breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 31 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 341,377 | 0 | T | G | 41.6% | 168.4 / 44.2 | 137 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 1,091,992 | 0 | T | G | 40.8% | 455.4 / 98.8 | 294 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 1,421,147 | 0 | T | G | 34.6% | 395.0 / 24.6 | 238 | T79P (ACC→CCC) | ydhB | predicted DNA‑binding transcriptional regulator |
* | AP012306 | 644,417 | 0 | G | A | 30.2% | 832.3 / inf | 536 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 3,527,368 | 0 | A | C | 29.6% | 639.7 / 91.9 | 294 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 800,675 | 0 | A | C | 29.6% | 567.0 / 45.6 | 257 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 1,226,557 | 0 | A | G | 29.4% | 358.0 / 86.2 | 180 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 1,944,288 | 0 | A | C | 28.8% | 529.4 / 69.3 | 254 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 3,938,887 | 0 | C | T | 28.7% | 476.7 / 150.0 | 244 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 3,372,701 | 0 | T | G | 27.7% | 121.2 / 25.6 | 65 | noncoding (54/76 nt) | gltU | tRNA‑Glu |
* | AP012306 | 3,020,670 | 0 | T | G | 26.0% | 385.8 / 15.2 | 196 | K195N (AAA→AAC) | glgC | glucose‑1‑phosphate adenylyltransferase |
* | AP012306 | 3,639,040 | 0 | T | G | 25.6% | 179.4 / 15.2 | 82 | noncoding (54/76 nt) | gltV | tRNA‑Glu |
* | AP012306 | 2,212,753 | 0 | A | C | 25.5% | 362.4 / 38.3 | 145 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 1,854,851 | 0 | A | C | 25.2% | 780.9 / 57.6 | 311 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 2,758,909 | 0 | T | G | 24.7% | 583.6 / 14.8 | 219 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 1,128,412 | 0 | T | G | 24.0% | 754.6 / 50.2 | 300 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 935,054 | 0 | G | T | 23.8% | 303.7 / 44.3 | 105 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 3,903,791 | 0 | A | G | 22.6% | 638.7 / 81.4 | 261 | E189G (GAA→GGA) | yjgJ | predicted transcriptional regulator |
* | AP012306 | 3,590,367 | 0 | A | C | 22.6% | 827.9 / 37.1 | 301 | T30P (ACC→CCC) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 2,376,612 | 0 | T | G | 22.6% | 470.5 / 27.8 | 186 | V326G (GTA→GGA) | ygbN | predicted transporter |
Marginal new junction evidence (lowest skew 10 of 94 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 997497 | 333 (0.980) | 19 (0.060) | 18/268 | 13.3 | 6.2% | coding (41/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
? | AP012306 | 997993 = | 279 (0.910) | coding (537/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase | |||||
* | ? | AP012306 | = 1092512 | 428 (1.260) | 12 (0.040) | 9/258 | 16.2 | 3.5% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 298 (1.020) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | 644310 = | 333 (0.980) | 28 (0.080) +GAG |
11/292 | 16.2 | 7.7% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073420 | 354 (1.040) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | 644310 = | 333 (0.980) | 26 (0.080) +G |
9/296 | 17.1 | 7.5% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073542 | 314 (0.930) | intergenic (+2/‑45) | valX/valY | tRNA‑Val/tRNA‑Val | |||||
* | ? | AP012306 | 228777 = | 768 (2.260) | 7 (0.020) | 7/278 | 17.6 | 0.95% | coding (275/771 nt) | yafV | predicted C‑N hydrolase family amidase, NAD(P)‑binding |
? | AP012306 | 228787 = | 737 (2.330) | coding (265/771 nt) | yafV | predicted C‑N hydrolase family amidase, NAD(P)‑binding | |||||
* | ? | AP012306 | = 215364 | NA (NA) | 7 (0.020) | 7/288 | 17.8 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | 3353019 = | 423 (1.250) | 7 (0.020) | 7/296 | 18.0 | 1.7% | coding (1828/1890 nt) | gidA | glucose‑inhibited cell‑division protein |
? | AP012306 | 3353034 = | 410 (1.220) | coding (1813/1890 nt) | gidA | glucose‑inhibited cell‑division protein | |||||
* | ? | AP012306 | = 1215165 | 440 (1.300) | 6 (0.020) | 6/286 | 18.3 | 1.4% | coding (147/669 nt) | ydcL | predicted lipoprotein |
? | AP012306 | = 1215167 | 424 (1.300) | coding (149/669 nt) | ydcL | predicted lipoprotein | |||||
* | ? | AP012306 | = 617362 | 363 (1.070) | 6 (0.020) | 6/288 | 18.4 | 1.7% | intergenic (‑161/‑548) | gltA/sdhC | citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
? | AP012306 | = 617373 | 349 (1.060) | intergenic (‑172/‑537) | gltA/sdhC | citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556 | |||||
* | ? | AP012306 | = 1178769 | 316 (0.930) | 5 (0.020) | 5/274 | 18.6 | 1.7% | coding (451/843 nt) | ycjP | predicted sugar transporter subunit |
? | AP012306 | = 1178757 | 291 (0.930) | coding (439/843 nt) | ycjP | predicted sugar transporter subunit |