breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 144,849 T→A G91G (GGT→GGA yadI → putative PTS enzyme IIA component YadI
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 458,470 G→T E349* (GAA→TAA)  clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 624,859 G→T intergenic (‑349/‑26) fepB ← / → entC ferric enterobactin ABC transporter periplasmic binding protein/isochorismate synthase EntC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 974,374 A→G D19G (GAC→GGC)  mukF → chromosome partitioning protein MukF
RA 1,119,003 G→A A15V (GCG→GTG)  yceJ ← putative cytochrome b561
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,372,106 C→A P66P (CCC→CCA ycjN → putative ABC transporter periplasmic binding protein YcjN
RA 1,445,991 T→G Y38D (TAC→GAC)  ydbL → DUF1318 domain‑containing protein YdbL
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,064,892 T→A T111S (ACC→TCC)  cobS ← cobalamin 5'‑phosphate synthase
RA 2,077,851 C→A L95I (CTC→ATC)  cbtA → CP4‑44 prophage; cytoskeleton‑binding toxin CbtA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,429,334 A→T I302N (ATC→AAC)  purF ← amidophosphoribosyltransferase
RA 2,433,686 C→A D81Y (GAT→TAT)  accD ← acetyl‑CoA carboxyltransferase subunit beta
RA 2,563,831 A→C I80I (ATA→ATC yffQ → CPZ‑55 prophage; uncharacterized protein YffQ
MC JC 2,581,699 (19-bp)2→1 intergenic (+60/+17) tktB → / ← ypfG transketolase 2/DUF1176 domain‑containing protein YpfG
RA 2,594,365 C→A E499* (GAG→TAG)  tmcA ← tRNA(Met) cytidine acetyltransferase
RA 2,709,972 A→C V14G (GTC→GGC)  rpoE ← RNA polymerase sigma E factor
RA 2,731,477 C→T intergenic (‑320/+123) rrsG ← / ← clpB 16S ribosomal RNA/ClpB chaperone
RA 2,818,070 G→T noncoding (66/77 nt) argZ ← tRNA‑Arg
RA 3,023,141 C→A A609E (GCG→GAG)  xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,062,980 C→A Q711K (CAG→AAG)  scpA → methylmalonyl‑CoA mutase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,424,579 Δ1 bp noncoding (2205/2904 nt) rrlD ← 23S ribosomal RNA
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,729,171 G→A D67D (GAC→GAT xylB ← xylulokinase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196275 1211430 15156 18 [9] [13] 16 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102949 3104051 1103 16 [14] [12] 16 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424525–3424239 6–756 16 [12] [14] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)95 (1.360) 53/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =175 (2.430)105 (1.470) 59/274 0.1 54.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)107 (1.490) 60/276 0.1 98.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA