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breseq version 0.28.1 revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 29 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | DH1 | 236,086 | 0 | A | G | 76.9% | 19.0 / 3.8 | 13 | intergenic (+28/+26) | ECDH1_RS01120/gor | hypothetical protein/glutathione reductase |
* | DH1 | 3,906,837 | 0 | C | G | 33.7% | 115.6 / 27.9 | 90 | intergenic (+8/‑27) | ECDH1_RS19315/ECDH1_RS19320 | tRNA‑Leu/tRNA‑Leu |
* | DH1 | 2,169,577 | 0 | T | G | 33.3% | 211.0 / 77.1 | 236 | R661R (CGA→CGC) | rsxC | electron transporter RsxC |
* | DH1 | 3,448,526 | 0 | A | C | 29.8% | 650.6 / 112.4 | 382 | intergenic (‑86/+70) | malZ/proY | alpha‑glycosidase/proline‑specific permease ProY |
* | DH1 | 2,169,548 | 0 | G | T | 29.0% | 434.9 / 87.7 | 262 | P671Q (CCG→CAG) | rsxC | electron transporter RsxC |
* | DH1 | 936,169 | 0 | T | . | 28.9% | 192.9 / 80.8 | 78 | intergenic (‑13/+21) | ECDH1_RS04615/ECDH1_RS04620 | tRNA‑Met/tRNA‑Met |
* | DH1 | 936,293 | 0 | T | . | 28.4% | 281.5 / 117.8 | 112 | intergenic (‑27/+7) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 2,591,513 | 0 | T | G | 27.7% | 245.6 / 49.3 | 249 | D86A (GAC→GCC) | ECDH1_RS12815 | hypothetical protein |
* | DH1 | 2,965,906 | 0 | T | G | 26.3% | 199.2 / 27.1 | 174 | intergenic (+139/‑356) | aqpZ/ybjE | aquaporin/transporter |
* | DH1 | 2,326,287 | 0 | G | T | 25.2% | 885.5 / 101.4 | 456 | intergenic (+207/+200) | yddM/fdnI | transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit |
* | DH1 | 2,169,720 | 0 | C | G | 25.2% | 154.5 / 26.9 | 136 | E614Q (GAA→CAA) | rsxC | electron transporter RsxC |
* | DH1 | 2,965,893 | 0 | A | C | 25.2% | 232.9 / 25.7 | 171 | intergenic (+126/‑369) | aqpZ/ybjE | aquaporin/transporter |
* | DH1 | 936,278 | 1 | . | T | 25.0% | 160.2 / 77.0 | 60 | intergenic (‑12/+22) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 2,169,568 | 0 | C | G | 24.9% | 403.1 / 36.5 | 241 | A664A (GCG→GCC) | rsxC | electron transporter RsxC |
* | DH1 | 3,448,523 | 0 | A | C | 24.9% | 836.0 / 72.6 | 382 | intergenic (‑83/+73) | malZ/proY | alpha‑glycosidase/proline‑specific permease ProY |
* | DH1 | 936,297 | 0 | A | G | 24.1% | 371.3 / 110.2 | 163 | intergenic (‑31/+3) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 1,647,669 | 0 | A | C | 23.9% | 197.0 / 20.3 | 171 | intergenic (+248/+125) | dusC/ECDH1_RS08050 | tRNA dihydrouridine synthase DusC/hypothetical protein |
* | DH1 | 3,447,403 | 0 | A | C | 23.6% | 587.3 / 61.0 | 298 | G346G (GGT→GGG) | malZ | alpha‑glycosidase |
* | DH1 | 4,122,124 | 0 | C | A | 23.3% | 340.2 / 21.7 | 238 | intergenic (‑15/+22) | ECDH1_RS20430/ECDH1_RS20435 | tRNA‑Gly/tRNA‑Gly |
* | DH1 | 102,731 | 0 | A | C | 23.2% | 674.6 / 58.6 | 317 | intergenic (‑183/+15) | trmL/lldD | tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/alpha‑hydroxy‑acid oxidizing enzyme |
Marginal new junction evidence (lowest skew 10 of 55 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | DH1 | 3183985 = | 444 (1.030) | 69 (0.170) +GATTGTTTCTTC |
32/276 | 32.7 | 20.2% | intergenic (+379/‑1) | asnB/ECDH1_RS15770 | asparagine synthetase B/tRNA‑Met |
? | DH1 | = 3184256 | 149 (0.350) | intergenic (+2/‑33) | ECDH1_RS15780/ECDH1_RS15785 | tRNA‑Gln/tRNA‑Gln | |||||
* | ? | DH1 | = 2590941 | 382 (0.890) | 51 (0.140) | 28/260 | 33.1 | 18.2% | intergenic (+1/‑209) | ECDH1_RS12805/ECDH1_RS12810 | tRNA‑Tyr/tRNA‑Tyr |
? | DH1 | 2591414 = | 129 (0.350) | noncoding (199/555 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | 1355843 = | 124 (0.290) | 98 (0.230) +C |
34/298 | 33.9 | 24.2% | intergenic (‑44/+3) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3100565 | 493 (1.150) | noncoding (1/76 nt) | ECDH1_RS15385 | tRNA‑Lys | |||||
* | ? | DH1 | 1355843 = | 124 (0.290) | 125 (0.290) +C |
34/298 | 33.9 | 38.3% | intergenic (‑44/+3) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3099750 | 282 (0.660) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | 1355843 = | 124 (0.290) | 201 (0.470) +C |
33/298 | 34.5 | 62.0% | intergenic (‑44/+3) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3099542 | NA (NA) | noncoding (268/268 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | = 1355505 | 383 (0.890) | 44 (0.120) | 25/260 | 34.9 | 13.5% | noncoding (54/348 nt) | repeat_region | repeat region |
? | DH1 | 3099693 = | 232 (0.620) | noncoding (58/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | = 1519281 | 301 (0.700) | 37 (0.090) +AACCTTCTAATT |
27/276 | 35.5 | 11.8% | coding (788/900 nt) | yfaD | transposase |
? | DH1 | 1519282 = | 301 (0.700) | coding (787/900 nt) | yfaD | transposase | |||||
* | ? | DH1 | = 1519086 | 252 (0.590) | 37 (0.090) | 31/300 | 35.7 | 13.2% | coding (71/186 nt) | ECDH1_RS07485 | hypothetical protein |
? | DH1 | 1519099 = | 235 (0.550) | coding (58/186 nt) | ECDH1_RS07485 | hypothetical protein | |||||
* | ? | DH1 | 1355966 = | 152 (0.350) | 89 (0.210) +CTC |
30/294 | 35.7 | 29.5% | noncoding (76/76 nt) | ECDH1_RS06685 | tRNA‑Val |
? | DH1 | = 3099750 | 283 (0.660) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | 1355844 = | 127 (0.300) | 91 (0.210) +C |
30/298 | 36.1 | 30.9% | intergenic (‑45/+2) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3099750 | 283 (0.660) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys |