breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 241 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,747,9030GT57.2% 61.5 / inf 463A8S (GCA→TCA) ydiLhypothetical protein
*REL6062,103,8872.A50.0% ‑2.5 / 35.6 14coding (154/216 nt)ECB_01992hypothetical protein
*REL6062,103,8873.G50.0% 0.1 / 35.1 14coding (154/216 nt)ECB_01992hypothetical protein
*REL6062,103,8874.C50.0% ‑1.5 / 39.6 14coding (154/216 nt)ECB_01992hypothetical protein
*REL6062,515,9090AC48.5% 90.1 / 24.4 68V260G (GTG→GGG) ypfIpredicted hydrolase
*REL6064,111,4290TG48.5% 86.7 / 16.2 68G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL6062,103,8871.C46.2% 1.4 / 33.4 14coding (154/216 nt)ECB_01992hypothetical protein
*REL6063,472,7000CG43.9% 32.6 / 73.2 66A290G (GCG→GGG) yhgApredicted transposase
*REL6064,180,2490TG43.8% 134.5 / 39.1 98G24G (GGT→GGGyjaHhypothetical protein
*REL6061,853,0670AC42.1% 159.9 / 144.2 160T56P (ACC→CCC) yeaNpredicted transporter
*REL6064330TG40.5% 129.1 / 32.6 84V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6061,854,4010AC40.5% 130.7 / 36.6 89K88N (AAA→AACyeaOhypothetical protein
*REL6063,229,0110AC39.6% 122.0 / 77.7 101T126T (ACA→ACCyraMhypothetical protein
*REL6062,437,4060GT38.6% 65.5 / 27.0 44D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL606955,0350AC37.9% 294.7 / 25.8 145T192P (ACC→CCC) lolAouter‑membrane lipoprotein carrier protein precursor
*REL6064,392,5560CT37.1% 171.8 / 80.4 140L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL606931,3140TC37.0% 84.0 / 28.4 81E84G (GAG→GGG) ybjEpredicted transporter
*REL606895,8560GC36.0% 34.7 / 19.5 25A26P (GCC→CCC) ECB_00836putative Regulatory protein
*REL6063,217,4340GC35.6% 48.1 / 33.0 59A76P (GCC→CCC) agaStagatose‑6‑phosphate ketose/aldose isomerase
*REL606159,6540AC35.3% 517.7 / 44.4 258G27G (GGT→GGGyadNpredicted fimbrial‑like adhesin protein

Marginal new junction evidence (lowest skew 10 of 19 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 21038874 (0.030)81 (0.360)
+CAGC
24/80 4.8 89.6% coding (154/216 nt) ECB_01992 hypothetical protein
?REL606 2103888 = 13 (0.050)coding (155/216 nt) ECB_01992 hypothetical protein
* ? REL606 2103888 =75 (0.310)39 (0.260) 25/96 6.0 58.4% coding (155/216 nt) ECB_01992 hypothetical protein
?REL606 = 2103891 8 (0.050)coding (158/216 nt) ECB_01992 hypothetical protein
* ? REL606 2015709 =188 (0.780)8 (0.040) 6/134 16.3 3.8% coding (260/1059 nt) yeeE predicted inner membrane protein
?REL606 4217041 = 242 (1.130)coding (476/2097 nt) yjbH predicted porin
* ? REL606 2066317 =274 (1.140)4 (0.020) 4/128 17.3 1.8% coding (287/840 nt) wcaA predicted glycosyl transferase
?REL606 = 2066313 213 (1.040)coding (291/840 nt) wcaA predicted glycosyl transferase
* ? REL606 = 3320268328 (1.360)16 (0.070) 5/146 17.7 4.3% intergenic (‑349/+81) tldD/yhdP predicted peptidase/conserved membrane protein, predicted transporter
?REL606 = 4603900 398 (1.700)intergenic (+406/+14) yjjV/yjjW predicted DNase/predicted pyruvate formate lyase activating enzyme
* ? REL606 = 3320177158 (0.660)13 (0.060) 5/146 17.7 4.5% intergenic (‑258/+172) tldD/yhdP predicted peptidase/conserved membrane protein, predicted transporter
?REL606 = 4603900 398 (1.700)intergenic (+406/+14) yjjV/yjjW predicted DNase/predicted pyruvate formate lyase activating enzyme
* ? REL606 3627801 =259 (1.080)4 (0.020) 4/136 17.8 1.7% coding (96/1680 nt) yhjU predicted inner membrane protein
?REL606 = 3741644 NA (NA)noncoding (322/768 nt) IS1 repeat region
* ? REL606 = 1609962213 (0.890)3 (0.010) 3/128 18.1 1.8% coding (12/174 nt) gnsB predicted protein
?REL606 = 1610346 143 (0.700)intergenic (‑45/+103) ynfN/ECB_01516 predicted protein/putative cold‑shock protein
* ? REL606 1425651 =57 (0.240)5 (0.020) 3/132 18.3 3.4% coding (213/267 nt) ynaE predicted DNA‑binding transcriptional regulator
?REL606 = 1600638 234 (1.110)intergenic (‑586/‑167) ydfJ/ydfK predicted transporter/predicted DNA‑binding transcriptional regulator
* ? REL606 3026196 =250 (1.040)3 (0.010) 3/134 18.4 1.5% coding (607/1377 nt) ECB_02826 hypothetical protein
?REL606 3026684 = 160 (0.750)coding (119/1377 nt) ECB_02826 hypothetical protein