breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 59 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,6470CA42.1% 298.0 / inf 423V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5800AG41.7% 345.9 / inf 415I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5750TG37.1% 490.4 / inf 404N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP0349431,366,1090GT36.4% 170.1 / 28.0 138D203Y (GAT→TAT) EO946_RS07135PepSY domain‑containing protein
*NZ_CP0349433,285,5670CT35.6% 482.6 / inf 405L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP034943634,0540CG34.7% 319.9 / 57.5 195E573Q (GAA→CAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349431,730,0960GC32.6% 131.0 / 25.5 103E173Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349432,981,2530CT31.6% ‑3.8 / 13.1 19noncoding (1267/1550 nt)EO946_RS1543516S ribosomal RNA
*NZ_CP0349431,366,1150GC31.0% 241.3 / 24.3 142E205Q (GAA→CAA) EO946_RS07135PepSY domain‑containing protein
*NZ_CP0349433,285,7130GA29.4% 307.6 / 155.7 177L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349432,991,2270TG28.8% 341.4 / 75.8 223intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,991,2360CG27.8% 386.3 / 52.4 208intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,270,0640GC27.8% 326.9 / 32.8 187L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP0349431,730,0930GC27.3% 160.8 / 12.1 102E172Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349431,730,0990AC27.2% 159.5 / 11.6 103S174R (AGC→CGC) rodZcell shape determination protein RodZ
*NZ_CP0349431,730,0750GC26.9% 201.0 / 22.3 118E166Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349432,901,6710AC25.3% 331.0 / 46.8 150K411T (AAA→ACA) EO946_RS14945extracellular solute‑binding protein
*NZ_CP034943886,7510GC25.1% 414.1 / 21.4 196S46C (TCT→TGT) EO946_RS04490MFS transporter
*NZ_CP0349432,408,7270CA25.0% 246.7 / 27.0 152A327S (GCG→TCG) spoIIIAEstage III sporulation protein AE
*NZ_CP034943549,1440AC24.9% 257.4 / 34.6 170Q52H (CAA→CACEO946_RS02865PLP‑dependent aminotransferase family protein

Marginal new junction evidence (lowest skew 10 of 21 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 607695 =237 (0.860)37 (0.140)
+AGTAGCCCCT
26/248 3.8 11.6% coding (538/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 374 (1.350)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607623 =273 (0.990)24 (0.090)
+AGTAGCCCCT
17/248 5.6 7.4% coding (466/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 374 (1.350)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 2557054 =258 (0.930)7 (0.030) 7/242 8.7 2.9% coding (180/756 nt) EO946_RS13225 metal‑binding protein ZinT
?NZ_CP034943 = 2557050 234 (0.940)coding (184/756 nt) EO946_RS13225 metal‑binding protein ZinT
* ? NZ_CP034943 = 607646257 (0.940)9 (0.030)
+ACGGGAGCC
7/246 8.8 3.6% coding (489/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607683 = 250 (0.900)coding (526/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607659 =239 (0.860)10 (0.040)
+AGTAGCCCCT
7/248 8.9 3.4% coding (502/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 374 (1.350)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607682249 (0.910)8 (0.030)
+ACGGGAGCC
6/246 9.3 3.3% coding (525/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607683 = 249 (0.900)coding (526/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 3592725 =300 (1.080)7 (0.030) 5/248 9.9 2.5% coding (424/702 nt) urtE urea ABC transporter ATP‑binding subunit UrtE
?NZ_CP034943 = 3592721 267 (1.040)coding (428/702 nt) urtE urea ABC transporter ATP‑binding subunit UrtE
* ? NZ_CP034943 = 607256370 (1.340)11 (0.040) 5/262 10.2 3.3% coding (99/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607428 = 292 (1.080)coding (271/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 2071634216 (0.780)11 (0.040)
+G
5/266 10.3 4.8% coding (2659/7377 nt) EO946_RS10385 NTTRR‑F1 domain
?NZ_CP034943 2071645 = 226 (0.820)coding (2648/7377 nt) EO946_RS10385 NTTRR‑F1 domain
* ? NZ_CP034943 = 607149482 (1.740)7 (0.030)
+TGCCGGGAGTCATG
4/240 10.3 1.7% intergenic (+515/‑9) EO946_RS03180/EO946_RS03185 glycosyltransferase/collagen‑like protein
?NZ_CP034943 607794 = 420 (1.520)coding (637/1125 nt) EO946_RS03185 collagen‑like protein