breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 806,654 G→T L210M (CTG→ATG)  ybhC ← outer membrane lipoprotein YhbC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,994,427 C→A V94F (GTT→TTT)  uvrC ← excision nuclease subunit C
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,268,638 C→A G270G (GGC→GGA yeiR → zinc‑binding GTPase YeiR
RA 2,573,227 C→A P93P (CCG→CCT eutD ← phosphate acetyltransferase EutD
RA 2,841,042 A→G Q18R (CAG→CGG)  ascB → 6‑phospho‑beta‑glucosidase AscB
RA 3,005,245 C→A R181L (CGG→CTG)  ygeV ← putative sigma(54)‑dependent transcriptional regulator YgeV
RA 3,020,348 C→A G396G (GGC→GGA ssnA → putative aminohydrolase
RA 3,057,387 G→T H342N (CAC→AAC)  serA ← phosphoglycerate dehydrogenase
RA 3,075,476 T→A Q236L (CAG→CTG)  yggF ← fructose 1,6‑bisphosphatase YggF
RA 3,097,077 C→A P133T (CCG→ACG)  rdgB → dITP/XTP pyrophosphatase
RA 3,184,476 (A)7→8 intergenic (‑10/‑364) ribB ← / → ubiK 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK
RA 3,332,531 G→T P69T (CCA→ACA)  yhbE ← inner membrane protein YhbE
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,358,782 C→A S1353Y (TCC→TAC)  gltB → glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,594,825 G→T P219Q (CCG→CAG)  livM ← branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,919,330 G→T T176T (ACC→ACA atpA ← ATP synthase F1 complex subunit alpha
RA 3,931,855 G→T L180L (CTG→CTT kup → K(+):H(+) symporter Kup
RA 4,111,769 G→T S149* (TCG→TAG)  yiiQ ← DUF1454 domain‑containing protein YiiQ
RA 4,164,581 C→A Q315K (CAA→AAA)  btuB → cobalamin/cobinamide outer membrane transporter
RA 4,215,728 C→A P84H (CCT→CAT)  aceB → malate synthase A
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,277,388 C→A intergenic (‑5/‑81) soxS ← / → soxR DNA‑binding transcriptional dual regulator SoxS/DNA‑binding transcriptional dual regulator SoxR
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102950 3104069 1120 23 [22] [20] 23 mutY mutY
* * ÷ NC_000913 3423785–3424233 3424531–3424239 7–747 26 [21] [20] 24 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)109 (1.280) 64/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =130 (1.480)133 (1.520) 74/274 0.0 66.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)118 (1.340) 62/276 0.2 95.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA