breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 194 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,819,3180GA62.1% ‑4.1 / 27.2 29intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
*NZ_CP0092734,225,9540AG61.1% ‑1.2 / 13.4 18S41G (AGT→GGT) ‡yjbEexopolysaccharide production protein YjbE
*NZ_CP009273276,8000AC60.0% 11.4 / 27.9 36Y299D (TAT→GAT) yagAIS481‑like element ISEc19 family transposase
*NZ_CP0092734,594,1610CT58.2% 0.9 / 75.1 67S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092732,380,3410GA56.4% ‑1.8 / 49.9 39intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092731,819,3130GA55.6% 3.5 / 29.4 27intergenic (‑119/+84)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
*NZ_CP0092734,594,1670CT54.5% 15.4 / 79.5 67S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092731,906,0110TG54.2% 29.5 / 55.2 59T275P (ACC→CCC) htpXprotease HtpX
*NZ_CP0092732,380,3570AT54.0% 28.6 / 54.7 37intergenic (+47/‑56)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP009273585,3860AG53.8% 13.8 / 32.0 26L342P (CTC→CCC) nfrAbacteriophage adsorption protein NfrA
*NZ_CP0092732,380,3620CT53.8% 10.6 / 56.4 39intergenic (+52/‑51)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP00927338,9430TG53.1% 11.8 / 28.6 32Y58S (TAC→TCC) caiBL‑carnitine CoA‑transferase
*NZ_CP009273711,0810AC52.9% 22.5 / 31.6 34T72P (ACC→CCC) BW25113_RS03560lipoprotein
*NZ_CP0092732,428,3610TG52.6% 5.3 / 15.4 19Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase TruA
*NZ_CP0092734,225,9560TG52.6% 5.4 / 13.1 19S41R (AGT→AGG) ‡yjbEexopolysaccharide production protein YjbE
*NZ_CP009273890,3860AC52.4% 14.6 / 17.4 22intergenic (+34/‑61)potF/potGspermidine/putrescine ABC transporter substrate‑binding protein PotF/putrescine ABC transporter ATP‑binding subunit PotG
*NZ_CP0092731,154,0680TG52.0% 12.1 / 21.3 26G248G (GGT→GGGptsGPTS glucose transporter subunit IIBC
*NZ_CP0092734,058,9580GA51.9% 25.1 / 69.5 52L57L (CTG→TTG) yihOMFS transporter
*NZ_CP009273585,3900TG51.8% 1.9 / 24.5 27T341P (ACC→CCC) nfrAbacteriophage adsorption protein NfrA
*NZ_CP0092732,738,4480AC51.8% 8.5 / 25.2 27V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD

Marginal new junction evidence (lowest skew 10 of 13 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 1203246 =39 (0.650)6 (0.120) 6/132 2.5 17.3% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 26 (0.540)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 3135029 =80 (1.340)3 (0.060) 3/128 3.4 4.5% coding (1320/1704 nt) hybC hydrogenase 2 large subunit
?NZ_CP009273 3135047 = 66 (1.420)coding (1302/1704 nt) hybC hydrogenase 2 large subunit
* ? NZ_CP009273 670004 =51 (0.850)3 (0.060) 3/136 3.5 6.4% coding (236/2583 nt) leuS leucine‑‑tRNA ligase
?NZ_CP009273 670018 = 45 (0.910)coding (222/2583 nt) leuS leucine‑‑tRNA ligase
* ? NZ_CP009273 3950787 =44 (0.740)4 (0.080) 3/144 3.6 9.5% coding (394/894 nt) ilvY HTH‑type transcriptional activator IlvY
?NZ_CP009273 3950869 = 38 (0.730)coding (312/894 nt) ilvY HTH‑type transcriptional activator IlvY
* ? NZ_CP009273 = 376908472 (1.210)4 (0.080) 3/144 3.6 5.2% coding (153/588 nt) mtlR mannitol operon repressor MtlR
?NZ_CP009273 = 3769168 82 (1.570)coding (237/588 nt) mtlR mannitol operon repressor MtlR
* ? NZ_CP009273 = 3572747110 (1.840)3 (0.060) 3/144 3.6 3.2% coding (259/696 nt) yhhW quercetin 2,3‑dioxygenase
?NZ_CP009273 = 3572830 84 (1.600)coding (176/696 nt) yhhW quercetin 2,3‑dioxygenase
* ? NZ_CP009273 = 334120270 (1.170)3 (0.060) 3/144 3.6 5.7% coding (729/855 nt) rapZ RNase adapter RapZ
?NZ_CP009273 = 3341326 38 (0.730)coding (853/855 nt)
coding (2/273 nt)
rapZ
npr
RNase adapter RapZ
PTS phosphocarrier protein NPr
* ? NZ_CP009273 = 337520657 (0.960)3 (0.060) 3/146 3.7 5.4% coding (1105/1368 nt) degQ serine endoprotease DegQ
?NZ_CP009273 = 3375218 54 (1.020)coding (1117/1368 nt) degQ serine endoprotease DegQ
* ? NZ_CP009273 = 303119335 (0.590)3 (0.060) 3/148 3.7 8.6% coding (273/1734 nt) recJ single‑stranded‑DNA‑specific exonuclease RecJ
?NZ_CP009273 = 3031201 32 (0.590)coding (265/1734 nt) recJ single‑stranded‑DNA‑specific exonuclease RecJ
* ? NZ_CP009273 1356258 =37 (0.620)3 (0.060) 3/146 3.7 8.3% coding (91/558 nt) puuR HTH‑type transcriptional regulator PuuR
?NZ_CP009273 1356308 = 33 (0.620)coding (141/558 nt) puuR HTH‑type transcriptional regulator PuuR