breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 102,587 C→A R119S (CGC→AGC)  ddlB → D‑alanine‑‑D‑alanine ligase B
RA 121,281 C→A A91S (GCC→TCC)  aroP ← aromatic amino acid:H(+) symporter AroP
RA 187,926 G→T A242E (GCA→GAA)  glnD ← PII uridylyltransferase/uridylyl removing enzyme
RA 223,514 A→G intergenic (+106/‑257) gmhB → / → rrsH D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 479,285 G→A intergenic (‑34/+82) ylaC ← / ← maa putative inner membrane protein/maltose O‑acetyltransferase
RA 736,357 T→C intergenic (+138/‑88) ybfC → / → ybfQ uncharacterized protein YbfC/inactive transposase YbfQ
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,349,168 A→G S314P (TCT→CCT)  yciW ← putative oxidoreductase
RA 1,349,854 C→A R85L (CGC→CTC)  yciW ← putative oxidoreductase
RA 1,436,833 C→A A21S (GCA→TCA)  ompN ← outer membrane porin N
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,704,711 G→T intergenic (+35/‑238) blr → / → cnu beta‑lactam resistance protein/H‑NS‑ and StpA‑binding protein
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,008,594 G→A P106P (CCG→CCA yedE → putative selenium transporter YedE
RA 2,023,929 T→C intergenic (+251/‑39) fliR → / → rcsA flagellar biosynthesis protein FliR/DNA‑binding transcriptional activator RcsA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
MC JC 2,339,172 Δ3 bp coding (247‑249/2628 nt) gyrA ← DNA gyrase subunit A
RA 2,400,815 G→T T247T (ACC→ACA nuoF ← NADH:quinone oxidoreductase subunit F
RA 2,478,871 C→A P342Q (CCG→CAG)  dsdX → D‑serine transporter
RA 2,594,589 G→T A424E (GCG→GAG)  tmcA ← tRNA(Met) cytidine acetyltransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,745,361 G→T M186I (ATG→ATT yiaN → 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,839,349 C→A V215V (GTG→GTT nlpA ← lipoprotein‑28
MC JC 3,927,532 Δ4 bp coding (378‑381/993 nt) asnA → asparagine synthetase A
RA 4,053,553 C→A intergenic (+135/+94) hemN → / ← yshB coproporphyrinogen III dehydrogenase/uncharacterized protein YshB
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,349,010 C→A intergenic (‑266/+305) dcuB ← / ← dcuR anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
RA 4,462,842 A→G intergenic (‑182/+212) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
RA 4,551,012 G→T L95L (CTC→CTA gntP ← fructuronate transporter
RA 4,552,776 C→A R381S (CGC→AGC)  uxuA → D‑mannonate dehydratase
RA 4,560,757 C→T intergenic (‑77/‑173) yjiH ← / → kptA Gate family protein YjiH/RNA 2'‑phosphotransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696780 696895 116 7 [6] [6] 7 [glnW]–[glnU] [glnW],[glnU]
* * ÷ NC_000913 1196280 1211419 15140 7 [5] [4] 7 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102971 3104043 1073 7 [6] [0] 7 [mutY] [mutY]
* * ÷ NC_000913 3423786–3424233 3424528–3424239 7–743 7 [6] [6] 7 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)64 (1.530) 51/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =79 (1.830)78 (1.820) 52/274 0.0 66.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)56 (1.300) 46/276 0.0 94.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA