breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 31 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG41.6% 168.4 / 44.2 137G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123061,091,9920TG40.8% 455.4 / 98.8 294intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,421,1470TG34.6% 395.0 / 24.6 238T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP012306644,4170GA30.2% 832.3 / inf 536intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC29.6% 639.7 / 91.9 294L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306800,6750AC29.6% 567.0 / 45.6 257T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123061,226,5570AG29.4% 358.0 / 86.2 180G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,944,2880AC28.8% 529.4 / 69.3 254G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,938,8870CT28.7% 476.7 / 150.0 244S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,372,7010TG27.7% 121.2 / 25.6 65noncoding (54/76 nt)gltUtRNA‑Glu
*AP0123063,020,6700TG26.0% 385.8 / 15.2 196K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP0123063,639,0400TG25.6% 179.4 / 15.2 82noncoding (54/76 nt)gltVtRNA‑Glu
*AP0123062,212,7530AC25.5% 362.4 / 38.3 145Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123061,854,8510AC25.2% 780.9 / 57.6 311T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123062,758,9090TG24.7% 583.6 / 14.8 219G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123061,128,4120TG24.0% 754.6 / 50.2 300V29G (GTG→GGG) yciOconserved protein
*AP012306935,0540GT23.8% 303.7 / 44.3 105intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123063,903,7910AG22.6% 638.7 / 81.4 261E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123063,590,3670AC22.6% 827.9 / 37.1 301T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123062,376,6120TG22.6% 470.5 / 27.8 186V326G (GTA→GGA) ygbNpredicted transporter

Marginal new junction evidence (lowest skew 10 of 94 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 997497333 (0.980)19 (0.060) 18/268 13.3 6.2% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 279 (0.910)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092512428 (1.260)12 (0.040) 9/258 16.2 3.5% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 298 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 644310 =333 (0.980)28 (0.080)
+GAG
11/292 16.2 7.7% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 354 (1.040)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 644310 =333 (0.980)26 (0.080)
+G
9/296 17.1 7.5% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073542 314 (0.930)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val
* ? AP012306 228777 =768 (2.260)7 (0.020) 7/278 17.6 0.95% coding (275/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
?AP012306 228787 = 737 (2.330)coding (265/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
* ? AP012306 = 215364NA (NA)7 (0.020) 7/288 17.8 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 3353019 =423 (1.250)7 (0.020) 7/296 18.0 1.7% coding (1828/1890 nt) gidA glucose‑inhibited cell‑division protein
?AP012306 3353034 = 410 (1.220)coding (1813/1890 nt) gidA glucose‑inhibited cell‑division protein
* ? AP012306 = 1215165440 (1.300)6 (0.020) 6/286 18.3 1.4% coding (147/669 nt) ydcL predicted lipoprotein
?AP012306 = 1215167 424 (1.300)coding (149/669 nt) ydcL predicted lipoprotein
* ? AP012306 = 617362363 (1.070)6 (0.020) 6/288 18.4 1.7% intergenic (‑161/‑548) gltA/sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
?AP012306 = 617373 349 (1.060)intergenic (‑172/‑537) gltA/sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
* ? AP012306 = 1178769316 (0.930)5 (0.020) 5/274 18.6 1.7% coding (451/843 nt) ycjP predicted sugar transporter subunit
?AP012306 = 1178757 291 (0.930)coding (439/843 nt) ycjP predicted sugar transporter subunit