breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA CP006881 30,182 C→T 100% intergenic (+147/‑96) U712_00120 → / → U712_20865 Sigma‑K factor‑processing regulatory protein BofA/16S ribosomal RNA
RA CP006881 35,960 A→G 10.9% Y39C (TAT→TGT)  U712_00130 → Protein xpaC
RA CP006881 53,413 G→A 100% intergenic (+45/‑94) U712_00230 → / → U712_00235 Protein sspF/4‑diphosphocytidyl‑2‑C‑methyl‑D‑erythritol kinase
RA CP006881 214,982 C→T 100% A268V (GCC→GTC)  U712_00980 → Uncharacterized protein skfB
RA CP006881 249,190 A→G 13.7% H146R (CAT→CGT)  U712_01165 → Phosphatidylserine decarboxylase proenzyme
RA CP006881 454,851 A→G 7.0% Y134Y (TAT→TAC U712_02090 ← Uncharacterized protein YczH
RA CP006881 660,041 C→A 5.7% intergenic (+9/‑150) U712_03220 → / → U712_03225 Uncharacterized protein yebC/Uncharacterized protein yebD
RA CP006881 916,340 C→A 9.8% intergenic (+12/+67) U712_21235 → / ← U712_04455 tRNA‑Leu/Sporulation‑control protein spo0M
RA CP006881 924,066:1 +A 100% intergenic (‑46/‑233) U712_04490 ← / → U712_04495 Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB
RA CP006881 1,051,313 T→A 7.8% I28N (ATC→AAC)  U712_05170 → Protoporphyrinogen oxidase
RA CP006881 1,091,483:1 +T 100% coding (1163/1326 nt) U712_05390 ← NTD biosynthesis operon protein ntdA
RA CP006881 1,105,789 A→G 16.8% P983P (CCA→CCG U712_05435 → ATP‑dependent helicase/nuclease subunit A
RA CP006881 1,211,770 A→G 14.4% A22A (GCA→GCG U712_06040 → Spore coat protein O
RA CP006881 1,219,596 A→G 6.0% A99A (GCT→GCC U712_06110 ← putative N‑acetyltransferase YjcF
RA CP006881 1,653,491 C→T 19.4% T112I (ACA→ATA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,712 G→A 37.7% A186T (GCA→ACA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,890 T→C 35.0% L245P (CTA→CCA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,655,978 Δ1 bp 35.5% coding (430/1596 nt) U712_08515 → Flagellar M‑ring protein
JC CP006881 1,659,332 Δ141 bp 22.4% coding (418‑558/1317 nt) U712_08530 → Flagellum‑specific ATP synthase
JC CP006881 1,662,121 (AGCTGAAAGCC)2→1 6.6% coding (806‑816/1464 nt) U712_08545 → putative flagellar hook‑length control protein
RA CP006881 1,662,223 C→A 17.1% S303* (TCG→TAG)  U712_08545 → putative flagellar hook‑length control protein
RA CP006881 1,663,209 G→T 9.0% intergenic (+11/‑11) U712_08550 → / → U712_08555 FlaA locus uncharacterized protein ylxG/Flagellar basal‑body rod protein flgG
RA CP006881 2,249,253 C→A 5.5% intergenic (‑42/+352) U712_11310 ← / ← U712_11315 Riboflavin biosynthesis protein RibD/Uncharacterized protein ypuD
RA CP006881 2,256,420 T→C 8.4% T434T (ACA→ACG U712_11370 ← Stage V sporulation protein AF
RA CP006881 2,289,100 G→T 100% P184T (CCG→ACG)  U712_11585 ← putative oxidoreductase yqjQ
RA CP006881 2,658,622 C→A 11.0% intergenic (+11/+35) U712_13680 → / ← U712_13685 Putative serine/threonine‑protein kinase yrzF/Hypothetical Protein
RA CP006881 2,801,996 G→A 100% A327T (GCC→ACC)  U712_14440 → UPF0118 membrane protein ytvI
RA CP006881 2,896,953 T→C 22.1% K72E (AAG→GAG)  U712_14940 ← Uncharacterized protein yteU
RA CP006881 2,955,315 C→A 10.1% intergenic (‑85/+35) U712_15230 ← / ← U712_15235 Uncharacterized protein ytkA/S‑ribosylhomocysteine lyase
RA CP006881 2,963,937 G→A 5.9% intergenic (‑124/+156) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
RA CP006881 2,963,941 A→G 34.9% intergenic (‑128/+152) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
RA CP006881 2,963,948 G→A 41.7% intergenic (‑135/+145) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
RA CP006881 3,010,076 A→G 5.0% L386P (CTG→CCG)  U712_15465 ← putative MFS‑type transporter yubD
RA CP006881 3,285,257:1 +T 100% intergenic (‑77/+143) U712_16905 ← / ← U712_16910 Transcriptional repressor sdpR/Glycine betaine/carnitine/choline transport system permease protein opuCD
RA CP006881 3,350,164 G→T 7.0% intergenic (+12/+15) U712_17215 → / ← U712_17220 Hypothetical Protein/Phenolic acid decarboxylase padC
RA CP006881 3,438,133 T→A 8.7% I439K (ATA→AAA)  U712_17650 → putative MFS‑type transporter yvkA
RA CP006881 3,578,466 Δ1 bp 100% intergenic (‑59/‑76) U712_18365 ← / → U712_18370 Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH
RA CP006881 3,579,785 Δ1 bp 100% coding (73/132 nt) U712_18380 → Hypothetical Protein
RA CP006881 3,581,989 A→T 21.9% *258K (TAA→AAA)  U712_18400 ← HTH‑type transcriptional activator mta
RA CP006881 3,618,134 C→T 17.3% G20S (GGT→AGT)  U712_18650 ← putative transporter YwkB
RA CP006881 3,723,022 G→A 100% intergenic (‑136/‑165) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,045 T→C 100% intergenic (‑159/‑142) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,066:1 +A 89.0% intergenic (‑180/‑121) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,067 G→T 100% intergenic (‑181/‑120) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,091 A→T 100% intergenic (‑205/‑96) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,095 T→A 100% intergenic (‑209/‑92) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,097 T→G 100% intergenic (‑211/‑90) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,118 C→T 100% intergenic (‑232/‑69) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,121 T→A 100% intergenic (‑235/‑66) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,130 A→T 100% intergenic (‑244/‑57) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,162 C→T 100% intergenic (‑276/‑25) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,166 C→G 100% intergenic (‑280/‑21) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,183 A→G 100% intergenic (‑297/‑4) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,204 G→A 100% L6L (TTG→TTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,210 A→G 100% K8K (AAA→AAG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,222 C→T 100% Y12Y (TAC→TAT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,231 C→A 100% N15K (AAC→AAA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,236 T→A 100% L17Q (CTA→CAA)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,246 T→C 100% F20F (TTT→TTC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,248 T→C 100% V21A (GTT→GCT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,252 T→A 100% S22S (TCT→TCA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,273 2 bp→TC 100% coding (87‑88/771 nt) U712_19195 → putative transporter ywcJ
RA CP006881 3,723,276 A→G 100% R30R (AGA→CGG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,291 C→T 100% S35S (AGC→AGT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,294 G→A 100% M36I (ATG→ATA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,300 C→T 100% I38I (ATC→ATT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,303 A→T 100% G39G (GGA→GGT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,306 C→T 100% F40F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,336 T→C 100% S50S (AGT→AGC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,344 C→T 100% S53F (TCT→TTT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,351 G→T 100% A55A (GCG→GCT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,366 T→C 100% A60A (GCT→GCC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,387 A→T 100% T67T (ACA→ACT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,406 C→A 100% L74M (CTG→ATG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,438 T→C 100% T84T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,447 T→C 100% T87T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,459 T→C 100% T91T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,474 A→G 100% R96R (CGA→CGG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,483 2 bp→CA 100% coding (297‑298/771 nt) U712_19195 → putative transporter ywcJ
RA CP006881 3,723,492 A→C 100% R102R (CGA→CGC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,501 G→A 100% L105L (CTG→CTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,505 C→T 100% L107L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,534 G→A 100% L116L (TTG→TTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,549 A→G 100% L121L (CTA→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,558 T→C 100% I124I (ATT→ATC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,573 A→G 100% T129T (ACA→ACG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,576 G→A 100% G130G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,589 G→C 100% A135P (GCT→CCT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,616 C→T 100% L144L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,642 C→G 100% P152P (CCC→CCG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,652 C→T 100% L156L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,666 G→A 100% G160G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,717 T→C 100% L177L (CTT→CTC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,729 G→A 100% G181G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,738 C→T 100% L184L (CTC→CTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,771 C→T 100% F195F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,783 C→T 100% F199F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,822 A→G 100% S212S (TCA→TCG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,853 G→C 100% V223M (GTA→CTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,855 A→G 100% V223M (GTA→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,879 T→C 100% I231I (ATT→ATC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,888 A→G 100% T234T (ACA→ACG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,897 C→T 100% N237N (AAC→AAT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,912 G→A 100% M242I (ATG→ATA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,915 C→T 100% V243V (GTC→GTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,933 T→C 100% Y249Y (TAT→TAC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,939 G→A 100% T251T (ACG→ACA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,942 C→G 100% L252L (CTC→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,948 C→T 100% P254P (CCC→CCT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,952 A→C 100% K256Q (AAA→CAA)  U712_19195 → putative transporter ywcJ
RA CP006881 3,724,053 C→T 100% E258E (GAG→GAA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,078 C→T 100% S250N (AGT→AAT)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,092 T→C 100% A245A (GCA→GCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,101 A→C 100% L242L (CTT→CTG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,119 A→G 100% F236F (TTT→TTC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,194 A→G 100% S211S (AGT→AGC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,200 T→C 100% A209A (GCA→GCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,208 T→G 100% R207R (AGA→CGA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,274 C→T 100% E185K (GAA→AAA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,392 A→C 100% L145L (CTT→CTG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,446 G→A 100% Y127Y (TAC→TAT U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,467 T→C 100% K120K (AAA→AAG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,542 A→G 100% S95S (TCT→TCC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,587 C→T 100% G80G (GGG→GGA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,623 A→T 100% I68I (ATT→ATA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,659 A→G 100% F56F (TTT→TTC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,678 T→C 100% H50R (CAC→CGC)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,719 G→A 100% D36D (GAC→GAT U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,749 T→C 100% P26P (CCA→CCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,808 G→A 100% L7L (CTA→TTA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,831 C→T 100% intergenic (‑5/+35) U712_19200 ← / ← U712_19205 SacPA operon antiterminator/Uncharacterized protein ywcI
RA CP006881 3,724,849 A→T 100% intergenic (‑23/+17) U712_19200 ← / ← U712_19205 SacPA operon antiterminator/Uncharacterized protein ywcI
RA CP006881 3,724,902 A→C 100% A89A (GCT→GCG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,724,977 T→C 100% Q64Q (CAA→CAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,724,980 T→C 100% K63K (AAA→AAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,010 C→T 100% E53E (GAG→GAA U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,025 A→G 100% L48L (CTT→CTC U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,031 C→T 100% A46A (GCG→GCA U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,043 C→A 100% A42A (GCG→GCT U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,049 T→C 100% K40K (AAA→AAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,082 A→C 100% A29A (GCT→GCG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,205:1 +T 100% intergenic (‑37/‑492) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,725,237 A→G 100% intergenic (‑69/‑460) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,725,239 2 bp→AA 100% intergenic (‑71/‑457) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,767,782 C→A 9.6% intergenic (+8/+22) U712_19420 → / ← U712_19425 Aminopeptidase YwaD/GTP pyrophosphokinase ywaC
RA CP006881 3,772,635 A→G 11.3% Y334C (TAT→TGT)  U712_19455 → Protein dltB
RA CP006881 3,831,557 G→A 28.0% intergenic (‑6/+90) U712_19755 ← / ← U712_19760 Transcription antiterminator LicT/Uncharacterized protein yxiP
RA pSIJ663_pHT315_araE_xy 2,146 A→G 16.2% intergenic (–/‑832)  / → eryR –/–
RA pSIJ663_pHT315_araE_xy 2,214 C→T 44.5% intergenic (–/‑764)  / → eryR –/–
JC pSIJ663_pHT315_araE_xy 2,655 Δ3 bp 100% intergenic (–/‑321)  / → eryR –/–
JC pSIJ663_pHT315_araE_xy 3,843 Δ5 bp 100% intergenic (+128/‑24) eryR → / → ampR –/–
RA pSIJ663_pHT315_araE_xy 5,049 C→T 100% intergenic (+318/–) ampR → /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP006881 166409–171323 176266–171335 13–9858 215 [213] [211] 218 U712_21020–[U712_21045] U712_21020,U712_21025,U712_21030,U712_21035,U712_21040,[U712_21045]
* * ÷ CP006881 245133 246160 1028 214 [211] [210] 215 U712_01140–[U712_01145] U712_01140,[U712_01145]
* * ÷ CP006881 1067390 1068669 1280 215 [213] [212] 216 [U712_05245]–U712_05250 [U712_05245],U712_05250
* * ÷ CP006881 1148992 1150758 1767 216 [212] [213] 215 U712_05685–[U712_05690] U712_05685,[U712_05690]
* * ÷ CP006881 1503013 1504706 1694 215 [213] [210] 215 [U712_07735]–U712_07740 [U712_07735],U712_07740
* * ÷ CP006881 1562256 1566685 4430 217 [213] [211] 215 U712_08060 U712_08060
* * ÷ CP006881 3302007–3303322 3304612 1291–2606 215 [209] [0] 373 [U712_16990]–[U712_16995] [U712_16990],[U712_16995]
* * ÷ CP006881 3725631 3728180 2550 214 [213] [209] 215 U712_19210–[U712_19215] U712_19210,[U712_19215]
* * ÷ CP006881 3757658 3759729 2072 214 [211] [213] 217 U712_19375 U712_19375

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP006881 3304613 =0 (0.000)235 (0.040)
+50 bp
122/178 NT 97.9% intergenic (+1/+48) U712_16995/U712_17000 Arabinose metabolism transcriptional repressor/Uncharacterized protein yvbT
?pSIJ663_pHT315_araE_xy 9781 = 16 (0.000)intergenic (+5050/–) ampR/– –/–
* ? CP006881 = 3617989356 (1.000)30 (0.080) 28/278 NT 7.5% coding (203/960 nt) U712_18650 putative transporter YwkB
?CP006881 3618570 = 385 (1.080)coding (1401/1698 nt) U712_18655 putative NAD‑dependent malic enzyme 2
* ? pSIJ663_pHT315_araE_xy = 555117 (0.000)25314 (1.560) 269/268 NT 99.9% intergenic (+820/–) ampR/– –/–
?pSIJ663_pHT315_araE_xy 9889 = NA (NA)intergenic (+5158/–) ampR/– –/–