breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 241 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 1,747,903 | 0 | G | T | 57.2% | 61.5 / inf | 463 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 2,103,887 | 2 | . | A | 50.0% | ‑2.5 / 35.6 | 14 | coding (154/216 nt) | ECB_01992 | hypothetical protein |
* | REL606 | 2,103,887 | 3 | . | G | 50.0% | 0.1 / 35.1 | 14 | coding (154/216 nt) | ECB_01992 | hypothetical protein |
* | REL606 | 2,103,887 | 4 | . | C | 50.0% | ‑1.5 / 39.6 | 14 | coding (154/216 nt) | ECB_01992 | hypothetical protein |
* | REL606 | 2,515,909 | 0 | A | C | 48.5% | 90.1 / 24.4 | 68 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 4,111,429 | 0 | T | G | 48.5% | 86.7 / 16.2 | 68 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 2,103,887 | 1 | . | C | 46.2% | 1.4 / 33.4 | 14 | coding (154/216 nt) | ECB_01992 | hypothetical protein |
* | REL606 | 3,472,700 | 0 | C | G | 43.9% | 32.6 / 73.2 | 66 | A290G (GCG→GGG) | yhgA | predicted transposase |
* | REL606 | 4,180,249 | 0 | T | G | 43.8% | 134.5 / 39.1 | 98 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 1,853,067 | 0 | A | C | 42.1% | 159.9 / 144.2 | 160 | T56P (ACC→CCC) | yeaN | predicted transporter |
* | REL606 | 433 | 0 | T | G | 40.5% | 129.1 / 32.6 | 84 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 1,854,401 | 0 | A | C | 40.5% | 130.7 / 36.6 | 89 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 3,229,011 | 0 | A | C | 39.6% | 122.0 / 77.7 | 101 | T126T (ACA→ACC) | yraM | hypothetical protein |
* | REL606 | 2,437,406 | 0 | G | T | 38.6% | 65.5 / 27.0 | 44 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 955,035 | 0 | A | C | 37.9% | 294.7 / 25.8 | 145 | T192P (ACC→CCC) | lolA | outer‑membrane lipoprotein carrier protein precursor |
* | REL606 | 4,392,556 | 0 | C | T | 37.1% | 171.8 / 80.4 | 140 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 931,314 | 0 | T | C | 37.0% | 84.0 / 28.4 | 81 | E84G (GAG→GGG) | ybjE | predicted transporter |
* | REL606 | 895,856 | 0 | G | C | 36.0% | 34.7 / 19.5 | 25 | A26P (GCC→CCC) | ECB_00836 | putative Regulatory protein |
* | REL606 | 3,217,434 | 0 | G | C | 35.6% | 48.1 / 33.0 | 59 | A76P (GCC→CCC) | agaS | tagatose‑6‑phosphate ketose/aldose isomerase |
* | REL606 | 159,654 | 0 | A | C | 35.3% | 517.7 / 44.4 | 258 | G27G (GGT→GGG) | yadN | predicted fimbrial‑like adhesin protein |
Marginal new junction evidence (lowest skew 10 of 19 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 2103887 | 4 (0.030) | 81 (0.360) +CAGC |
24/80 | 4.8 | 89.6% | coding (154/216 nt) | ECB_01992 | hypothetical protein |
? | REL606 | 2103888 = | 13 (0.050) | coding (155/216 nt) | ECB_01992 | hypothetical protein | |||||
* | ? | REL606 | 2103888 = | 75 (0.310) | 39 (0.260) | 25/96 | 6.0 | 58.4% | coding (155/216 nt) | ECB_01992 | hypothetical protein |
? | REL606 | = 2103891 | 8 (0.050) | coding (158/216 nt) | ECB_01992 | hypothetical protein | |||||
* | ? | REL606 | 2015709 = | 188 (0.780) | 8 (0.040) | 6/134 | 16.3 | 3.8% | coding (260/1059 nt) | yeeE | predicted inner membrane protein |
? | REL606 | 4217041 = | 242 (1.130) | coding (476/2097 nt) | yjbH | predicted porin | |||||
* | ? | REL606 | 2066317 = | 274 (1.140) | 4 (0.020) | 4/128 | 17.3 | 1.8% | coding (287/840 nt) | wcaA | predicted glycosyl transferase |
? | REL606 | = 2066313 | 213 (1.040) | coding (291/840 nt) | wcaA | predicted glycosyl transferase | |||||
* | ? | REL606 | = 3320268 | 328 (1.360) | 16 (0.070) | 5/146 | 17.7 | 4.3% | intergenic (‑349/+81) | tldD/yhdP | predicted peptidase/conserved membrane protein, predicted transporter |
? | REL606 | = 4603900 | 398 (1.700) | intergenic (+406/+14) | yjjV/yjjW | predicted DNase/predicted pyruvate formate lyase activating enzyme | |||||
* | ? | REL606 | = 3320177 | 158 (0.660) | 13 (0.060) | 5/146 | 17.7 | 4.5% | intergenic (‑258/+172) | tldD/yhdP | predicted peptidase/conserved membrane protein, predicted transporter |
? | REL606 | = 4603900 | 398 (1.700) | intergenic (+406/+14) | yjjV/yjjW | predicted DNase/predicted pyruvate formate lyase activating enzyme | |||||
* | ? | REL606 | 3627801 = | 259 (1.080) | 4 (0.020) | 4/136 | 17.8 | 1.7% | coding (96/1680 nt) | yhjU | predicted inner membrane protein |
? | REL606 | = 3741644 | NA (NA) | noncoding (322/768 nt) | IS1 | repeat region | |||||
* | ? | REL606 | = 1609962 | 213 (0.890) | 3 (0.010) | 3/128 | 18.1 | 1.8% | coding (12/174 nt) | gnsB | predicted protein |
? | REL606 | = 1610346 | 143 (0.700) | intergenic (‑45/+103) | ynfN/ECB_01516 | predicted protein/putative cold‑shock protein | |||||
* | ? | REL606 | 1425651 = | 57 (0.240) | 5 (0.020) | 3/132 | 18.3 | 3.4% | coding (213/267 nt) | ynaE | predicted DNA‑binding transcriptional regulator |
? | REL606 | = 1600638 | 234 (1.110) | intergenic (‑586/‑167) | ydfJ/ydfK | predicted transporter/predicted DNA‑binding transcriptional regulator | |||||
* | ? | REL606 | 3026196 = | 250 (1.040) | 3 (0.010) | 3/134 | 18.4 | 1.5% | coding (607/1377 nt) | ECB_02826 | hypothetical protein |
? | REL606 | 3026684 = | 160 (0.750) | coding (119/1377 nt) | ECB_02826 | hypothetical protein |