breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 60 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,154,0680TG72.7% ‑0.1 / 11.9 22G248G (GGT→GGGptsGPTS glucose transporter subunit IIBC
*NZ_CP0092732,141,2140TG70.6% ‑0.1 / 11.4 17G20G (GGT→GGGyegDmolecular chaperone
*NZ_CP0092733,806,4320AC64.7% 8.2 / 14.1 17T114T (ACA→ACCcoaBCbifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC
*NZ_CP009273349,9990TG61.9% 5.4 / 21.6 21V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NZ_CP0092731,125,8250TC61.1% 9.4 / 15.3 18G165G (GGA→GGGflgAflagellar basal body P‑ring formation chaperone FlgA
*NZ_CP0092734,558,3210TG58.8% 12.2 / 19.5 17T6P (ACC→CCC) mdtMmultidrug efflux MFS transporter MdtM
*NZ_CP0092732,380,3410GA57.9% ‑5.6 / 18.8 19intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092731,598,1800TG57.1% 8.8 / 19.7 23V302G (GTG→GGG) lsrCautoinducer 2 ABC transporter permease LsrC
*NZ_CP0092733,220,1080TG57.1% 8.4 / 19.2 21G172G (GGT→GGGygjIamino acid permease
*NZ_CP0092733,659,0620CA56.7% 11.2 / 39.2 30A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NZ_CP009273702,5970TG56.2% 9.9 / 15.0 16V350G (GTG→GGG) glnSglutamine‑‑tRNA ligase
*NZ_CP0092733,126,6610TG56.2% 3.8 / 18.9 16H219P (CAC→CCC) yghSdTMP kinase
*NZ_CP0092731,738,3120TG55.6% 12.2 / 16.0 18V200G (GTG→GGG) mdtKmultidrug efflux MATE transporter MdtK
*NZ_CP0092732,380,3570AT55.6% 8.3 / 23.6 18intergenic (+47/‑56)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP00927368,4430TG54.5% 6.1 / 10.5 11G202G (GGT→GGGyabIDedA family protein
*NZ_CP009273964,4020TG54.5% 8.9 / 11.9 11V194G (GTA→GGA) lpxKtetraacyldisaccharide 4'‑kinase
*NZ_CP0092731,853,9420AC54.5% 4.1 / 12.0 11V182G (GTA→GGA) ydjKMFS transporter
*NZ_CP009273760,1870GC53.8% 1.9 / 11.4 13P184P (CCG→CCCsucDsuccinate‑‑CoA ligase subunit alpha
*NZ_CP009273902,6740GA53.8% 0.4 / 13.4 13F355F (TTC→TTTybjTSDR family oxidoreductase
*NZ_CP0092733,753,7440AC53.8% 5.3 / 10.5 13V289G (GTG→GGG) selAL‑seryl‑tRNA(Sec) selenium transferase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 19212522 (0.810)4 (0.160) 4/154 1.8 16.4% coding (733/759 nt) ispU (2E,6E)‑farnesyl‑diphosphate‑specific ditrans,polycis‑undecaprenyl‑diphosphate synthase
?NZ_CP009273 = 192230 21 (0.850)coding (67/858 nt) cdsA phosphatidate cytidylyltransferase
* ? NZ_CP009273 = 443721532 (1.180)3 (0.130) 3/150 2.1 9.1% coding (3287/3780 nt) tamB autotransporter assembly complex protein TamB
?NZ_CP009273 = 4437296 32 (1.330)coding (3368/3780 nt) tamB autotransporter assembly complex protein TamB
* ? NZ_CP009273 4399442 =23 (0.850)3 (0.130) 3/150 2.1 12.4% coding (351/732 nt) rlmB 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
?NZ_CP009273 4399525 = 22 (0.920)coding (434/732 nt) rlmB 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NZ_CP009273 = 320953320 (0.740)3 (0.130) 3/150 2.1 14.1% coding (318/765 nt) nfeF NADPH‑dependent ferric chelate reductase
?NZ_CP009273 = 3209595 19 (0.790)coding (256/765 nt) nfeF NADPH‑dependent ferric chelate reductase
* ? NZ_CP009273 3122373 =38 (1.400)4 (0.170) 3/148 2.1 10.9% coding (743/765 nt) glcC transcriptional regulator GlcC
?NZ_CP009273 3122512 = 32 (1.350)coding (1027/1137 nt) yghO hypothetical protein
* ? NZ_CP009273 322260 =34 (1.250)3 (0.130) 3/150 2.1 10.0% coding (699/1671 nt) betA choline dehydrogenase
?NZ_CP009273 322363 = 24 (1.000)coding (596/1671 nt) betA choline dehydrogenase