Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,380 T→G 21.5% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3800TG21.5% 221.4 / 11.3 156L229R (CTC→CGC) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (11/103);  new base G (34/4);  total (49/107)
Fisher's exact test for biased strand distribution p-value = 5.38e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAG  >  NZ_CP022686/3734241‑3734516
                                                                                                                                           |                                                                                                                                         
ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCt                                                                                                                                           <  8:396094/140‑1 (MQ=255)
  cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTTGCCGCCcgcgcg                                                                                                                                         >  6:904724/1‑140 (MQ=255)
    cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGACAGTTGCGTTTTACTGAGTATCAGCGCCGTCGGGGGTATGTGCGCCCGCTctta                                                                                                                                       >  8:755457/1‑137 (MQ=255)
    cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGCAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGTTGGCCGCCCCCGCG‑At                                                                                                                                      >  6:726659/1‑140 (MQ=255)
    cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTATGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTGGCCGCCCGGTCg                                                                                                                                         >  2:1101110/1‑138 (MQ=255)
     ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑a                                                                                                                                       <  1:351059/138‑1 (MQ=255)
     ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑Att                                                                                                                                     <  7:583666/140‑1 (MQ=255)
       tCATTATTGCGGGAGAGTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑Att                                                                                                                                     <  1:827494/138‑1 (MQ=255)
       gcATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑Att                                                                                                                                     <  1:1068445/137‑1 (MQ=255)
                cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTGGGCGCCCGCGCG‑ATttttttttgttc                                                                                                                          >  6:542643/1‑136 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGCGGGTGGCCGGCCGCGCG‑AGTTTGttttgatcc                                                                                                                         >  6:380845/1‑135 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTa                                                                                                                           <  2:861025/138‑1 (MQ=255)
                 gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTGGCCGCCCGCGCG‑CTTTTGttttgttac                                                                                                                         >  5:908859/1‑135 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGCGGTTGGCCGCCCGCGCG‑ATTTTGTTTTGTTCCg                                                                                                                        >  3:1090470/1‑140 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTTGCCGCCCGGGCG‑ATTTTTTTTTATACCg                                                                                                                        >  3:715235/1‑140 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                                        <  8:248518/140‑1 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                                        <  4:1121101/140‑1 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                                        <  5:640627/140‑1 (MQ=255)
                  ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTGGGCGCCCGCGCG‑ATTTTTTTTtataa                                                                                                                          >  2:172567/1‑137 (MQ=255)
                      aTTTCAATGCCTGGAGCCGTAGACGGCTGAACGCGGTATATCGCTTTGCGCGGGAAATGTCGCTTCGCCAGGTGCGTTTTACTGTTGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTg                                                                                                                      <  2:349470/138‑1 (MQ=255)
                               ccTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACg                                                                                                             <  2:422594/138‑1 (MQ=255)
                                cTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTTGCCGCCCGCGCG‑ATTTTGTTTTATACCGTGGTCCGACCGTcc                                                                                                          >  8:1054859/1‑139 (MQ=255)
                                 tGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCGCG‑ATTTTGTTTTATAACGTGGGGTGAACGTCaa                                                                                                         >  4:992821/1‑139 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                      <  3:449459/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                      <  8:60203/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTGTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                      <  3:404495/140‑1 (MQ=255)
                                    aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGAGCGAGGTGCGTTTTACTGATGATCAGCGACGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                                      <  6:232595/140‑1 (MQ=255)
                                     gCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGaa                                                                                                     <  7:227871/140‑1 (MQ=255)
                                       cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                                   <  4:514930/140‑1 (MQ=255)
                                       cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGGTTTACTGATGCTCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGaa                                                                                                     <  1:292349/138‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGTTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGc                                                                                                   <  2:1031944/138‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                                 <  4:968083/140‑1 (MQ=255)
                                         tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                                 <  7:187896/140‑1 (MQ=255)
                                         gaGAAGGATGAACGGGTGATATCGCTGTGAGCGGGAAATGTCGCTGCGCCAGGGGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                                 <  4:263261/139‑1 (MQ=255)
                                           gAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGACAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGTTGGCCGCCCGCGCG‑CTTTTGTTTTATCCAGGTTTCTGAACGTAAGCGAATCtt                                                                                                 >  2:669118/1‑138 (MQ=255)
                                              ggaggaACGCGATATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCGCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  8:1075577/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  6:974959/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  4:482383/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  5:932623/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  5:778802/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGGAGCTGCGCCAGGTGCGTTTTACTGCTGATCAGCGCCGTCGTGCGTGTGGCAGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  6:1107484/140‑1 (MQ=255)
                                              ggATGAACGCGTTATATCGCTTTGCGCGGGAAAGGTCGCTGCGCCTGGTGCGGTTTACTGATGAGCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  5:715803/140‑1 (MQ=255)
                                              ggATGAACGCGGTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                            <  4:266889/140‑1 (MQ=255)
                                                 tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGTTGGCCGCCCGCGCG‑ATTTTGTTTTATAAAGTGGTGGGAAAGACAGCGAAGCGTATGTaatt                                                                                         >  8:776423/1‑137 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGCGGTTGGGCGCCCCCGCG‑GTTTTTTTTTATAACGGGGTCTGAACGTGAGCGCAGCGTATGTTCTggg                                                                                       >  3:42487/1‑139 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGTTGGGCGGCCGCGCGCATTTTTTTTTTTAACGTGG‑GGGGACGTCAGCGAAGCATATGTAATGGt                                                                                       >  3:573663/1‑140 (MQ=255)
                                                   aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGGTGGCCGGCCGCGGG‑ATGTTTTTTTGTACCGTGGTGTGCACGTGAGCGAAGCTTCTGTACTggg                                                                                       >  6:485389/1‑139 (MQ=255)
                                                       cgTGATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcg                                                                                   <  6:104353/140‑1 (MQ=255)
                                                         ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                                 <  3:847794/140‑1 (MQ=255)
                                                         ttATATCGCTTTGCGCGGGAAAAGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTATGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                                 <  5:1041322/140‑1 (MQ=255)
                                                           atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                               <  5:147747/140‑1 (MQ=255)
                                                           atatCGCGTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                               <  8:855838/140‑1 (MQ=255)
                                                             atCGCTTTGCGCGGCAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCt                                                                             <  5:726659/140‑1 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGTTTTGCCGCCCGCGCG‑GTTTTTTTTTATAACGGTGTGTGATCGTCAGCCAAGCTGATTTTATGGTTAAgcgcgcgtgcg                                                                         >  4:589102/1‑135 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCGCG‑ATTTTGTTTTATACCGTGGTCTGAACGTCAGCGAAGCTTATGTTCTGGTTACGCGCGCTTCCg                                                                         >  4:72507/1‑140 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTTGACGCCCGCGCG‑GATTTTTTTTCTACCGGGGGCTGAAAGGCAGCGCAGCGTCTGTTAAGGTTACGCCCGCATCCg                                                                         >  7:905709/1‑140 (MQ=255)
                                                                 cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTGGCCGCCCGCGCG‑CTTTTTTTTTCTTCCGTGGGCTGAACCTCAGCGCACCGTCTGTACTGGTTACgcgcgcgtacg                                                                         >  6:497902/1‑135 (MQ=255)
                                                                  tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGC‑‑CCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATc                                                                      >  3:881898/1‑140 (MQ=255)
                                                                   ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                                       <  5:598838/140‑1 (MQ=255)
                                                                    tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGTTGGCCGCCCGCTGG‑ATTTTGTTTTCTACCGTGGTCTGGACGTCAGCGAAGCGTCTGTTATGGTTACGCGCGCtgacgctc                                                                      >  4:560813/1‑133 (MQ=255)
                                                                    tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGGAGGCCGCGGG‑AGTGTGTTTTATTAAGTGGTCTGAACGTCCGCGCAGCTTCTGTACTGGTTTAGCGCGCGTACGATc                                                                      >  4:857899/1‑140 (MQ=255)
                                                                    tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGGTGGGCGGCCGCGCG‑ATTTTGTTTTGTAACGTGGGGTGAACCTCAGCGCAGCGTCTGTAGTGGTTTCGCGCGCTTCCgcta                                                                      >  4:929523/1‑137 (MQ=255)
                                                                     gcgcGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                                       <  1:663921/138‑1 (MQ=255)
                                                                       gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                                   <  5:159819/140‑1 (MQ=255)
                                                                       gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                                   <  4:1137326/140‑1 (MQ=255)
                                                                       gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGAGCAGCGCCGTCGGGCGGTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                                   <  7:841171/140‑1 (MQ=255)
                                                                                  cGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGGGTTTGGCGCCCGGGCG‑GTTTTTTTTTGTACCGGGGTGTGAACGTCTGCGAAGCTTGTGTTATGGTTACCGGCGCTTCCGCTAACAAGACGCTaa                                                          >  2:892164/1‑137 (MQ=255)
                                                                                     tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTGGCCGCCCGCGCG‑CTTTTGTTTTATACCGTGGTCTGAACCTCCGCGCAGCTTCTGTACGGGTTAACCGCGCTTCCGATCACCCACCGCGACTCggt                                                     >  8:855220/1‑138 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                                <  4:580640/140‑1 (MQ=255)
                                                                                          cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                                <  4:904062/140‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                                <  3:254220/140‑1 (MQ=255)
                                                                                                          atgatCAGCGCCGTCGGGCGGTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGTCAAGCCt                                <  4:1007259/140‑1 (MQ=255)
                                                                                                           tgatCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                               <  4:674564/140‑1 (MQ=255)
                                                                                                            gatCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGa                              <  6:1171021/140‑1 (MQ=255)
                                                                                                            gatCAGCGCCGTCGGGCGGTTGGCCGCCCGCGCG‑CTTTTTTTTTTTACCGTGGTCTGAAAGTCAGCGCAGCGTCTGGTCTGGGTAAGCGCGCGTCCGCTCACAAAACCCGACTCGTTGAATTAAGTACCCGCCAGccc                                >  2:228816/1‑137 (MQ=255)
                                                                                                                cgcgcCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTTGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTCCTCGTTGAATTCAGTCCCGGCAAGCCTGata                            <  1:966659/137‑1 (MQ=255)
                                                                                                                aGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                          <  8:482775/140‑1 (MQ=255)
                                                                                                                aGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata                            <  2:976880/138‑1 (MQ=255)
                                                                                                                aGCGCCGTCGGGAGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata                            <  2:521287/138‑1 (MQ=255)
                                                                                                                   gcCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                       <  7:109690/140‑1 (MQ=255)
                                                                                                                    ccGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaata                        <  1:112279/138‑1 (MQ=255)
                                                                                                                    ccGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg                      <  6:217826/140‑1 (MQ=255)
                                                                                                                     cGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataatg                     <  6:988676/140‑3 (MQ=255)
                                                                                                                           gCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCTTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc               <  6:746502/140‑1 (MQ=255)
                                                                                                                           gCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACAGTGGTCTGAACGTCAGCGAAGCTTCTTTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc               <  8:905709/140‑1 (MQ=255)
                                                                                                                           gCGTTTGGACGCCCGCTCG‑ATTTTGTTGTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGGACTGTTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCATGATAAATAAGGTATGTc               <  6:362157/140‑1 (MQ=255)
                                                                                                                               ttGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGt           <  8:314916/140‑1 (MQ=255)
                                                                                                                                tGGCCGCCCGGTAG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc          <  3:894272/140‑1 (MQ=255)
                                                                                                                                tGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACACTCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgggc          <  6:431202/140‑3 (MQ=255)
                                                                                                                                     gcccgcTCG‑ATTTTGTTTTCTACCTGGGTCTGAACGTCAGAGAAGCTTCTGTACTGGTTCCGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCTGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc       <  1:1064778/138‑1 (MQ=255)
                                                                                                                                     gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc       <  2:1013440/138‑1 (MQ=255)
                                                                                                                                     gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc       <  1:1192747/138‑1 (MQ=255)
                                                                                                                                     gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca     <  3:79415/140‑1 (MQ=255)
                                                                                                                                     gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca     <  6:690609/140‑1 (MQ=255)
                                                                                                                                     gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca     <  5:744930/140‑1 (MQ=255)
                                                                                                                                     gcccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAATCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca     <  5:704867/140‑1 (MQ=255)
                                                                                                                                     gcccgcGCG‑ATTTTGTTTTCTACCGTGGTCTGAACTTCAGCGACGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca     <  8:77918/140‑1 (MQ=255)
                                                                                                                                      cccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGcc      <  2:281456/138‑1 (MQ=255)
                                                                                                                                      cccgcTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTATGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTATTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCcac    >  6:813273/1‑140 (MQ=255)
                                                                                                                                       ccgcGCG‑ATTTTGTTTTCTACCGGCGTCTGAACGTCTGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTTAATTCACTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCcaca   <  7:1046916/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAG  >  NZ_CP022686/3734241‑3734516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A