breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 461,186 C→A L767M (CTG→ATG)  lon → Lon protease
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 982,195 C→A A383A (GCC→GCA ldtD → L,D‑transpeptidase LdtD
RA 1,040,245 G→T S317I (AGC→ATC)  appB → cytochrome bd‑II ubiquinol oxidase subunit II
RA 1,139,960 C→A A528E (GCG→GAG)  flgK → flagellar hook‑filament junction protein 1
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,751,107 C→A W206C (TGG→TGT ydhW ← protein YdhW
RA 1,818,449 G→T P18T (CCG→ACG)  chbF ← monoacetylchitobiose‑6‑phosphate hydrolase
RA 1,876,651 G→T A327S (GCT→TCT)  nimT → 2‑nitroimidazole exporter
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,974,054 C→A G424V (GGG→GTG)  cheA ← chemotaxis protein CheA
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,537,862 C→A F38L (TTC→TTA yfeS → conserved protein YfeS
RA 2,618,576 C→A L67F (TTG→TTT hda ← inibitor of reinitiation of DNA replication
RA 2,737,242 G→T W30L (TGG→TTG)  raiA → stationary phase translation inhibitor and ribosome stability factor
RA 2,896,127 G→T G143G (GGC→GGA ygcR ← putative flavoprotein
JC JC 2,983,961 IS5 (–) +4 bp coding (161‑164/837 nt) kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 3,139,539 G→T E271D (GAG→GAT yghU → disulfide reductase/organic hydroperoxide reductase
RA 3,179,053 G→T P313P (CCG→CCT tolC → outer membrane channel TolC
RA 3,192,579 C→A P124P (CCC→CCA yqiJ → putative inner membrane protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,726,942 C→A Q140H (CAG→CAT yiaA ← conserved inner membrane protein YiaA
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,045,403 C→A A402E (GCA→GAA)  yihF → uncharacterized protein YihF
RA 4,127,940 C→A intergenic (‑46/+138) yiiX ← / ← metJ putative lipid binding hydrolase/DNA‑binding transcriptional repressor MetJ
RA 4,197,168 C→A A89E (GCG→GAG)  nudC → NADH pyrophosphatase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,383,471 C→T H277Y (CAC→TAC)  epmA → EF‑P‑lysine lysyltransferase
RA 4,538,474 C→T E98K (GAG→AAG)  nanM ← N‑acetylneuraminate mutarotase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3103000 3104051 1052 8 [6] [7] 10 [mutY] [mutY]
* * ÷ NC_000913 3423776–3424230 3424523–3424243 14–748 9 [6] [5] 8 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =49 (1.050)32 (0.780) 27/244 0.4 42.6% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 43 (1.040)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780549 (1.050)22 (0.530) 18/244 1.0 33.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 43 (1.040)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =67 (1.430)65 (1.400) 40/274 0.1 65.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)54 (1.160) 42/276 0.1 96.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA