breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,000,2950TG40.4% 63.5 / 19.6 48G533G (GGT→GGGubiBputative ubiquinone biosynthesis protein UbiB
*REL6062,631,7720AC38.5% 81.7 / 19.3 52intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL6061,747,9030GT36.0% 138.8 / 88.9 86A8S (GCA→TCA) ydiLhypothetical protein
*REL6062,296,3290AC35.9% 121.1 / 28.5 64intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6061,688,9900AC32.4% 71.8 / 12.2 37T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6063,492,0820TG32.4% 132.2 / 24.5 71V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6063,983,7000TG31.6% 63.9 / 19.2 38T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL606273,5810TG31.2% 167.4 / 12.2 80G136G (GGT→GGGyagUconserved inner membrane protein
*REL6061,004,1060AC27.9% 153.5 / 16.8 68intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL6061,484,1050AG26.5% 100.1 / 18.2 49G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,882,5260TG26.1% 134.6 / 10.0 69I21S (ATT→AGT) manZmannose‑specific enzyme IID component of PTS
*REL6064,396,5130CG22.9% 85.6 / 11.1 35S32S (TCG→TCCyjfQDNA‑binding transcriptional dual regulator
*REL6061,875,4830GC20.4% 262.6 / 60.6 98intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I

Marginal new junction evidence (lowest skew 10 of 56 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 203959664 (1.030)8 (0.150) 6/130 5.7 12.3% coding (99/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 = 2039906 59 (1.100)coding (223/438 nt) vioB VioB, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 2040618 =42 (0.680)8 (0.140) 6/134 5.8 18.7% coding (766/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2040858 = 32 (0.580)coding (526/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 2038724 =51 (0.820)12 (0.220) 6/134 5.8 20.3% coding (225/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 2039214 = 49 (0.890)coding (481/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 203896865 (1.050)7 (0.120) 6/136 5.9 13.1% coding (727/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 = 2039373 34 (0.610)coding (322/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 373962680 (1.290)11 (0.190) 6/140 6.0 13.1% coding (340/1026 nt) waaW UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase
?REL606 = 3739937 71 (1.230)coding (29/1026 nt) waaW UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase
* ? REL606 2041220 =51 (0.820)6 (0.100) 6/142 6.1 8.9% coding (164/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041465 = 74 (1.260)coding (1356/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 3021153 =79 (1.270)7 (0.120) 5/138 6.4 8.5% coding (331/933 nt) ECB_02820 hypothetical protein
?REL606 3021584 = 79 (1.390)coding (1101/1140 nt) ECB_02821 hypothetical protein
* ? REL606 2038236 =54 (0.870)7 (0.120) 5/136 6.4 8.8% coding (713/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 2038515 = 97 (1.730)coding (434/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 2039179 =30 (0.480)6 (0.100) 5/140 6.5 11.3% coding (516/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039571 = 66 (1.140)coding (124/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 3737261115 (1.850)6 (0.100) 5/142 6.6 6.1% coding (566/1254 nt) waaL Lipid A‑core, surface polymer ligase
?REL606 = 3737695 76 (1.290)coding (132/1254 nt) waaL Lipid A‑core, surface polymer ligase