breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 59 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP034943 | 3,285,647 | 0 | C | A | 42.1% | 298.0 / inf | 423 | V252L (GTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,580 | 0 | A | G | 41.7% | 345.9 / inf | 415 | I274T (ATA→ACA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,575 | 0 | T | G | 37.1% | 490.4 / inf | 404 | N276H (AAT→CAT) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 1,366,109 | 0 | G | T | 36.4% | 170.1 / 28.0 | 138 | D203Y (GAT→TAT) | EO946_RS07135 | PepSY domain‑containing protein |
* | NZ_CP034943 | 3,285,567 | 0 | C | T | 35.6% | 482.6 / inf | 405 | L278L (CTG→CTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 634,054 | 0 | C | G | 34.7% | 319.9 / 57.5 | 195 | E573Q (GAA→CAA) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
* | NZ_CP034943 | 1,730,096 | 0 | G | C | 32.6% | 131.0 / 25.5 | 103 | E173Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 2,981,253 | 0 | C | T | 31.6% | ‑3.8 / 13.1 | 19 | noncoding (1267/1550 nt) | EO946_RS15435 | 16S ribosomal RNA |
* | NZ_CP034943 | 1,366,115 | 0 | G | C | 31.0% | 241.3 / 24.3 | 142 | E205Q (GAA→CAA) | EO946_RS07135 | PepSY domain‑containing protein |
* | NZ_CP034943 | 3,285,713 | 0 | G | A | 29.4% | 307.6 / 155.7 | 177 | L230L (CTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 2,991,227 | 0 | T | G | 28.8% | 341.4 / 75.8 | 223 | intergenic (+28/‑179) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 2,991,236 | 0 | C | G | 27.8% | 386.3 / 52.4 | 208 | intergenic (+37/‑170) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 2,270,064 | 0 | G | C | 27.8% | 326.9 / 32.8 | 187 | L39V (CTA→GTA) | prsW | glutamic‑type intramembrane protease PrsW |
* | NZ_CP034943 | 1,730,093 | 0 | G | C | 27.3% | 160.8 / 12.1 | 102 | E172Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 1,730,099 | 0 | A | C | 27.2% | 159.5 / 11.6 | 103 | S174R (AGC→CGC) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 1,730,075 | 0 | G | C | 26.9% | 201.0 / 22.3 | 118 | E166Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 2,901,671 | 0 | A | C | 25.3% | 331.0 / 46.8 | 150 | K411T (AAA→ACA) | EO946_RS14945 | extracellular solute‑binding protein |
* | NZ_CP034943 | 886,751 | 0 | G | C | 25.1% | 414.1 / 21.4 | 196 | S46C (TCT→TGT) | EO946_RS04490 | MFS transporter |
* | NZ_CP034943 | 2,408,727 | 0 | C | A | 25.0% | 246.7 / 27.0 | 152 | A327S (GCG→TCG) | spoIIIAE | stage III sporulation protein AE |
* | NZ_CP034943 | 549,144 | 0 | A | C | 24.9% | 257.4 / 34.6 | 170 | Q52H (CAA→CAC) | EO946_RS02865 | PLP‑dependent aminotransferase family protein |
Marginal new junction evidence (lowest skew 10 of 21 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP034943 | 607695 = | 237 (0.860) | 37 (0.140) +AGTAGCCCCT |
26/248 | 3.8 | 11.6% | coding (538/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | = 607846 | 374 (1.350) | coding (689/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 607623 = | 273 (0.990) | 24 (0.090) +AGTAGCCCCT |
17/248 | 5.6 | 7.4% | coding (466/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | = 607846 | 374 (1.350) | coding (689/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 2557054 = | 258 (0.930) | 7 (0.030) | 7/242 | 8.7 | 2.9% | coding (180/756 nt) | EO946_RS13225 | metal‑binding protein ZinT |
? | NZ_CP034943 | = 2557050 | 234 (0.940) | coding (184/756 nt) | EO946_RS13225 | metal‑binding protein ZinT | |||||
* | ? | NZ_CP034943 | = 607646 | 257 (0.940) | 9 (0.030) +ACGGGAGCC |
7/246 | 8.8 | 3.6% | coding (489/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607683 = | 250 (0.900) | coding (526/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 607659 = | 239 (0.860) | 10 (0.040) +AGTAGCCCCT |
7/248 | 8.9 | 3.4% | coding (502/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | = 607846 | 374 (1.350) | coding (689/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607682 | 249 (0.910) | 8 (0.030) +ACGGGAGCC |
6/246 | 9.3 | 3.3% | coding (525/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607683 = | 249 (0.900) | coding (526/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 3592725 = | 300 (1.080) | 7 (0.030) | 5/248 | 9.9 | 2.5% | coding (424/702 nt) | urtE | urea ABC transporter ATP‑binding subunit UrtE |
? | NZ_CP034943 | = 3592721 | 267 (1.040) | coding (428/702 nt) | urtE | urea ABC transporter ATP‑binding subunit UrtE | |||||
* | ? | NZ_CP034943 | = 607256 | 370 (1.340) | 11 (0.040) | 5/262 | 10.2 | 3.3% | coding (99/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607428 = | 292 (1.080) | coding (271/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 2071634 | 216 (0.780) | 11 (0.040) +G |
5/266 | 10.3 | 4.8% | coding (2659/7377 nt) | EO946_RS10385 | NTTRR‑F1 domain |
? | NZ_CP034943 | 2071645 = | 226 (0.820) | coding (2648/7377 nt) | EO946_RS10385 | NTTRR‑F1 domain | |||||
* | ? | NZ_CP034943 | = 607149 | 482 (1.740) | 7 (0.030) +TGCCGGGAGTCATG |
4/240 | 10.3 | 1.7% | intergenic (+515/‑9) | EO946_RS03180/EO946_RS03185 | glycosyltransferase/collagen‑like protein |
? | NZ_CP034943 | 607794 = | 420 (1.520) | coding (637/1125 nt) | EO946_RS03185 | collagen‑like protein |