breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 36 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,6470CA39.9% 248.1 / inf 282V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5800AG37.8% 287.4 / inf 268I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349432,991,2360CG36.4% 107.6 / 26.5 80intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349433,285,5750TG34.5% 340.1 / 271.3 275N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP034943634,0540CG33.6% 188.5 / 33.8 117E573Q (GAA→CAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349433,285,5670CT32.3% 392.2 / 280.5 279L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP034943634,0580CG32.1% 211.8 / 27.1 117K571N (AAG→AACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349431,730,0750GC29.6% 148.3 / 14.4 86E166Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP034943514,9680GT27.7% 153.0 / 22.7 84V128L (GTA→TTA) EO946_RS02645outer membrane lipoprotein carrier protein LolA
*NZ_CP0349433,285,7130GA27.6% 225.0 / 91.2 120L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349432,991,2330GT27.4% 127.7 / 13.3 84intergenic (+34/‑173)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349431,366,1150GC26.8% 188.7 / 14.5 101E205Q (GAA→CAA) EO946_RS07135PepSY domain‑containing protein
*NZ_CP034943607,7000AC25.5% 197.9 / 66.7 145E181D (GAA→GACEO946_RS03185collagen‑like protein
*NZ_CP0349432,830,5230CG25.4% 265.3 / 20.8 128L89V (CTT→GTT) brnQbranched‑chain amino acid transport system II carrier protein
*NZ_CP034943595,2030CA25.2% 288.9 / 32.0 143T30K (ACG→AAG) EO946_RS03130MMPL family transporter
*NZ_CP0349432,991,2520CA25.0% 142.0 / 10.3 75intergenic (+53/‑154)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,991,2270TG24.7% 142.7 / 14.3 90intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP03494317,8870GT24.1% 226.7 / 15.4 117E248D (GAG→GATdacAD‑alanyl‑D‑alanine carboxypeptidase
*NZ_CP034943240,1980GT24.1% 314.2 / 30.8 170Q112K (CAA→AAA) EO946_RS01360hypothetical protein
*NZ_CP0349432,991,2450GT24.1% 129.2 / 19.9 83intergenic (+46/‑161)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA

Marginal new junction evidence (lowest skew 10 of 11 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 607279218 (1.330)13 (0.190)
+79 bp
6/112 3.5 13.9% coding (122/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607323 = 176 (1.080)coding (166/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607149228 (1.390)7 (0.050)
+TGCCGGGAGTCATG
5/242 7.6 3.1% intergenic (+515/‑9) EO946_RS03180/EO946_RS03185 glycosyltransferase/collagen‑like protein
?NZ_CP034943 607794 = 262 (1.600)coding (637/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 2071641 =101 (0.620)31 (0.190) 5/264 8.1 23.9% coding (2652/7377 nt) EO946_RS10385 NTTRR‑F1 domain
?NZ_CP034943 = 2072009 NA (NA)coding (2284/7377 nt) EO946_RS10385 NTTRR‑F1 domain
* ? NZ_CP034943 = 607429142 (0.870)5 (0.030) 4/238 8.1 3.5% coding (272/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607727 = 149 (1.040)coding (570/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 982440 =156 (0.950)4 (0.030) 4/248 8.3 2.7% coding (851/1443 nt) EO946_RS05080 peptidoglycan endopeptidase
?NZ_CP034943 = 982436 143 (0.950)coding (855/1443 nt) EO946_RS05080 peptidoglycan endopeptidase
* ? NZ_CP034943 = 607355183 (1.120)9 (0.060) 4/260 8.5 4.4% coding (198/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607752 = 218 (1.390)coding (595/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607409152 (0.930)10 (0.060) 3/264 9.2 6.0% coding (252/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607527 = 166 (1.040)coding (370/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607409152 (0.930)10 (0.060) 3/264 9.2 6.0% coding (252/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607482 = 167 (1.040)coding (325/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607256225 (1.380)3 (0.020) 3/264 9.2 1.5% coding (99/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607527 = 166 (1.040)coding (370/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607256225 (1.380)3 (0.020) 3/264 9.2 1.5% coding (99/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607482 = 167 (1.040)coding (325/1125 nt) EO946_RS03185 collagen‑like protein