breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,1970GT37.0% 114.4 / 15.2 80intergenic (‑32/+3)leuV/leuPtRNA‑Leu/tRNA‑Leu
*NC_000913422,4290TG33.3% 616.2 / 132.2 402intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009131,708,9300CA30.1% 376.0 / 84.1 238P671Q (CCG→CAG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009133,269,9940GT28.8% 191.3 / 24.3 176intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009134,296,0600CT28.2% 725.8 / inf 612intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009131,708,8800CA27.7% 277.7 / 35.6 184A654A (GCC→GCArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009133,270,1670CG27.5% 304.0 / 57.2 243intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009131,708,7580GC27.3% 137.7 / 40.4 136E614Q (GAA→CAA) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,947,5910A.26.9% 279.3 / 101.8 105intergenic (+18/‑16)metW/metVtRNA‑Met/tRNA‑Met
*NC_0009133,270,1630CA26.4% 320.3 / 16.1 231intergenic (+561/+462)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009132,560,9960GT26.3% 587.2 / 70.0 343intergenic (+98/‑372)yffL/yffMCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
*NC_0009131,552,1910CA26.0% 1011.9 / 112.9 490intergenic (+200/+207)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
*NC_0009133,270,1510AC25.5% 205.1 / 40.6 212intergenic (+549/+474)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009131,286,9650AC25.4% 190.9 / 32.7 216intergenic (+439/+122)narI/tprnitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein
*NC_0009131,708,9010AC25.4% 256.3 / 41.4 220R661R (CGA→CGCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,947,4670AC24.8% 278.5 / 112.8 129intergenic (+4/‑30)metZ/metWtRNA‑Met/tRNA‑Met
*NC_000913422,4320TG24.7% 838.1 / 75.8 402intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009131,708,8620CA24.5% 225.4 / 12.8 163V648V (GTC→GTArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,947,4650T.24.5% 391.3 / 131.9 152intergenic (+2/‑32)metZ/metWtRNA‑Met/tRNA‑Met
*NC_0009132,818,1580TA24.3% 339.9 / 105.0 203intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg

Marginal new junction evidence (lowest skew 10 of 33 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =365 (0.800)89 (0.220) 74/268 16.7 21.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 329 (0.800)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805345 (0.750)90 (0.220) 72/268 17.3 22.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 313 (0.770)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 696863 =146 (0.320)109 (0.260)
+GAAGAAACAATC
44/276 28.4 27.9% intergenic (‑33/+2) glnW/glnU tRNA‑Gln/tRNA‑Gln
?NC_000913 = 697134 466 (1.020)intergenic (‑1/+379) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 781446344 (0.750)48 (0.120) 34/260 31.3 12.7% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 2521487 = 361 (0.910)intergenic (+159/+106) lysV/xapR tRNA‑Lys/transcriptional activator of xapAB
* ? NC_000913 781369 =339 (0.740)128 (0.280)
+G
32/298 36.5 36.7% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521129 106 (0.230)intergenic (+3/‑44) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 781577 =342 (0.750)199 (0.440)
+G
31/298 37.0 47.2% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521129 106 (0.230)intergenic (+3/‑44) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 780554 =485 (1.060)96 (0.210)
+G
31/298 37.0 24.6% noncoding (1/76 nt) lysT tRNA‑Lys
?NC_000913 = 2521129 106 (0.230)intergenic (+3/‑44) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 = 1287084149 (0.330)41 (0.100) 20/260 39.6 16.1% intergenic (+558/+3) narI/tpr nitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein
?NC_000913 1287557 = 300 (0.760)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 781369 =339 (0.740)93 (0.210)
+GAG
25/294 40.0 26.2% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 195 (0.430)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781369 =339 (0.740)108 (0.240)
+G
25/298 40.4 32.7% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521128 109 (0.240)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val