breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 89 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273937,1690TG56.5% ‑3.7 / 18.5 23V252G (GTG→GGG) dmsAdimethylsulfoxide reductase subunit A
*NZ_CP0092733,203,0950TC55.6% 5.5 / 12.8 18S270G (AGT→GGT) tsaDtRNA (adenosine(37)‑N6)‑threonylcarbamoyltransferase complex transferase subunit TsaD
*NZ_CP0092732,089,5710AG53.3% 9.7 / 31.1 30I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
*NZ_CP0092732,371,3140GA52.9% 3.1 / 17.7 17F475F (TTC→TTTmenD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase
*NZ_CP0092731,213,8650GT52.6% 12.1 / 25.7 19S361I (AGT→ATT) pdeGcyclic diguanylate phosphodiesterase
*NZ_CP009273701,0090TA50.0% 12.8 / 12.1 22I537N (ATC→AAC) nagEPTS N‑acetyl glucosamine transporter subunit IIABC
*NZ_CP0092734,594,1610CT50.0% 38.3 / 48.9 63S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273832,0420CG48.5% 26.9 / 11.3 33A79G (GCG→GGG) hcxBhydroxycarboxylate dehydrogenase HcXB
*NZ_CP0092732,852,4660TG48.5% 11.2 / 11.7 33V672G (GTG→GGG) mutSDNA mismatch repair protein MutS
*NZ_CP0092734,612,0670AG47.5% 29.8 / 15.7 41E161G (GAG→GGG) yjjJtype II toxin‑antitoxin system HipA family toxin YjjJ
*NZ_CP0092734,594,1670CT46.8% 52.0 / 41.2 63S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092732,380,3410GA45.2% 20.9 / 22.1 32intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092731,819,3180GA45.0% 12.9 / 12.7 20intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
*NZ_CP0092732,778,6860TG42.6% 76.5 / 10.9 69intergenic (‑316/+435)BW25113_RS25705/BW25113_RS13825DUF5507 domain‑containing protein/tRNA‑Ile
*NZ_CP009273349,9990TG41.7% 60.0 / 31.5 72V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NZ_CP009273497,0200AC41.4% 19.9 / 10.1 29*559E (TAA→GAA) ybaLKef family K(+) transporter
*NZ_CP0092734,086,4990CA41.3% 65.3 / 22.9 46V100V (GTG→GTTrhaML‑rhamnose mutarotase
*NZ_CP0092734,058,9580GA40.9% 47.8 / 22.4 44L57L (CTG→TTG) yihOMFS transporter
*NZ_CP009273595,0320TG40.0% 3.2 / 14.2 21V288G (GTG→GGG) cusACu(+)/Ag(+) efflux RND transporter permease subunit CusA
*NZ_CP0092734,570,4470AT40.0% 85.3 / 19.2 55L278Q (CTA→CAA) hsdStype I restriction‑modification system specificity subunit

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 433193062 (1.380)6 (0.130)
+CCA
3/146 3.2 8.8% coding (203/1356 nt) melA alpha‑galactosidase
?NZ_CP009273 4331931 = 64 (1.350)coding (204/1356 nt) melA alpha‑galactosidase
* ? NZ_CP009273 = 360653246 (0.970)3 (0.070) 3/142 3.2 7.2% coding (204/588 nt) acpT 4'‑phosphopantetheinyl transferase AcpT
?NZ_CP009273 = 3606543 36 (0.860)coding (215/588 nt) acpT 4'‑phosphopantetheinyl transferase AcpT
* ? NZ_CP009273 = 3575002141 (2.970)3 (0.070) 3/142 3.2 2.3% intergenic (+16/‑221) yhhY/hcp N‑acetyltransferase/Hcp1 family type VI secretion system effector
?NZ_CP009273 = 3575009 133 (3.160)intergenic (+23/‑214) yhhY/hcp N‑acetyltransferase/Hcp1 family type VI secretion system effector
* ? NZ_CP009273 = 349566567 (1.410)3 (0.070) 3/144 3.2 4.7% intergenic (+16/‑34) frlB/frlC fructoselysine 6‑phosphate deglycase/fructoselysine 3‑epimerase
?NZ_CP009273 = 3495669 62 (1.450)intergenic (+20/‑30) frlB/frlC fructoselysine 6‑phosphate deglycase/fructoselysine 3‑epimerase
* ? NZ_CP009273 = 288605151 (1.080)3 (0.070) 3/148 3.3 6.2% coding (36/1272 nt) ygcN FAD‑dependent oxidoreductase
?NZ_CP009273 = 2886060 43 (0.980)coding (45/1272 nt) ygcN FAD‑dependent oxidoreductase
* ? NZ_CP009273 = 45533964 (1.350)3 (0.070) 3/154 3.3 4.7% coding (996/2355 nt) lon endopeptidase La
?NZ_CP009273 = 455342 61 (1.340)coding (999/2355 nt) lon endopeptidase La