breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 4,000,295 | 0 | T | G | 40.4% | 63.5 / 19.6 | 48 | G533G (GGT→GGG) | ubiB | putative ubiquinone biosynthesis protein UbiB |
* | REL606 | 2,631,772 | 0 | A | C | 38.5% | 81.7 / 19.3 | 52 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 1,747,903 | 0 | G | T | 36.0% | 138.8 / 88.9 | 86 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 2,296,329 | 0 | A | C | 35.9% | 121.1 / 28.5 | 64 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 1,688,990 | 0 | A | C | 32.4% | 71.8 / 12.2 | 37 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) |
* | REL606 | 3,492,082 | 0 | T | G | 32.4% | 132.2 / 24.5 | 71 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 3,983,700 | 0 | T | G | 31.6% | 63.9 / 19.2 | 38 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 273,581 | 0 | T | G | 31.2% | 167.4 / 12.2 | 80 | G136G (GGT→GGG) | yagU | conserved inner membrane protein |
* | REL606 | 1,004,106 | 0 | A | C | 27.9% | 153.5 / 16.8 | 68 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
* | REL606 | 1,484,105 | 0 | A | G | 26.5% | 100.1 / 18.2 | 49 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,882,526 | 0 | T | G | 26.1% | 134.6 / 10.0 | 69 | I21S (ATT→AGT) | manZ | mannose‑specific enzyme IID component of PTS |
* | REL606 | 4,396,513 | 0 | C | G | 22.9% | 85.6 / 11.1 | 35 | S32S (TCG→TCC) | yjfQ | DNA‑binding transcriptional dual regulator |
* | REL606 | 1,875,483 | 0 | G | C | 20.4% | 262.6 / 60.6 | 98 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
Marginal new junction evidence (lowest skew 10 of 56 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 2039596 | 64 (1.030) | 8 (0.150) | 6/130 | 5.7 | 12.3% | coding (99/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | = 2039906 | 59 (1.100) | coding (223/438 nt) | vioB | VioB, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | 2040618 = | 42 (0.680) | 8 (0.140) | 6/134 | 5.8 | 18.7% | coding (766/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2040858 = | 32 (0.580) | coding (526/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | 2038724 = | 51 (0.820) | 12 (0.220) | 6/134 | 5.8 | 20.3% | coding (225/822 nt) | wbbB | putative glycosyltransferase WbbB |
? | REL606 | 2039214 = | 49 (0.890) | coding (481/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | = 2038968 | 65 (1.050) | 7 (0.120) | 6/136 | 5.9 | 13.1% | coding (727/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | = 2039373 | 34 (0.610) | coding (322/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | = 3739626 | 80 (1.290) | 11 (0.190) | 6/140 | 6.0 | 13.1% | coding (340/1026 nt) | waaW | UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase |
? | REL606 | = 3739937 | 71 (1.230) | coding (29/1026 nt) | waaW | UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase | |||||
* | ? | REL606 | 2041220 = | 51 (0.820) | 6 (0.100) | 6/142 | 6.1 | 8.9% | coding (164/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2041465 = | 74 (1.260) | coding (1356/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | 3021153 = | 79 (1.270) | 7 (0.120) | 5/138 | 6.4 | 8.5% | coding (331/933 nt) | ECB_02820 | hypothetical protein |
? | REL606 | 3021584 = | 79 (1.390) | coding (1101/1140 nt) | ECB_02821 | hypothetical protein | |||||
* | ? | REL606 | 2038236 = | 54 (0.870) | 7 (0.120) | 5/136 | 6.4 | 8.8% | coding (713/822 nt) | wbbB | putative glycosyltransferase WbbB |
? | REL606 | 2038515 = | 97 (1.730) | coding (434/822 nt) | wbbB | putative glycosyltransferase WbbB | |||||
* | ? | REL606 | 2039179 = | 30 (0.480) | 6 (0.100) | 5/140 | 6.5 | 11.3% | coding (516/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | 2039571 = | 66 (1.140) | coding (124/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | = 3737261 | 115 (1.850) | 6 (0.100) | 5/142 | 6.6 | 6.1% | coding (566/1254 nt) | waaL | Lipid A‑core, surface polymer ligase |
? | REL606 | = 3737695 | 76 (1.290) | coding (132/1254 nt) | waaL | Lipid A‑core, surface polymer ligase |