breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1236,0860AG76.9% 19.0 / 3.8 13intergenic (+28/+26)ECDH1_RS01120/gorhypothetical protein/glutathione reductase
*DH13,906,8370CG33.7% 115.6 / 27.9 90intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH12,169,5770TG33.3% 211.0 / 77.1 236R661R (CGA→CGCrsxCelectron transporter RsxC
*DH13,448,5260AC29.8% 650.6 / 112.4 382intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH12,169,5480GT29.0% 434.9 / 87.7 262P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH1936,1690T.28.9% 192.9 / 80.8 78intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1936,2930T.28.4% 281.5 / 117.8 112intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,591,5130TG27.7% 245.6 / 49.3 249D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH12,965,9060TG26.3% 199.2 / 27.1 174intergenic (+139/‑356)aqpZ/ybjEaquaporin/transporter
*DH12,326,2870GT25.2% 885.5 / 101.4 456intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH12,169,7200CG25.2% 154.5 / 26.9 136E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH12,965,8930AC25.2% 232.9 / 25.7 171intergenic (+126/‑369)aqpZ/ybjEaquaporin/transporter
*DH1936,2781.T25.0% 160.2 / 77.0 60intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,169,5680CG24.9% 403.1 / 36.5 241A664A (GCG→GCCrsxCelectron transporter RsxC
*DH13,448,5230AC24.9% 836.0 / 72.6 382intergenic (‑83/+73)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH1936,2970AG24.1% 371.3 / 110.2 163intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH11,647,6690AC23.9% 197.0 / 20.3 171intergenic (+248/+125)dusC/ECDH1_RS08050tRNA dihydrouridine synthase DusC/hypothetical protein
*DH13,447,4030AC23.6% 587.3 / 61.0 298G346G (GGT→GGGmalZalpha‑glycosidase
*DH14,122,1240CA23.3% 340.2 / 21.7 238intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH1102,7310AC23.2% 674.6 / 58.6 317intergenic (‑183/+15)trmL/lldDtRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/alpha‑hydroxy‑acid oxidizing enzyme

Marginal new junction evidence (lowest skew 10 of 55 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 3183985 =444 (1.030)69 (0.170)
+GATTGTTTCTTC
32/276 32.7 20.2% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 149 (0.350)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 2590941382 (0.890)51 (0.140) 28/260 33.1 18.2% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 129 (0.350)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 1355843 =124 (0.290)98 (0.230)
+C
34/298 33.9 24.2% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3100565 493 (1.150)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355843 =124 (0.290)125 (0.290)
+C
34/298 33.9 38.3% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 282 (0.660)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355843 =124 (0.290)201 (0.470)
+C
33/298 34.5 62.0% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 = 1355505383 (0.890)44 (0.120) 25/260 34.9 13.5% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 232 (0.620)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1519281301 (0.700)37 (0.090)
+AACCTTCTAATT
27/276 35.5 11.8% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 301 (0.700)coding (787/900 nt) yfaD transposase
* ? DH1 = 1519086252 (0.590)37 (0.090) 31/300 35.7 13.2% coding (71/186 nt) ECDH1_RS07485 hypothetical protein
?DH1 1519099 = 235 (0.550)coding (58/186 nt) ECDH1_RS07485 hypothetical protein
* ? DH1 1355966 =152 (0.350)89 (0.210)
+CTC
30/294 35.7 29.5% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 283 (0.660)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355844 =127 (0.300)91 (0.210)
+C
30/298 36.1 30.9% intergenic (‑45/+2) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 283 (0.660)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys