breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 36 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP034943 | 3,285,647 | 0 | C | A | 39.9% | 248.1 / inf | 282 | V252L (GTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,580 | 0 | A | G | 37.8% | 287.4 / inf | 268 | I274T (ATA→ACA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 2,991,236 | 0 | C | G | 36.4% | 107.6 / 26.5 | 80 | intergenic (+37/‑170) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 3,285,575 | 0 | T | G | 34.5% | 340.1 / 271.3 | 275 | N276H (AAT→CAT) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 634,054 | 0 | C | G | 33.6% | 188.5 / 33.8 | 117 | E573Q (GAA→CAA) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
* | NZ_CP034943 | 3,285,567 | 0 | C | T | 32.3% | 392.2 / 280.5 | 279 | L278L (CTG→CTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 634,058 | 0 | C | G | 32.1% | 211.8 / 27.1 | 117 | K571N (AAG→AAC) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
* | NZ_CP034943 | 1,730,075 | 0 | G | C | 29.6% | 148.3 / 14.4 | 86 | E166Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 514,968 | 0 | G | T | 27.7% | 153.0 / 22.7 | 84 | V128L (GTA→TTA) | EO946_RS02645 | outer membrane lipoprotein carrier protein LolA |
* | NZ_CP034943 | 3,285,713 | 0 | G | A | 27.6% | 225.0 / 91.2 | 120 | L230L (CTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 2,991,233 | 0 | G | T | 27.4% | 127.7 / 13.3 | 84 | intergenic (+34/‑173) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 1,366,115 | 0 | G | C | 26.8% | 188.7 / 14.5 | 101 | E205Q (GAA→CAA) | EO946_RS07135 | PepSY domain‑containing protein |
* | NZ_CP034943 | 607,700 | 0 | A | C | 25.5% | 197.9 / 66.7 | 145 | E181D (GAA→GAC) | EO946_RS03185 | collagen‑like protein |
* | NZ_CP034943 | 2,830,523 | 0 | C | G | 25.4% | 265.3 / 20.8 | 128 | L89V (CTT→GTT) | brnQ | branched‑chain amino acid transport system II carrier protein |
* | NZ_CP034943 | 595,203 | 0 | C | A | 25.2% | 288.9 / 32.0 | 143 | T30K (ACG→AAG) | EO946_RS03130 | MMPL family transporter |
* | NZ_CP034943 | 2,991,252 | 0 | C | A | 25.0% | 142.0 / 10.3 | 75 | intergenic (+53/‑154) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 2,991,227 | 0 | T | G | 24.7% | 142.7 / 14.3 | 90 | intergenic (+28/‑179) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 17,887 | 0 | G | T | 24.1% | 226.7 / 15.4 | 117 | E248D (GAG→GAT) | dacA | D‑alanyl‑D‑alanine carboxypeptidase |
* | NZ_CP034943 | 240,198 | 0 | G | T | 24.1% | 314.2 / 30.8 | 170 | Q112K (CAA→AAA) | EO946_RS01360 | hypothetical protein |
* | NZ_CP034943 | 2,991,245 | 0 | G | T | 24.1% | 129.2 / 19.9 | 83 | intergenic (+46/‑161) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
Marginal new junction evidence (lowest skew 10 of 11 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP034943 | = 607279 | 218 (1.330) | 13 (0.190) +79 bp |
6/112 | 3.5 | 13.9% | coding (122/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607323 = | 176 (1.080) | coding (166/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607149 | 228 (1.390) | 7 (0.050) +TGCCGGGAGTCATG |
5/242 | 7.6 | 3.1% | intergenic (+515/‑9) | EO946_RS03180/EO946_RS03185 | glycosyltransferase/collagen‑like protein |
? | NZ_CP034943 | 607794 = | 262 (1.600) | coding (637/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 2071641 = | 101 (0.620) | 31 (0.190) | 5/264 | 8.1 | 23.9% | coding (2652/7377 nt) | EO946_RS10385 | NTTRR‑F1 domain |
? | NZ_CP034943 | = 2072009 | NA (NA) | coding (2284/7377 nt) | EO946_RS10385 | NTTRR‑F1 domain | |||||
* | ? | NZ_CP034943 | = 607429 | 142 (0.870) | 5 (0.030) | 4/238 | 8.1 | 3.5% | coding (272/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607727 = | 149 (1.040) | coding (570/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 982440 = | 156 (0.950) | 4 (0.030) | 4/248 | 8.3 | 2.7% | coding (851/1443 nt) | EO946_RS05080 | peptidoglycan endopeptidase |
? | NZ_CP034943 | = 982436 | 143 (0.950) | coding (855/1443 nt) | EO946_RS05080 | peptidoglycan endopeptidase | |||||
* | ? | NZ_CP034943 | = 607355 | 183 (1.120) | 9 (0.060) | 4/260 | 8.5 | 4.4% | coding (198/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607752 = | 218 (1.390) | coding (595/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607409 | 152 (0.930) | 10 (0.060) | 3/264 | 9.2 | 6.0% | coding (252/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607527 = | 166 (1.040) | coding (370/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607409 | 152 (0.930) | 10 (0.060) | 3/264 | 9.2 | 6.0% | coding (252/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607482 = | 167 (1.040) | coding (325/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607256 | 225 (1.380) | 3 (0.020) | 3/264 | 9.2 | 1.5% | coding (99/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607527 = | 166 (1.040) | coding (370/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607256 | 225 (1.380) | 3 (0.020) | 3/264 | 9.2 | 1.5% | coding (99/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607482 = | 167 (1.040) | coding (325/1125 nt) | EO946_RS03185 | collagen‑like protein |