breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 402 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,101,2480AG74.5% 156.7 / 55.8 94P226P (CCT→CCCcsgGouter membrane lipoprotein
*W3110S.gb2,696,4300CT74.3% 118.4 / 42.4 74V39V (GTG→GTAtadAtRNA‑specific adenosine deaminase
*W3110S.gb2,696,4540TC74.3% 123.9 / 40.8 74K31K (AAA→AAGtadAtRNA‑specific adenosine deaminase
*W3110S.gb1,487,1440TG74.3% 194.7 / 60.0 101V771V (GTT→GTGhrpAATP‑dependent helicase
*W3110S.gb3,724,5470TA74.3% 180.5 / 68.4 101I511I (ATT→ATAglmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
*W3110S.gb2,007,7690AG74.0% 171.5 / 63.1 104E110E (GAA→GAGfliSflagellar protein potentiates polymerization
*W3110S.gb1,157,0180GA73.6% 111.1 / 43.8 72Q106Q (CAG→CAAtmkthymidylate kinase
*W3110S.gb1,157,0210GC73.6% 121.2 / 49.9 72A107A (GCG→GCCtmkthymidylate kinase
*W3110S.gb3,186,2931.T73.3% 30.8 / 10.0 15coding (238/2466 nt)yqiGpredicted outer membrane usher protein
*W3110S.gb3,937,9981.A73.3% 30.3 / 10.4 15coding (450/1005 nt)dppFdipeptide transporter
*W3110S.gb823,1150TC73.2% 86.6 / 33.0 56E349E (GAA→GAGybhOcardiolipin synthase 2
*W3110S.gb36,3850AG73.1% 77.4 / 30.4 52F485F (TTT→TTCcaiCpredicted crotonobetaine CoA ligase:carnitine CoA ligase
*W3110S.gb3,323,5210CT73.0% 109.5 / 46.9 74K135K (AAG→AAAglmMphosphoglucosamine mutase
*W3110S.gb2,118,8570GT72.9% 144.6 / 130.2 151L136L (CTC→CTAwcaLpredicted glycosyl transferase
*W3110S.gb1,713,9880GA72.7% 61.7 / 26.1 44intergenic (+116/‑495)nth/ydgRDNA glycosylase and apyrimidinic (AP) lyase/predicted transporter
*W3110S.gb3,172,6240GA72.6% 156.6 / 70.6 106L477L (CTG→TTG) parEDNA topoisomerase IV, subunit B
*W3110S.gb3,323,5540CT72.6% 108.6 / 46.5 73A124A (GCG→GCAglmMphosphoglucosamine mutase
*W3110S.gb486,7970TA72.5% 105.2 / 51.4 69S346S (TCT→TCAkefAfused mechanosensitive channel proteins
*W3110S.gb2,794,2250AT72.4% 90.3 / 40.8 58I439I (ATA→ATTgabPgamma‑aminobutyrate transporter
*W3110S.gb2,007,7870CT72.4% 155.1 / 70.4 105R116R (CGC→CGTfliSflagellar protein potentiates polymerization

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 275643613 (0.400)7 (0.240)
+TTATTT
3/100 0.1 7.9% coding (821/957 nt) yfjH hypothetical protein
?W3110S.gb 4484645 = 169 (5.230)coding (234/1197 nt) yjgN conserved inner membrane protein