breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 180,422 G→A V396I (GTC→ATC)  dgt → dGTP triphosphohydrolase
RA 251,593 C→A R232R (CGC→CGA dinB → DNA polymerase IV
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 531,122 G→T P35P (CCC→CCA selU ← tRNA 2‑selenouridine synthase
RA 774,979 C→A L76L (CTC→CTA ybgC → esterase/thioesterase
RA 780,775 C→A noncoding (11/76 nt) valT → tRNA‑Val
RA 796,327 G→T A156S (GCT→TCT)  modB → molybdate ABC transporter membrane subunit
RA 853,665 C→T R7C (CGC→TGC)  ybiR → putative transporter YbiR
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,221,708 G→T pseudogene (2108/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,225,335 C→A S9S (TCG→TCT minD ← Z‑ring positioning protein MinD
RA 1,503,276 C→A intergenic (+151/‑441) ydcL → / → insQ DUF3313 domain‑containing lipoprotein YdcL/putative insertion element transposase InsQ
JC JC 1,530,757 IS5 (–) +4 bp pseudogene (172‑175/1054 nt) yncI → putative transposase YncI
RA 1,608,834 G→T A63A (GCC→GCA yneE ← conserved inner membrane protein YneE
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,686,252 G→T R113R (CGG→AGG)  fumC ← fumarase C
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,931,446 C→A A189E (GCG→GAG)  purT → phosphoribosylglycinamide formyltransferase 2
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,011,317 T→C pseudogene (1035/1129 nt) yedN ← putative type III secreted effector, N‑terminal fragment
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,339,278 A→T V48E (GTA→GAA)  gyrA ← DNA gyrase subunit A
RA 2,420,144 C→A M114I (ATG→ATT yfcF ← glutathione S‑transferase YfcF
RA 2,578,434 C→A intergenic (‑57/‑232) maeB ← / → talA malate dehydrogenase/transaldolase A
RA 2,690,982 C→A A39A (GCG→GCT glrK ← sensory histidine kinase GlrK
RA 3,105,439 G→T R336L (CGC→CTC)  mltC → membrane‑bound lytic murein transglycosylase C
RA 3,256,565 T→C E29E (GAA→GAG cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,600,220 G→T D188E (GAC→GAA rpoH ← RNA polymerase, sigma 32 (sigma H) factor
RA 3,768,410 G→T L78F (TTG→TTT yibG → tetratricopeptide‑like domain‑containing protein YibG
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,138,121 C→A L305F (TTG→TTT gldA ← L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,565,464 C→A L129F (TTG→TTT yjiM ← putative dehydratase subunit
RA 4,621,303 G→A A141T (GCA→ACA)  deoD → purine nucleoside phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104081 1112 16 [15] [14] 16 mutY mutY
* * ÷ NC_000913 3423770–3424233 3424527–3424242 10–758 20 [14] [15] 16 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4035227–4035733 4036972–4035735 3–1746 16 [15] [12] 16 [rrsA] [rrsA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)102 (1.520) 65/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =117 (1.700)94 (1.370) 56/274 0.1 61.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)74 (1.070) 47/276 0.3 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA