breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 211,834 C→A intergenic (+14/‑43) ldcC → / → yaeR lysine decarboxylase 2/VOC domain‑containing protein YaeR
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 772,568 C→A Q371K (CAG→AAG)  cydA → cytochrome bd‑I ubiquinol oxidase subunit I
RA 801,366 C→A A203D (GCT→GAT)  ybhH → putative isomerase YbhH
RA 812,765 G→T A166S (GCC→TCC)  bioD → dethiobiotin synthetase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,069,376 C→A G155V (GGC→GTC)  rutG ← pyrimidine:H(+) symporter
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,354,144 G→T G125G (GGC→GGA sapC ← putrescine ABC exporter membrane protein SapC
RA 1,430,448 G→T G467V (GGG→GTG)  stfR → Rac prophage; putative membrane protein
RA 1,547,660 G→T S87I (AGC→ATC)  fdnG → formate dehydrogenase N subunit alpha
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,171,455 Δ1 bp pseudogene (273/299 nt) gatR ← DNA‑binding transcriptional repressor GatR, N‑terminal fragment
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,337,694 T→C D576G (GAC→GGC)  gyrA ← DNA gyrase subunit A
RA 2,562,839 G→T A106S (GCC→TCC)  yffP → CPZ‑55 prophage; uncharacterized protein YffP
RA 2,817,985 G→T intergenic (‑125/+74) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,438,839 C→A A98S (GCC→TCC)  zntR ← DNA‑binding transcriptional activator ZntR
RA 3,479,225 A→T G461G (GGT→GGA kefB ← K(+) : H(+) antiporter KefB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,580,017 C→A P263P (CCG→CCT yhhX ← putative oxidoreductase YhhX
RA 3,678,185 G→T L56M (CTG→ATG)  yhjG ← AsmA family protein YhjG
RA 3,772,095 C→A intergenic (‑351/‑186) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,949,114 C→A G286W (GGG→TGG)  yifB ← putative magnesium chelatase YifB
RA 4,037,062 T→A noncoding (1532/1542 nt) rrsA → 16S ribosomal RNA
RA 4,085,935 C→A intergenic (‑113/‑81) fdoG ← / → fdhD formate dehydrogenase O subunit alpha/sulfurtransferase for molybdenum cofactor sulfuration
RA 4,186,763 C→A L472M (CTG→ATG)  rpoC → RNA polymerase subunit beta'
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,577,317 C→T T214T (ACG→ACA mcrC ← 5‑methylcytosine‑specific restriction enzyme subunit McrC
RA 4,618,088 Δ1 bp coding (766/780 nt) deoC → deoxyribose‑phosphate aldolase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196288 1211431 15144 9 [7] [7] 8 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102976 3104050 1075 8 [7] [7] 8 [mutY] [mutY]
* * ÷ NC_000913 3423785–3424230 3424523–3424239 10–739 8 [6] [5] 8 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =65 (1.450)79 (1.780) 48/274 0.0 70.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)39 (0.870) 30/276 0.4 92.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =27 (0.600)9 (0.220) 9/258 2.3 23.9% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 32 (0.770)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit