Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,368,341 T→G 14.3% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,368,3410TG14.3% 183.6 / 4.9 127D47A (GAC→GCC) ybiYglycyl‑radical enzyme activating protein
Reads supporting (aligned to +/- strand):  ref base T (46/59);  new base G (15/7);  total (61/66)
Fisher's exact test for biased strand distribution p-value = 5.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GGCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGTTGAATATTGAAAATC  >  NZ_CP022686/4368204‑4368478
                                                                                                                                         |                                                                                                                                          
ggCAGCAGTCTGTTAGCGCCGTAAAATGCTGCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTTCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCa                                                                                                                                          <  7:685401/140‑1 (MQ=255)
ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGtga                                                                                                                                          >  8:633894/1‑138 (MQ=255)
ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCa                                                                                                                                          <  1:334550/140‑1 (MQ=255)
ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCa                                                                                                                                          >  3:950694/1‑140 (MQ=255)
ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCa                                                                                                                                          >  5:610280/1‑140 (MQ=255)
ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCa                                                                                                                                          >  5:65996/1‑140 (MQ=255)
ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCACGATGAATAAGCAATCCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGtgc                                                                                                                                          >  6:14692/1‑138 (MQ=255)
cccagcagTCGGTTAGCGCCGTAATATGCTCCGGGGTTAACTTTACCCGATGAAAAACCAAACCAATCAACGCGCGCTCAATCACTTCCGCTGCGGTCTATGCGCACAGCTTACAGCCATCCAGACAGAGTCCTGGGtga                                                                                                                                          >  6:96388/3‑138 (MQ=255)
 gcagcaGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCAt                                                                                                                                         <  2:163646/140‑1 (MQ=255)
 gcagcaGTCGGTGAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCGCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCAt                                                                                                                                         <  8:335915/140‑1 (MQ=255)
  cagcagTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCAt                                                                                                                                         >  2:726619/1‑139 (MQ=255)
   agcagTCGGTTATCGACGTAAAATGCTCCGGGGTTAACTTTTCCCGATTAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAc                                                                                                                                       <  7:540808/140‑1 (MQ=255)
   agcagTCGGTTAGCGCCGTAAAATGCTCCGGGTTTAACGTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAc                                                                                                                                       <  5:199550/140‑1 (MQ=255)
   agcagTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAATCAAACAATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAc                                                                                                                                       <  3:86805/140‑1 (MQ=255)
   agcagTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCATTCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAc                                                                                                                                       <  1:633653/140‑1 (MQ=255)
     ctgTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCAGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAcac                                                                                                                                     >  6:733971/3‑139 (MQ=255)
     cagTCTGTTAGCGCCGTAAAAAGCTCCGGGCTTAACTTATCCCGATGAATAAGCAAACCATTCAACGCGAGCTCAATGACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCAAGACAGAGGCGTTGGTCATAcag                                                                                                                                     <  6:46848/140‑1 (MQ=255)
     cagTCGGTTAGCGCAGTAAAATGCTCAGGTGTTAACTTTTCCCGATTAAAAAGCAACCCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTATCTCACAGCTTAAAGCCTACCCGACAGAGTCGTGGGTCATAcag                                                                                                                                     <  7:759908/140‑1 (MQ=255)
       gTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAcagca                                                                                                                                   <  7:376255/140‑1 (MQ=255)
       gTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGAGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAcagca                                                                                                                                   <  2:662739/140‑1 (MQ=255)
          ggTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGAt                                                                                                                                >  7:101345/1‑140 (MQ=255)
           gTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATTCACCAGAta                                                                                                                               >  3:459540/1‑139 (MQ=255)
           gTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATc                                                                                                                               <  7:247403/140‑1 (MQ=255)
                          tGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGcg‑cg                                                                                                               <  2:829223/140‑1 (MQ=255)
                            gtCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCGCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGc                                                                                                             <  6:399610/139‑1 (MQ=255)
                            cTCCGGGGTTAACTTTTCCCGATGAATAAGCTAACCATTCACCGCGCGCGCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATACCCCAGCTCCGGAGTGCGGgcg‑ccgg                                                                                                             >  1:518982/1‑137 (MQ=255)
                            cTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGAATACAGCAGATGCATAGTGAGGGCG‑Cggg                                                                                                             >  4:514459/1‑139 (MQ=255)
                             tCCGGGGTTAACTTTTCCCGATGAATAAGCAACCCATTCACCGCGCGCTCAATCACTTCCGGTGCGGGCTTAGCGCACAGCTTACAGCCTTCCCGACAGAGTCGTGGGTCCAACAGCCGAACCTGCGGGGGGGCC‑CGGcg                                                                                                            >  2:486941/1‑139 (MQ=255)
                             tCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTAATACCGCAGCTACATCGTGCGGGCG‑CGGCt                                                                                                            >  8:645588/1‑140 (MQ=255)
                             tCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCGTACAGCCTTCCAGACAGAGTCGTGGGTCATACAACAGATCATGAGTGCGGGcg‑ccgcg                                                                                                            >  8:645589/1‑139 (MQ=255)
                               cGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGCCAGAGTCGTGGGTGATACAGAACATACATCCTGCGGGCG‑CGGcgtg                                                                                                          >  5:318322/1‑137 (MQ=255)
                                ggggTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTc                                                                                                         <  4:501948/140‑1 (MQ=255)
                                  ggTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCg                                                                                                       <  8:524900/140‑1 (MQ=255)
                                        cTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGTTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCt                                                                                                 <  3:766272/140‑1 (MQ=255)
                                            tCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAAACACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTACAGACAGAGTCGTGGGTGATACACCAGAACCTGCCTGACGGCG‑CGGCAGTGCGGGGTCTGaca                                                                                             >  5:465406/1‑140 (MQ=255)
                                                     aTAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTATTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCa                                                                                    <  7:416631/140‑1 (MQ=255)
                                                         ccAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGccc                                                                                <  2:190872/139‑1 (MQ=255)
                                                             aCCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATACAGCAGATACAGCGTGCGGGCG‑CGGCTTTGCGGGTTCTGACACCAGCGGCAGCCCAGcc                                                                            >  3:142225/1‑139 (MQ=255)
                                                               cATTCAACGCGCGCTCAATCACTTCCTGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGTGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGaa                                                                          <  2:559503/140‑1 (MQ=255)
                                                               cATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCCGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGaa                                                                          <  1:178043/140‑1 (MQ=255)
                                                                aTTGAACGCGCGCTCAATCCCTTCCGGTGAGGTCTTAGCGCACAGCTTACAGCCTGCCAGACAGAGTCGGGGGGCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTGCTGACACCAGCGGCAGCCCAGCGAAc                                                                         <  8:130550/140‑1 (MQ=255)
                                                                aTTCCACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAAc                                                                         <  4:704376/140‑1 (MQ=255)
                                                                aTTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAAc                                                                         <  1:272559/140‑1 (MQ=255)
                                                                 ttCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATACATCCGAACCAGCGTGCGGGCG‑CGGCGTTACGGGGTCTGGCACCCGCGGCAGCCCCGCGCAcc                                                                        >  3:848753/1‑139 (MQ=255)
                                                                     ccgcgcgCTCAATCACTTCCGGTGCGGTCTTAGCGCAGAGCTTCCAGCCTTCCAGACAGAGGCGTGGGTCATACAGCAGAGCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCt                                                                    <  8:229608/139‑1 (MQ=255)
                                                                     aCGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCAAACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCt                                                                    <  6:272495/140‑1 (MQ=255)
                                                                      cgcgcgCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAAAGCAGCTCCAGCGTGCGGGCGCCGGGTTGACGGG‑TCTGACACCAGCCGCCGCCCACCGaaacaccgt                                                                   >  1:35403/1‑133 (MQ=255)
                                                                        cgcgcTCTATCACTTCCGGTGCGGTCTTCGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTa                                                                 <  6:401226/140‑1 (MQ=255)
                                                                        cgcgcTCAATCACTTCCGGTGCGGTATTATCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTa                                                                 <  2:78618/140‑1 (MQ=255)
                                                                        cgcgcTCAATCACTTCCGGGGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTa                                                                 <  4:158327/140‑1 (MQ=255)
                                                                        cgcgcTCAATCACTTCAGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGAGAACAGCCTTTa                                                                 <  8:402798/140‑1 (MQ=255)
                                                                         gcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATACAGCAGATCCTGCGTGCGGGCG‑CCGCGTTGCGGGTGATGACACCACCGGCAGCCCACCCAACAGCCGTTaa                                                                >  7:833317/1‑140 (MQ=255)
                                                                          cgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGGATAACTGAGGTACTGCGTGCGGTCG‑CGGCGTTACGGGTTCGTCAACCAGCCGCAGCCCAGCCAACACCCGTTaat                                                               >  5:257337/1‑139 (MQ=255)
                                                                                         ggTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACCTAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCg                                                <  1:899583/140‑1 (MQ=255)
                                                                                         ggTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGAAAAACCAAATACAGCGTGAGGGCG‑CGGCTTTACGGGGTCTGACACCAGACGCCGCCCCGCGCACAGACATTAAGAAAAACACCCGTGCg                                                >  8:400225/1‑140 (MQ=255)
                                                                                         ggTGCGGTCTTAGAGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGTGCATACAGCAGATCCGGCGTGCGGGCG‑AGGCTTTCCGGGTGCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCg                                                <  8:518998/140‑1 (MQ=255)
                                                                                         ggTGCGGTATTAGCGCACAGCTTACAGAATTCCAGACAGAGTCGTGGGGCATACAAACGCACCTCGGGGCGGGCA‑CCGCATTACGGGGTATGGCACCCGCGGCAGCCGAGCGAACAGCCATTAATAAATACGaacctggg                                                >  8:400226/1‑133 (MQ=255)
                                                                                           tGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATACATCAGATCCTGCGTGCGGGCG‑CGGCGTTGCGGGTTCTGCCCCCAGCGGCAGCCCCGCGCACCGCCGTTAAGAAAAAAGACCGTggggc                                              >  1:781017/1‑139 (MQ=255)
                                                                                             cGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCCTTAAGCAGATCCGGCGGGCGGGCG‑CGGCTTTACCGGTTCTGGCACCAGCGGGCACCCAGCGAACAGCCATTAACAAATACGACCCTGCCGATa                                            >  6:702371/1‑140 (MQ=255)
                                                                                             cGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAAAGCAGATCCTGCGTGCGGGCG‑CGGGGTTACGGGTTCGTACACCACCGGCAGCCCAGAGAACAGCCTTTAAGAAATAAGCACGTGCGGAtt                                            >  6:706760/1‑139 (MQ=255)
                                                                                              ggTCTTAGCGCACAGCTTACAGCCTTCCAGAGAGAGTGGTGGGTCTTACAGCAGATCCTGCGTGCGGGCG‑CGGGTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCTTACAGCATTTAAGAAATACGACCGTGCGGATAc                                           <  6:748599/140‑1 (MQ=255)
                                                                                              ggTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATAACGCAGATACTTCGTGCGGGCG‑CGGGGTTCCGGGTTGTGACCCCAGCGGCAGCCCAGCGACCAGCCTTTAAGAAATAAGACCGTTCGGATAc                                           >  5:791495/1‑140 (MQ=255)
                                                                                               gTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATAAAGCAGATCCAGCGTGCGGGCC‑CGGCGTTCCGGGTTGTGACACCCGCCGCAGACCAGCGCACAGCCTTTAAGAAATAAGACCGTGCggtttcc                                          >  2:300210/1‑135 (MQ=255)
                                                                                               gTCTTAGAGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAcc                                          <  6:23029/140‑1 (MQ=255)
                                                                                                tCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCg                                         <  3:270457/140‑1 (MQ=255)
                                                                                                   tAGCGCACAGCTTACAGCCTTACAGACAGAGTCGTGGGTAATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCgggg                                      <  5:527818/140‑1 (MQ=255)
                                                                                                         acaGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCcatca                                <  3:505748/140‑1 (MQ=255)
                                                                                                          caGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCAGCGTGCGGGCG‑CGGCGTTGCGGGGTCTGGCACCAGCGGCAGCCCAGCGCACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGGcatcag                               >  1:595163/1‑139 (MQ=255)
                                                                                                          caGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCAGCGGGCGGGCG‑CGGGGGTGCGGGTTATGGAACCCGCGGCCGCCCCGCGCACCGCCATTTAGAAAAACGACCCGGCGGATAACGGGGCcagcct                               >  6:495661/1‑136 (MQ=255)
                                                                                                          caGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATACTGCGTGCGGGCG‑CCGCATTCCGGGGTATGCCACCCGCGCCCGCCCCGCGACCAGCCTGTAAGAAATACGCCCGTGCGGATTCCGGGGCaatcat                               >  8:136639/1‑140 (MQ=255)
                                                                                                          caGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAAAGCAGATACAGCGGGCGGGCC‑CGGGGTTACGGGGTCTGACCCCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAAACGCCCCTGCGGATACCGGGGGcatcaa                               >  4:907273/1‑139 (MQ=255)
                                                                                                          caGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTGCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCcatcat                               <  6:48748/140‑1 (MQ=255)
                                                                                                          aaGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACCGCAGATCCGGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTACGAAATACGACCGTGCGGATACCGGGGCcatcat                               <  1:832598/139‑1 (MQ=255)
                                                                                                            gCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGAAGATCCAGCGTGCGGGCG‑CGGCTTTACCGGTTATGCCAACAACGGCCGCCCAGCGCACAACCTTTAAGAAATAAGAACCGGCGGATAACCGGGCCCACATgg                             >  6:527818/1‑140 (MQ=255)
                                                                                                            gCTTACAGCCTTCCAGACAGAGTCGTGGGTAATAAAGCAGATCCTGCGTTCGGGCG‑CGGCTTTACCGGGTATGACACCAGCGGCAGCCCAGAGAACAGCCGTTTAGTAAATCTACCGGTCGGCTACCGGGGGCAGAATgg                             >  4:404124/1‑140 (MQ=255)
                                                                                                             cTTACCGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCGGCGGGCGGGCG‑CGGCTTTCCGGGTGCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATggg                            <  7:642467/140‑1 (MQ=255)
                                                                                                             cTTACAGCCTTCCAGACAGAGGAGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATggg                            <  6:506342/140‑1 (MQ=255)
                                                                                                                  cgCCTTCCAGACAGAGTCGTGGGTCATACAGCAGAGCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAg                       <  5:759380/139‑1 (MQ=255)
                                                                                                                  cgCCTTCCAGACAGAGTCGGGGGTCATACAGCAGATCCTTCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAg                       <  2:745108/139‑1 (MQ=255)
                                                                                                                  cgCCTTCCACACAGAGTCGGGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTGCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAg                       <  5:847741/139‑1 (MQ=255)
                                                                                                                  ccccTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGGG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAg                       <  3:112916/138‑1 (MQ=255)
                                                                                                                  aGCCTTCCAGACATCGTCGTGGTTCATACAGCAGATCATGCGTGCGGGCG‑GGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAg                       <  6:58691/140‑1 (MQ=255)
                                                                                                                    ccTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACTACCGTGCGGATACCGGGGCCATCATGGGGCGAGTa                     <  2:234148/140‑1 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGTGATACAGCAGATACTGCGTGCGGGCG‑CGGCGTTACGGGTTCTGACACCCTCGGCAGCCCCCCGCACAGCCGTTAAGAAATAAGACCGGGCGGATACCGGGGCCAGCTTGGGTCGAggatcg                  >  4:334186/1‑135 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGTCATACAGCAGCTACTGCGTGCGGGCG‑CGGCGTTCCGGGGTCTGACACCAGCGGCACCCCCGCGAACAGCCTTTAAGAAATACCACCGTGCGGATTCCCGGGCCCTAATGGGGCGAGTAggg                  >  4:41541/1‑138 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGTCATACAGCAGATACTGCGTGCGGGCG‑CGGGTTTACGGGTTCTGACCCCAGCGGCAGCCCCGCGCACAGCCGTTAAGAAATACGACCGTGCGGATAACGGGGCCATCATGGGTCGGGTAGCg                  >  4:553190/1‑140 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGTCAAACAGCAGCTCCTGCGTTCGGGCG‑CGGCGTTAACGGGTCTGACCCCACCGGCCGACCAGCGAACATGCATTAAGAAAAAACACCGTGCGGGTAACGGGGCCAACAGGGGGCGAGTATCg                  >  2:365113/1‑140 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGTAATACAGCAGAACCTTCGTGCGGGCG‑CGGCGTTCCCGGTTCTGCCACCAGCGGCAGCCCAGCGCACCGCCATTAACAAATAATACCCGTCGGAGACCGGGGCCCACAAGGGTGGAGtttcg                  >  2:445516/1‑136 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGGCATTCAGCAGATCCTGCGTGCGGGCG‑CGGCGTTCCGGGTTCTGACACCAGCGGCCGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCCGGGCCATCATGGGTCGAGTAGCg                  >  8:448094/1‑140 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCG‑CGGGTTTCCGGGGTCTGACACCAGCGGCAGCCCCGCGAACAGCCATTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCg                  >  2:957569/1‑140 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGGCATACAGCAGATACTGGGTGCGGGCG‑CGGCGTTCCGGGGTATGCCCCCAGCGGCAGCCCCGCGAACAGCCGTTAAGAAATAAGACCGTGCGGATACCGGGGGCATCAATGGGGGAGTAGCg                  >  2:263614/1‑140 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGGCATACAGCAGAACCTTCGTGCGGGCG‑CGGCTTTACGGGTGGTGACACCCGCGGCCGCCCAGCGAACAGCCGGTAAGAAAAAAGACCGGGCGGCTACCCGGGCCAGCATGGGTCGAGGAGCg                  >  2:388933/1‑140 (MQ=255)
                                                                                                                       tCCAGACAGAGTCGTGGGGCAAAACGCCGATACAGCGTGCGGGCG‑CGGCGTTCAGGGTTCGGCCACCCGCCGCAGCCCAGCGCCCAGCCGGTAACAAATACCACCGTGCGGATACCGGGGCCATGAAGGGTAGAGTAGCg                  >  6:140819/1‑140 (MQ=255)
                                                                                                                                agTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGTTGAATAtt         <  6:791495/140‑1 (MQ=255)
                                                                                                                                agTCGTGGGTCATAAAGCAGATACTGCGTGCGGGCG‑CGGCTTTCCGGGTTCGGATACCAGCGGCAGCCCACCGATCAGCCTTTAAGAAAAACGCACGTGCGGGTACCGGGGGCATAAAGGGTGGTGTTGCGGTTAATAtt         >  4:158638/1‑140 (MQ=255)
                                                                                                                                agTCGTGGGTAATACAGCAGATACTGCGTGCGGGAC‑CGGCTTTCCGGGTTCTGACACCCGCGGCAGCCCACCGCACAAGCATTTATAAATAACAACGGGCGGAGACCGGGGCCACAAAGGGGGGAGTATCGTTGAAGAtt         >  2:813534/1‑140 (MQ=255)
                                                                                                                                  tCGTGGGTCATACAGCAGATCCAGCGTGCGGGCG‑CGGCTTTACGGGTTATGACCCCCGCGGCCGCCCAGCGAACAGCCATTAAGAAATACGACCGGGCGGATACCGGGGGCATCATGGGTCGAGTAGCGTTTAATAttta       >  4:217862/1‑138 (MQ=255)
                                                                                                                                  tCGTGGGTCAAAAAGCAGATCCTGCGTGCGGGCG‑CGGCGTTCAGCGTTATGACACCCCCGGGCGCCCCTCGAACCACCTTTAAAAAATAACACCGTGCGGCTACCCGGGCCATCATGGGGCCAGTAGCGTTTAATAttta       >  4:419869/1‑138 (MQ=255)
                                                                                                                                    gTGGGTCATAAAGCAGATCCTGCGTGCGGGCG‑CGGCGTTACGGGGTGTGACACCACCGGCCGCCCCGCCCACACCCGTTAAGAAATACGCCCCGGCGGATACCGGGGCCAACATTGGTCGAGTAGCGGTGAATATTTaaa     >  2:627829/1‑140 (MQ=255)
                                                                                                                                       ggTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCTGCGGCAGCCAAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGTTGAATATTGAAAATc  <  4:967772/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GGCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCG‑CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGTTGAATATTGAAAATC  >  NZ_CP022686/4368204‑4368478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A