breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 125,085 Δ1 bp 100% coding (2069/2664 nt) aceE → pyruvate dehydrogenase E1 component
RA 192,963 G→A 15.0% R31H (CGT→CAT)  frr → ribosome‑recycling factor
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
JC JC 568,268 IS1 (–) +9 bp 100% intergenic (+21/‑39) renD → / → emrE DLP12 prophage; protein RenD/multidrug/betaine/choline efflux transporter EmrE
RA 756,792 G→T 100% E296* (GAA→TAA)  sdhA → succinate:quinone oxidoreductase, FAD binding protein
RA 1,196,220 C→T 100% H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T 100% T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C 100% L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G 100% L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,466,201 T→C 14.2% pseudogene (810/2518 nt) ydbA → putative outer membrane protein, N‑terminal fragment
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,489,152 C→T 100% S6N (AGC→AAC)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,579,023 C→A 14.1% Q120K (CAA→AAA)  talA → transaldolase A
RA 2,661,793 C→A 51.4% L113F (TTG→TTT iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,661,815 G→A 19.6% T106I (ACC→ATC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,398 T→G 100% L29R (CTT→CGT)  ygfZ → folate‑binding protein
RA 3,389,186 T→C 100% N51S (AAC→AGC)  aaeX ← DUF1656 domain‑containing protein AaeX
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,886,756 G→A 5.0% intergenic (+34/‑72) yidC → / → mnmE membrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
RA 3,886,757 T→C 5.7% intergenic (+35/‑71) yidC → / → mnmE membrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
RA 3,954,273 G→T 8.4% E266* (GAA→TAA) ‡ ilvD → dihydroxy‑acid dehydratase
RA 3,954,274 A→C 7.2% E266A (GAA→GCA) ‡ ilvD → dihydroxy‑acid dehydratase
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,049,989 A→C 10.8% noncoding (91/109 nt) spf → small regulatory RNA Spot 42
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,287,423:1 +T 100% intergenic (‑52/‑341) acs ← / → nrfA acetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase
RA 4,296,060 C→T 27.7% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,439,845 T→A 8.3% intergenic (+52/+27) ytfK → / ← ytfL DUF1107 domain‑containing protein YtfK/putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196269 1211448 15180 9 [8] [7] 9 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3621196–3622308 3653129–3652031 29724–31934 10 [8] [8] 10 [rhsB]–[insH‑11] [rhsB],yhhH,yhhI,yhhJ,rbbA,yhiI,yhiJ,yhiL,yhiM,yhiN,pitA,uspB,uspA,dtpB,rsmJ,prlC,rlmJ,gor,dinQ,agrA,agrB,arsR,arsB,arsC,yhiS,[insH‑11]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 171280325 (0.780)3 (0.100) 3/246 NT 10.9% coding (35/1503 nt) dtpA dipeptide/tripeptide:H(+) symporter DtpA
?NC_000913 = 1712811 26 (0.870)coding (43/1503 nt) dtpA dipeptide/tripeptide:H(+) symporter DtpA
* ? NC_000913 4389302 =57 (1.770)3 (0.100) 3/254 NT 5.8% coding (69/3324 nt) mscM miniconductance mechanosensitive channel MscM
?NC_000913 4389328 = 43 (1.390)coding (43/3324 nt) mscM miniconductance mechanosensitive channel MscM