGCGTATGGACGGGTTAagcc                                                                                   >  6:379758/1‑139 (MQ=255)
                                                             atCGCTTTGCGCGGGAACTGTCGCTGCGCCAGGAGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCt                                                                               <  3:959586/140‑1 (MQ=255)
                                                               cGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                             <  5:289773/140‑1 (MQ=255)
                                                                  tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTCTACTGATGATCAGCGCCGTCGGGGGGTTTGGCC‑CCCGCGCGCTTTTGTTTTATAACGTGGTA‑TGATCGTGAGCGAAGCGTCGGTTCTGGATAAGCGCGGTTCCGa                                                                          >  2:412880/1‑140 (MQ=255)
                                                                   ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                                         <  2:948865/140‑1 (MQ=255)
                                                                    tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGCCGCCCGCGCGATTTTGTTTTGTACCGGGGGG‑TGAACGTCAGCGCAGCGTCGGGACGGGTTACGCGCGCGTCCGCTc                                                                        >  5:652747/1‑140 (MQ=255)
                                                                     gcgcGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATca                                                                       <  5:391414/140‑1 (MQ=255)
                                                                      cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGGGGTTGGGCGCCCCCGCGAGTTTTTTTTAATCCGTGGGG‑TGGACGGCAGGGCAGCGTAGGGGCTGGTTTCGAGCGCGTGCGCTcac                                                                      >  5:678401/1‑140 (MQ=255)
                                                                        cgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCCTTTGGCCGCCCGCGCGATTTTGTTTTAAACCGTGGTC‑TGAAACTCACCGAAGCGAGGTTTCAGGTTACGCGCGCTGACGGTcac                                                                      >  8:453871/1‑138 (MQ=255)
                                                                                gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTTGCCGCCCGCTCGATGTTTTTTTCTAACGGGGTC‑TGAACGTCAGCCCAGCTTCTGTACTGGGTTCGCGCGCGTCCGCTCACAATccccgac                                                            >  4:87062/1‑135 (MQ=255)
                                                                                gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGGCGCCCGCGCGATTTTGTTTTATACCGTGGTC‑TGAACGTAAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCAAAATCCGCTaa                                                            >  4:809586/1‑139 (MQ=255)
                                                                                gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                                            <  3:900518/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                                  <  4:781345/140‑1 (MQ=255)
                                                                                              tGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                              <  6:96285/140‑1 (MQ=255)
                                                                                                  ttttACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCg                                          <  1:129425/140‑1 (MQ=255)
                                                                                                        ggatgatCAGCGCCGGC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCAGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCaa                                      <  7:165987/137‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                                    <  7:217037/138‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                                  <  3:419450/140‑1 (MQ=255)
                                                                                                           ggatCAGAGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑AGAACGTCAGCGAAGCTTCTTTACTGGTTACGCGCGATTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGcc                                   <  8:629225/137‑1 (MQ=255)
                                                                                                            gatCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGa                                <  2:195350/140‑1 (MQ=255)
                                                                                                                cgctccGTC‑GGGCGATTGGCCGCCCGCTCGAGTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTTAATTCAGTCCCGGCACGCCTGataaa                            <  4:605214/136‑1 (MQ=255)
                                                                                                                agagcCGTC‑GGGCGTTTGGACGCCCGCTCGATTTTGTTTTCTACCGTTGTC‑TGAACGTCATCGAAGCTTCTGTACTGTTTACGCGCTCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                            <  2:54230/137‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                            <  6:256986/140‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                            <  6:146776/140‑1 (MQ=255)
                                                                                                                aGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                            <  6:887233/140‑1 (MQ=255)
                                                                                                                   gcCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaat                           <  7:188065/138‑1 (MQ=255)
                                                                                                                    ccGTC‑GGGGGGTTGGCCGCCCGCTCGATTTTGTTTTATACCGGGGTC‑TGCACGTCAGCGAAGCTTGTGTACTGGTTACGCGCGCGTGCCCTCACACTCCGCTACTCGTGGTATTCAGTCCCGGGAAGCCAGATAAATAAg                        >  2:755389/1‑140 (MQ=255)
                                                                                                                    ccGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                        <  3:1044555/140‑1 (MQ=255)
                                                                                                                     cGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTTGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                         <  8:841294/138‑1 (MQ=255)
                                                                                                                     cGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGGGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                         <  7:463960/138‑1 (MQ=255)
                                                                                                                           ggCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTAt                    <  8:682497/138‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAATGTATGTc                 <  4:444656/140‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc                 <  4:540063/140‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGGTGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc                 <  3:833321/140‑1 (MQ=255)
                                                                                                                            gCGTTTGGCCGCCCGCTAGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTTTACTGTTTACGCGCGCTTCAGATCACAATACTCTACTCGTTGAATTAAGTCCCGGCAAGCCTGATAAATAATGTATGTc                 <  6:622126/140‑1 (MQ=255)
                                                                                                                                 tGGCCTCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc            <  6:664854/140‑1 (MQ=255)
                                                                                                                                 tGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGACCGTCAGCGAAGCTTCTGTACTGGATACTCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc            <  4:253753/140‑1 (MQ=255)
                                                                                                                                  ggCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCt           >  3:307802/1‑140 (MQ=255)
                                                                                                                                      gcccgcTCGATTTTGTTTTCTACCGTTGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  6:523735/140‑1 (MQ=255)
                                                                                                                                      gcccgcTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  3:201386/140‑1 (MQ=255)
                                                                                                                                      gcccgcTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  2:926876/140‑1 (MQ=255)
                                                                                                                                      gcccgcTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  2:708872/140‑1 (MQ=255)
                                                                                                                                      gcccgcTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  4:836083/140‑1 (MQ=255)
                                                                                                                                      gcccgcTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGGTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca       <  3:867259/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTC‑TGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGG  >  NZ_CP022686/3734241‑3734518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A