Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,368,341 T→G 14.6% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,368,3410TG14.6% 210.2 / 10.4 144D47A (GAC→GCC) ybiYglycyl‑radical enzyme activating protein
Reads supporting (aligned to +/- strand):  ref base T (47/73);  new base G (19/5);  total (66/78)
Fisher's exact test for biased strand distribution p-value = 5.34e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CGGGCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAA  >  NZ_CP022686/4368202‑4368476
                                                                                                                                           |                                                                                                                                            
cGGGCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGt                                                                                                                                              <  2:811408/140‑1 (MQ=255)
  ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCa                                                                                                                                            >  2:1006990/1‑140 (MQ=255)
  ggCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCa                                                                                                                                            <  8:218898/140‑1 (MQ=255)
   gcagcaGTCGGGTAGCGCCGTAAAATGCTCCGGGGGTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCGCAATCACTTCCGGCGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCAt                                                                                                                                           <  6:444650/140‑1 (MQ=255)
     agcagTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAc                                                                                                                                         <  6:896108/140‑1 (MQ=255)
     agcagTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAc                                                                                                                                         <  5:229039/140‑1 (MQ=255)
     agcagTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTAGGTCATAc                                                                                                                                         <  2:646477/140‑1 (MQ=255)
     agcagGCGGTTAGCGCCGTAAAATGCTCCGGGTTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCTCTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCAt                                                                                                                                           <  3:1091495/138‑1 (MQ=255)
      gcagTCGGTTAGCGCCGTAAAATGCTCAGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCAtt                                                                                                                                          >  3:303910/1‑137 (MQ=255)
       cattctgTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAc                                                                                                                                         <  3:372861/132‑1 (MQ=255)
       cagTCGTTTAGAGCAGTAAATTGATCCGTGGTAAACTTTTACCGATGAATAAGCAAACCATTCAACTATCTCTCAATCACTACCGGTGCTGTCTTATCGAACAGCTTACAGCCTACAAGACAGAGTCGTCGGGAATAcac                                                                                                                                       >  2:1138699/1‑139 (MQ=255)
         gTCGGTTCGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAcag                                                                                                                                       <  4:1091865/138‑1 (MQ=255)
         gTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAcagca                                                                                                                                     <  2:10458/140‑1 (MQ=255)
           cGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGa                                                                                                                                   <  8:214457/140‑1 (MQ=255)
            ggTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCCTACAGCAGaa                                                                                                                                  >  6:216982/1‑139 (MQ=255)
                         aaaaGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCTTTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATACCGCAGTTGCTGCGTgggg                                                                                                                       >  3:36833/1‑138 (MQ=255)
                         aaaTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCgg                                                                                                                       >  4:141698/1‑138 (MQ=255)
                           aTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACCCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGgcgc                                                                                                                   <  5:966915/140‑1 (MQ=255)
                               tCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATACAGCAGATCCTGCGTGCGGGCGCGGcg                                                                                                               >  2:425741/1‑139 (MQ=255)
                                 cGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAGTCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATAAAGCAGATCCTTCGTGCGGGCGCGGCgtt                                                                                                             >  2:66949/1‑140 (MQ=255)
                                 cGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACCGCACATCCTGCGTGCGGGCGCGGCgtt                                                                                                             >  2:961197/1‑140 (MQ=255)
                                 cGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATGCAGCGTGCGGGCGCGGcggt                                                                                                             >  1:1023235/1‑137 (MQ=255)
                                  ggggTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTc                                                                                                            <  1:39990/140‑1 (MQ=255)
                                   gggTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCCTGCGGGCGCGGCttt                                                                                                             >  3:989196/1‑138 (MQ=255)
                                        aaCTATTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCCCAACCTGCCGGCGGGCCCCGCTTTACggg                                                                                                        >  4:562820/1‑138 (MQ=255)
                                          cTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCt                                                                                                    <  2:1131134/140‑1 (MQ=255)
                                          cTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCt                                                                                                    <  7:48546/140‑1 (MQ=255)
                                                 cGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGCGTCGTGGGTCCTACAGCAGCTCCTGCGGGCGGGCGCCGCTGTCCGGGGTCTGaccc                                                                                               >  4:229962/1‑136 (MQ=255)
                                                         aaGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCa                                                                                      <  4:746252/138‑1 (MQ=255)
                                                               aCCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCATCTCCAGCGTGCGGGCGCCGCGTTACGGGGTCTGACCC‑CCGCCGAAGCCCACCg                                                                              >  7:940843/1‑140 (MQ=255)
                                                                 cATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGaa                                                                            <  5:225927/140‑1 (MQ=255)
                                                                 cATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGaa                                                                            <  5:67821/140‑1 (MQ=255)
                                                                 cATTCAACGCGCGCTCAATCACATCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCg                                                                              <  3:792764/138‑1 (MQ=255)
                                                                 cATTCAACGCGCGCTCAATCAATTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCTGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGaa                                                                            <  8:414350/140‑1 (MQ=255)
                                                                  aTTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCCCAGATTACAGCCTTCCAGACAGAGTCGTGGGTCCTTCAGCAGATCCTGCCTGCGGGCGCGGCTGAGCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAAc                                                                           >  5:333165/1‑140 (MQ=255)
                                                                  aTTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGa                                                                             >  4:583165/1‑138 (MQ=255)
                                                                    tCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACACCAGATCCTGCGTGCGGGCGCCGCGTTGCGGGTTCTGACCC‑CACCGGCAGCCCCGCGacacc                                                                         >  7:17873/1‑136 (MQ=255)
                                                                          cgcgcgCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATCCAGCAGATCCTGCGTGCGGGCGCGGCGTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTa                                                                   <  8:490501/140‑1 (MQ=255)
                                                                          cgcgcTCAATCACTTCGGGTGCGGTCTTAGCGCAGAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCGGCGTGGGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCtt                                                                     <  4:324520/138‑1 (MQ=255)
                                                                          cgcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCtt                                                                     <  3:141698/138‑1 (MQ=255)
                                                                          cgcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTa                                                                   <  1:1018151/140‑1 (MQ=255)
                                                                          cgcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGACGCTCCTGCGTGCGGGCGCGGCGTTCCGGGGTCTTCCAC‑CCGCGGCAGCCCCGCGCACAGCCTTTa                                                                   >  6:848798/1‑140 (MQ=255)
                                                                          cgcgaTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCATATCCTGCGTGCGGGCGCGGCGTTCCGGGTTCTGCCAT‑CAGCGGCAGCCCAGCGAACAGCCTTTa                                                                   <  8:994089/140‑1 (MQ=255)
                                                                                 aaTCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACATAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑a                                                           <  5:216982/140‑1 (MQ=255)
                                                                                 aaTCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑a                                                           <  8:995686/140‑1 (MQ=255)
                                                                                     aCTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACg                                                         <  4:822210/138‑1 (MQ=255)
                                                                                       ttCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCCTACAGCAGCTCCTGCGTGCGGGCGCGGCGTTGCGGGGTGTGACAC‑CCGCGGCGCGCCAGCGAACACCCGGTAATAAAT‑AAGAccc                                                     >  7:1017414/1‑139 (MQ=255)
                                                                                       ttCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATAAACCAGATACAGCGTGCCGGCGCCGCGTTCCGGGGTCTGACAC‑CCCCGGCACCCCAGCCAACAGGCATTAAAAAAT‑ACGCCCg                                                     >  5:522100/1‑140 (MQ=255)
                                                                                         ccGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTAATAAAGCATCTCCTGGGTGGGGGCGCGGCTTTACGGGTGATGAAAA‑CCTCGTCAGACCCACGAAAAGCCTTTAAGAAAT‑AAGAcactg                                                   >  6:996682/1‑136 (MQ=255)
                                                                                         ccGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCAGCGTGCGGGCGCGGCGTTCCGGGGTCTGACAC‑CACCGGCCGCCCCGCGCACAGCCATTTAGAAAT‑AAGACCGTg                                                   >  1:1074015/1‑140 (MQ=255)
                                                                                           ggTGCGGTCTTTGCGCACAGCTTAAAGACTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CTGCGGCAGCCAAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCg                                                 <  5:187776/140‑1 (MQ=255)
                                                                                           ggTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCg                                                 <  1:708011/140‑1 (MQ=255)
                                                                                             tGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CCGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGa                                               <  6:929391/140‑1 (MQ=255)
                                                                                               cGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCCGATCCTGCGTGCGGGGCCGGCGTTGCGGGTTATGACCC‑CAGCGGCAGCCCAGCGACCAGCCTTTAAGCAAT‑AAGACCGTTCggg                                               >  4:835879/1‑137 (MQ=255)
                                                                                                ggTCTTAGCGCACAGCTTACCGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTGCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑Ac                                           <  2:878740/140‑1 (MQ=255)
                                                                                                 gTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑a                                            <  3:583165/138‑1 (MQ=255)
                                                                                                 gTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCGTTCCGGGTTCTG‑CACACAGCGGCAGCCCCGCGCACCGCCTTTTATAAAA‑AAGACCGTGCGGCT‑a                                            >  3:677316/1‑138 (MQ=255)
                                                                                                 gTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGAATACACGAGATACAGCGTGGCGGCGCGGCTTTCCGGGTTATTACAC‑CACAGTGAGCCCAGCGAACACCCTTCAAGAAAT‑AAGACCGGTCGGAttac                                           >  6:68600/1‑137 (MQ=255)
                                                                                                     tAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑Cgg                                       <  3:1100704/138‑1 (MQ=255)
                                                                                                     tAGCGCACAGATTACTCCATTCCAGACAGAGTCGTGGGTCATACATCAGATCCTGCGTGGGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑Cgggg                                     <  1:739162/140‑1 (MQ=255)
                                                                                                      tgcgcACAGCTTACTGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGc                                    <  1:278751/139‑1 (MQ=255)
                                                                                                          cacaGCTTACAGCCTTCCAGACTGAGGCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCca                                  <  3:206385/138‑1 (MQ=255)
                                                                                                          cacaGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGAGCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCca                                  <  3:309869/138‑1 (MQ=255)
                                                                                                            caGCTTACAGCCTTCCTGACAGTGTCGTGGGTCATACAGCATATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCTGCCCTGCGAACAGCCTTTAAGAAAT‑ACGACGGTGCGGAT‑AC‑CGGGGCcatc‑at                             <  5:983868/140‑1 (MQ=255)
                                                                                                            caGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATAAAGCAGATCCTGCGTGCGGGCGCGGCATTGCGGGGTCTGACAC‑CCCCGGCCGCCCCGCGAACCGCCTTTAAGAAAT‑ACCGCCGGGCGGCT‑AC‑CGGGGgcagc‑aa                             >  6:736059/1‑139 (MQ=255)
                                                                                                            caGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATAAAGAAGCTACAGCGTGCGGGCGCGGCATTCCGGGGTATGCCAC‑CACCGGCAACCCAGCGGACAGCCTTTTAGAAAT‑ACGACCGGGCGGTTAAC‑CGGGGcctcat                               >  2:492722/1‑136 (MQ=255)
                                                                                                              gCTTACAGCCTTCCAGACAGAGTCGTGGGTGGTACAGCAGATCCTGCGTGCGGGCGCGGCGTTGCGGGTTCTGGGAC‑CAGCGGCCGCCCCGCGCACAGCCGGTAAGAAAA‑ACGGCCGTGGGGCT‑TCGCGGGGcctaatgg                             >  2:762378/1‑134 (MQ=255)
                                                                                                              gCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACCGCAGATACTGCGTGCGGGCGCGGCATTACGGGTTCTGACAC‑CCGCGGCAGCCCAGCGCACAGCCGTTAAGAAAT‑ACGAACGTGCGGAT‑AC‑CGGGGCcataaa                              >  4:74071/1‑135 (MQ=255)
                                                                                                              gCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTACGGGTTGTGACAC‑CAGCGGCAGCCCAGCGCACAGCCGTTAAGAAAT‑ACGCCCGTGCGGAT‑AC‑CGGGGCCATC‑AAgg                           >  8:851949/1‑140 (MQ=255)
                                                                                                              gCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATACTGCGTGCGGGCGCGGCGTTCCGGGGTCTGACCC‑CAGCGGCAGCCCAGCGCACCGCCTTTAAGAAAT‑ACGACCGGGCGGTT‑TC‑CGGGGCcctc‑at                             >  3:871032/1‑138 (MQ=255)
                                                                                                              gCTTACAGCCTTCCAGACAGAGTCGTGGGTCAAACAGCAGATCCTGCGGGCGGGCGCGGCTTTCCGGGTTCTGTCAC‑CACCGGCAGCCCAGCGAACCGCCTTTAAGAAAT‑AAGACCGTGCGGAT‑AC‑CGGGGGcatc‑aa                             >  4:795758/1‑137 (MQ=255)
                                                                                                               cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATggg                          <  1:737975/140‑1 (MQ=255)
                                                                                                               cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATggg                          <  1:16414/140‑1 (MQ=255)
                                                                                                               cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATggg                          <  1:545807/140‑1 (MQ=255)
                                                                                                               cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATg                            <  3:168568/138‑1 (MQ=255)
                                                                                                               cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATg                            <  3:272996/138‑1 (MQ=255)
                                                                                                               cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTGCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATggg                          <  5:66696/140‑1 (MQ=255)
                                                                                                               cTTAAATCCTTCCAGACAGTGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGTCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATggg                          <  8:73789/140‑1 (MQ=255)
                                                                                                                      ccTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTa                   <  2:410337/140‑1 (MQ=255)
                                                                                                                         tCCAGACAGTGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGGTTTCCGGGGTCGGACCC‑CAGCGGCCGGCCAGCGCCCCTCCTGTAAGTAAT‑ACGACCGTGCGGAT‑AC‑CGGGGGCGGC‑ATGGGGGGAGTAGCg                >  1:115098/1‑140 (MQ=255)
                                                                                                                         tCCAGACAGAGTCGTGGGTCATACAGCAGCTACTGCGTGCGGGCGCGGCGTTCCGGGGTCGGGCCC‑CAGCGGCAGCCCAGCGCACAGCCTGTAAGAAAT‑ACGCCCGTGCGGCT‑AC‑CGGGGGCATC‑ATGGGTCGCGTAGCg                >  2:264316/1‑140 (MQ=255)
                                                                                                                         tCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCGTTACCGGTTATGACAC‑CCGCGGCCGCCCCGCGAAACGCCGTTAAGAAAT‑ACGACCGTGCGGAT‑AA‑CGGGGCCATC‑ATGGGTCGCGGAGCg                >  6:924641/1‑140 (MQ=255)
                                                                                                                         tCCAGACAGAGTCGTGGGTCAGACACCAGATACTGCGTGAGGGCGCGGGTTTCAGGGTTAGGACCC‑CACCGGCAGCCCCGCGACCAGCCTTTTAGAAATAATAACCGT‑CGGAT‑AA‑CGGGGGCCTA‑AAGGGTCGAGTAACg                >  2:347463/1‑140 (MQ=255)
                                                                                                                         tCCAGACAGAGTCGTGGGTCAAACAGCAGCTACTGCGTGCGGGCCCGGCGTTCCGGGTTGTGCCAC‑CAGCGGCAGCCCACCGACCAGCCTTTAAAAAAT‑ACCCCCCTGCGGCT‑AC‑CGGGGCCAGC‑AAGGGTCGAGTAGCg                >  2:1003716/1‑140 (MQ=255)
                                                                                                                         tCCAGACAGAGTCGTGGGGCCTACAACAGATCCGGCGGGCGGGCGCGGGGTTCCGGGTTAGGGCCA‑CCGCGGCGGCCCAGCGCCCAGCCTTGTAGAAAT‑AAGCCCGTTCGGAG‑AC‑CAGGGCCATC‑TTGGGGCGAGTAgag                >  8:481208/1‑138 (MQ=255)
                                                                                                                         tCCAGACAGAGTCGTGGGGCCAAAAGCACATCCTGCGTGCGGGGCCGGCGTGACGGGGTGAGCAAC‑CCCCGGCCGCCCCCCGAAAACCCGTTAAGAAAA‑AAGACCGGGCGGAT‑TA‑CCGGGCCATCAATGGGT‑GAGTAgag                >  8:566733/1‑138 (MQ=255)
                                                                                                                         tCCAGACAGAGTCGTGGGGCATACAGCAGATACAGCGTGCGGGCGCGGCGTTCCGGGTTCTGACAC‑CCGCGGCAGCCACGCGCACAGCCCTTAAGAAAT‑ACCCCCGAGCGGAT‑AC‑CGCGGCGAGC‑AGTGGGCGTGTAGCg                >  8:318793/1‑140 (MQ=255)
                                                                                                                           cagacagaGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTACGGGGTCTGACCC‑CAGCGGCCGCCCAGCGCACAGCCTTTAAGAAAT‑ACGCCCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCg                >  4:741811/1‑138 (MQ=255)
                                                                                                                           cagacagaGTCGTGGGTCATACAGCAGATCCAGCGTGCGGGCGCGGCTTTCCGGGTTCTGACCC‑CAGCGGCCGCCCAGCGAACAGCCGTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCCTC‑ATGGGTCGAGTAGCg                >  4:973314/1‑138 (MQ=255)
                                                                                                                           cagacagaGTCGTGGGTCATACAGCAGAAACAGCGTGCGGGCGCGGCTTTCCGGGTTATGACCC‑CCGCGGCCGCCCAGCGAACAGCCAGTATGAAAA‑ACGACCGGGCGGCT‑AC‑CCGGGGCCTC‑AGGGGGCGAGTAGCg                >  4:462373/1‑138 (MQ=255)
                                                                                                                                agagTCGTGGGTCATACAGCAGAGCCTGCGTGCTGGCGCGGATTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑AGGGGTCGAGGAGCGTTGAata         <  6:203839/140‑1 (MQ=255)
                                                                                                                                  tgTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt       <  1:852021/139‑1 (MQ=255)
                                                                                                                                  tgTCGGGGGGCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACCC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt       <  7:554016/139‑1 (MQ=255)
                                                                                                                                  agTCGTGGGTGATACAGCAGATCCTGCGTGGGGGCGCGGCGTTGCGGGGTGTGACCC‑CAGCGGCCGCCCCGCGAACAGCCGTTTAGAAAT‑ACGACCGGGCGGAT‑AC‑CGGGGCCCTC‑AAGGGTCGAGTTGCGGTGAATAtt       >  6:310093/1‑140 (MQ=255)
                                                                                                                                  agTCGTGGGTCATACAGCCGCTCCGGCGGGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CCGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt       <  7:81385/140‑1 (MQ=255)
                                                                                                                                  agTCGTGGGGGCTAAACCAGATCCAGCGTGCGGGCGCGGCTTTCCGGGGTCTGGCAC‑CCGCGGCCGCCCAGCGAACAGCCTGTAAGAAAT‑ACGACCGTTGGGAT‑AC‑CGGGGCCCTC‑ATGGGTCGAGTAGCCTTGAATAtt       >  8:365708/1‑140 (MQ=255)
                                                                                                                                  agTCGTGGGGGATACAGCAGATCCTGCGTGCGGGCGCGGCGTTACGGGGTGTGCCCC‑CAGCGGCCGCCCCGCGAACAGCCGTTAAGAAAT‑AAGACCCGGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt       >  2:1021177/1‑140 (MQ=255)
                                                                                                                                  agTCGTGGGGCATAAAGCAGATACAGCGTGCGGGCGCGGCGTTACGGGGTATGACAC‑CCGCGGCCGCCCCGCCCACAGCCGTTTAGAAAT‑ACGACCGTGCGGCT‑AA‑CGGGGCCAGC‑ATTGGTAGAGTAGCGTTGTAAAtt       >  8:53877/1‑140 (MQ=255)
                                                                                                                                  agTCGAGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CTGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt       >  6:537212/1‑140 (MQ=255)
                                                                                                                                     cGTGGGGCATACAGCAGAACCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTg      <  3:1005721/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CGGGCAGCAGTCGGTTAGCGCCGTAAAATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAT‑ACGACCGTGCGGAT‑AC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAA  >  NZ_CP022686/4368202‑4368476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A