breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 27 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.40.6% 148.3 / 107.7 70intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1936,2930T.34.6% 230.4 / 137.5 112intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH11,065,6050AT31.6% 171.7 / 76.8 117intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH1936,2970AG31.5% 247.8 / 130.9 156intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH11,065,6180AG30.6% 168.7 / 93.2 129intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH11,065,6140AG28.7% 171.7 / 80.1 122intergenic (+49/‑14)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH12,169,5770TG28.6% 217.8 / 41.9 188R661R (CGA→CGCrsxCelectron transporter RsxC
*DH13,448,5260AC28.0% 588.1 / 85.8 318intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH1613,7700CA27.9% 167.6 / 15.1 138intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,5480GT27.4% 330.2 / 61.4 198P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH12,326,2870GT27.4% 640.7 / 79.6 353intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH12,965,9060TG25.8% 175.7 / 26.0 144intergenic (+139/‑356)aqpZ/ybjEaquaporin/transporter
*DH14,122,1240CA25.6% 320.5 / 17.8 211intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH1613,5970GC24.3% 295.9 / 37.3 204intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,158,1080CG24.2% 591.0 / 46.1 235intergenic (+20/+27)slyA/slyBSlyA family transcriptional regulator/hypothetical protein
*DH1936,2781.T24.1% 142.1 / 68.7 58intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,169,7810GT22.2% 280.0 / 37.7 174A593A (GCC→GCArsxCelectron transporter RsxC
*DH12,945,5350TA21.9% 379.6 / 38.3 232P787P (CCA→CCTftsKcell division protein FtsK
*DH13,448,5200AC21.7% 745.5 / 48.5 318intergenic (‑80/+76)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH12,945,5540GT21.5% 450.2 / 17.1 220P781Q (CCG→CAG) ftsKcell division protein FtsK

Marginal new junction evidence (lowest skew 10 of 37 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2668875276 (0.790)89 (0.280) 76/268 9.0 27.9% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 214 (0.680)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =244 (0.770)64 (0.200) 54/268 14.3 20.9% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 = 1355505348 (0.990)47 (0.150) 31/260 21.7 17.1% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 156 (0.510)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 2590941297 (0.850)57 (0.190) 30/260 22.1 25.3% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 80 (0.260)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 3183985 =358 (1.020)62 (0.190)
+GATTGTTTCTTC
31/276 22.9 22.9% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 97 (0.280)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 1355843 =88 (0.250)85 (0.240)
+C
30/298 25.0 25.5% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3100565 413 (1.180)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355843 =88 (0.250)98 (0.280)
+C
30/298 25.0 38.7% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 225 (0.640)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355843 =88 (0.250)173 (0.500)
+C
30/298 25.0 66.4% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 = 3970042433 (1.230)24 (0.070) 24/282 26.6 5.5% intergenic (‑128/‑86) fimA/ECDH1_RS19620 type‑1 fimbrial protein, A chain/hypothetical protein
?DH1 = 3970338 420 (1.270)intergenic (+22/+58) ECDH1_RS19620/fimE hypothetical protein/tyrosine recombinase
* ? DH1 3970034 =428 (1.220)22 (0.070) 21/282 28.2 5.1% intergenic (‑120/‑94) fimA/ECDH1_RS19620 type‑1 fimbrial protein, A chain/hypothetical protein
?DH1 3970348 = 423 (1.280)intergenic (+32/+48) ECDH1_RS19620/fimE hypothetical protein/tyrosine recombinase