breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,492,0820TG45.6% 70.0 / 44.5 68V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6061,004,1060AC36.4% 125.9 / 21.5 66intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL6061,875,4830GC35.2% 228.6 / 149.6 142intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL606273,5810TG35.1% 145.4 / 13.2 74G136G (GGT→GGGyagUconserved inner membrane protein
*REL6064,393,0610TG34.0% 81.6 / 21.2 47V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6061,747,9030GT32.1% 221.7 / 96.4 106A8S (GCA→TCA) ydiLhypothetical protein
*REL6063,983,7000TG31.6% 71.6 / 10.7 39T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL606275,4180AC31.1% 91.3 / 10.8 45F528V (TTT→GTT) yagWpredicted receptor
*REL6062,877,0770AC30.4% 205.6 / 14.7 92intergenic (‑274/+41)araE/kduDarabinose transporter/2‑deoxy‑D‑gluconate 3‑dehydrogenase
*REL6062,448,0980TG29.7% 135.3 / 18.3 64F278V (TTT→GTT) nupCnucleoside (except guanosine) transporter
*REL6062,631,7720AC27.3% 142.7 / 27.3 66intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL6064,141,0090GA27.1% 117.9 / 16.8 48E28K (GAA→AAA) yijCDNA‑binding transcriptional repressor
*REL6061,176,0100TG26.6% 179.0 / 20.0 79A37A (GCA→GCCfhuEferric‑rhodotorulic acid outer membrane transporter
*REL6064,169,2320AT26.4% 177.3 / 22.7 72intergenic (+48/‑165)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6062,296,3290AC25.3% 178.4 / 26.5 75intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6064,322,5650TG23.3% 148.4 / 10.6 60G157G (GGT→GGGmelBmelibiose:sodium symporter
*REL6061,882,5260TG22.8% 175.7 / 10.9 79I21S (ATT→AGT) manZmannose‑specific enzyme IID component of PTS
*REL6064,574,2520TG21.3% 118.3 / 12.9 47S5S (TCA→TCChpaXhypothetical 4‑hydroxyphenylacetate permease

Marginal new junction evidence (lowest skew 10 of 17 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3180864 =91 (1.300)6 (0.090) 6/136 6.5 6.5% coding (58/663 nt) yqjA conserved inner membrane protein
?REL606 3180885 = 89 (1.400)coding (79/663 nt) yqjA conserved inner membrane protein
* ? REL606 1643503 =59 (0.840)4 (0.060)
+CATCGATGG
4/132 7.4 7.2% coding (994/1257 nt) ynfJ putative voltage‑gated ClC‑type chloride channel ClcB
?REL606 1643503 = 59 (0.840)coding (994/1257 nt) ynfJ putative voltage‑gated ClC‑type chloride channel ClcB
* ? REL606 1643503 =59 (0.840)4 (0.060) 4/138 7.6 5.3% coding (994/1257 nt) ynfJ putative voltage‑gated ClC‑type chloride channel ClcB
?REL606 3180870 = 89 (1.380)coding (64/663 nt) yqjA conserved inner membrane protein
* ? REL606 = 279181799 (1.410)7 (0.110) 4/142 7.7 7.1% coding (1422/1479 nt) ygcE predicted kinase
?REL606 = 2792302 89 (1.340)intergenic (+428/‑207) ygcE/ECB_02621 predicted kinase/conserved hypothetical protein
* ? REL606 2039188 =95 (1.360)9 (0.140) 4/140 7.7 8.0% coding (507/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039561 = 119 (1.820)coding (134/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 2036748 =93 (1.330)4 (0.060) 4/140 7.7 4.0% coding (211/1113 nt) wbbC putative glycosyltransferase WbbC
?REL606 2036931 = 105 (1.610)coding (28/1113 nt) wbbC putative glycosyltransferase WbbC
* ? REL606 1325040 =47 (0.670)4 (0.070)
+TGTTATAACA
3/130 7.9 9.0% intergenic (+29/‑184) yciQ/yciL hypothetical protein; b1268_1/23S rRNA pseudouridylate synthase
?REL606 1325040 = 47 (0.670)intergenic (+29/‑184) yciQ/yciL hypothetical protein; b1268_1/23S rRNA pseudouridylate synthase
* ? REL606 3564938 =121 (1.730)3 (0.050) 3/136 8.1 2.6% coding (210/1608 nt) yhiKL hypothetical protein
?REL606 3565315 = 111 (1.750)intergenic (‑168/‑215) yhiKL/yhiM hypothetical protein/conserved inner membrane protein
* ? REL606 3737289 =92 (1.310)3 (0.050) 3/138 8.2 3.2% coding (538/1254 nt) waaL Lipid A‑core, surface polymer ligase
?REL606 3737758 = 97 (1.510)coding (69/1254 nt) waaL Lipid A‑core, surface polymer ligase
* ? REL606 3026311 =118 (1.680)3 (0.050) 3/140 8.3 3.0% coding (492/1377 nt) ECB_02826 hypothetical protein
?REL606 3026805 = 87 (1.330)intergenic (‑3/+206) ECB_02826/ECB_02827 hypothetical protein/Polysialic acid transport ATP‑binding KpsT