breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 3,492,082 | 0 | T | G | 45.6% | 70.0 / 44.5 | 68 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 1,004,106 | 0 | A | C | 36.4% | 125.9 / 21.5 | 66 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
* | REL606 | 1,875,483 | 0 | G | C | 35.2% | 228.6 / 149.6 | 142 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 273,581 | 0 | T | G | 35.1% | 145.4 / 13.2 | 74 | G136G (GGT→GGG) | yagU | conserved inner membrane protein |
* | REL606 | 4,393,061 | 0 | T | G | 34.0% | 81.6 / 21.2 | 47 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 1,747,903 | 0 | G | T | 32.1% | 221.7 / 96.4 | 106 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 3,983,700 | 0 | T | G | 31.6% | 71.6 / 10.7 | 39 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 275,418 | 0 | A | C | 31.1% | 91.3 / 10.8 | 45 | F528V (TTT→GTT) | yagW | predicted receptor |
* | REL606 | 2,877,077 | 0 | A | C | 30.4% | 205.6 / 14.7 | 92 | intergenic (‑274/+41) | araE/kduD | arabinose transporter/2‑deoxy‑D‑gluconate 3‑dehydrogenase |
* | REL606 | 2,448,098 | 0 | T | G | 29.7% | 135.3 / 18.3 | 64 | F278V (TTT→GTT) | nupC | nucleoside (except guanosine) transporter |
* | REL606 | 2,631,772 | 0 | A | C | 27.3% | 142.7 / 27.3 | 66 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 4,141,009 | 0 | G | A | 27.1% | 117.9 / 16.8 | 48 | E28K (GAA→AAA) | yijC | DNA‑binding transcriptional repressor |
* | REL606 | 1,176,010 | 0 | T | G | 26.6% | 179.0 / 20.0 | 79 | A37A (GCA→GCC) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
* | REL606 | 4,169,232 | 0 | A | T | 26.4% | 177.3 / 22.7 | 72 | intergenic (+48/‑165) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 2,296,329 | 0 | A | C | 25.3% | 178.4 / 26.5 | 75 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 4,322,565 | 0 | T | G | 23.3% | 148.4 / 10.6 | 60 | G157G (GGT→GGG) | melB | melibiose:sodium symporter |
* | REL606 | 1,882,526 | 0 | T | G | 22.8% | 175.7 / 10.9 | 79 | I21S (ATT→AGT) | manZ | mannose‑specific enzyme IID component of PTS |
* | REL606 | 4,574,252 | 0 | T | G | 21.3% | 118.3 / 12.9 | 47 | S5S (TCA→TCC) | hpaX | hypothetical 4‑hydroxyphenylacetate permease |
Marginal new junction evidence (lowest skew 10 of 17 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 3180864 = | 91 (1.300) | 6 (0.090) | 6/136 | 6.5 | 6.5% | coding (58/663 nt) | yqjA | conserved inner membrane protein |
? | REL606 | 3180885 = | 89 (1.400) | coding (79/663 nt) | yqjA | conserved inner membrane protein | |||||
* | ? | REL606 | 1643503 = | 59 (0.840) | 4 (0.060) +CATCGATGG |
4/132 | 7.4 | 7.2% | coding (994/1257 nt) | ynfJ | putative voltage‑gated ClC‑type chloride channel ClcB |
? | REL606 | 1643503 = | 59 (0.840) | coding (994/1257 nt) | ynfJ | putative voltage‑gated ClC‑type chloride channel ClcB | |||||
* | ? | REL606 | 1643503 = | 59 (0.840) | 4 (0.060) | 4/138 | 7.6 | 5.3% | coding (994/1257 nt) | ynfJ | putative voltage‑gated ClC‑type chloride channel ClcB |
? | REL606 | 3180870 = | 89 (1.380) | coding (64/663 nt) | yqjA | conserved inner membrane protein | |||||
* | ? | REL606 | = 2791817 | 99 (1.410) | 7 (0.110) | 4/142 | 7.7 | 7.1% | coding (1422/1479 nt) | ygcE | predicted kinase |
? | REL606 | = 2792302 | 89 (1.340) | intergenic (+428/‑207) | ygcE/ECB_02621 | predicted kinase/conserved hypothetical protein | |||||
* | ? | REL606 | 2039188 = | 95 (1.360) | 9 (0.140) | 4/140 | 7.7 | 8.0% | coding (507/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | 2039561 = | 119 (1.820) | coding (134/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | 2036748 = | 93 (1.330) | 4 (0.060) | 4/140 | 7.7 | 4.0% | coding (211/1113 nt) | wbbC | putative glycosyltransferase WbbC |
? | REL606 | 2036931 = | 105 (1.610) | coding (28/1113 nt) | wbbC | putative glycosyltransferase WbbC | |||||
* | ? | REL606 | 1325040 = | 47 (0.670) | 4 (0.070) +TGTTATAACA |
3/130 | 7.9 | 9.0% | intergenic (+29/‑184) | yciQ/yciL | hypothetical protein; b1268_1/23S rRNA pseudouridylate synthase |
? | REL606 | 1325040 = | 47 (0.670) | intergenic (+29/‑184) | yciQ/yciL | hypothetical protein; b1268_1/23S rRNA pseudouridylate synthase | |||||
* | ? | REL606 | 3564938 = | 121 (1.730) | 3 (0.050) | 3/136 | 8.1 | 2.6% | coding (210/1608 nt) | yhiKL | hypothetical protein |
? | REL606 | 3565315 = | 111 (1.750) | intergenic (‑168/‑215) | yhiKL/yhiM | hypothetical protein/conserved inner membrane protein | |||||
* | ? | REL606 | 3737289 = | 92 (1.310) | 3 (0.050) | 3/138 | 8.2 | 3.2% | coding (538/1254 nt) | waaL | Lipid A‑core, surface polymer ligase |
? | REL606 | 3737758 = | 97 (1.510) | coding (69/1254 nt) | waaL | Lipid A‑core, surface polymer ligase | |||||
* | ? | REL606 | 3026311 = | 118 (1.680) | 3 (0.050) | 3/140 | 8.3 | 3.0% | coding (492/1377 nt) | ECB_02826 | hypothetical protein |
? | REL606 | 3026805 = | 87 (1.330) | intergenic (‑3/+206) | ECB_02826/ECB_02827 | hypothetical protein/Polysialic acid transport ATP‑binding KpsT |