breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 283,582 C→T S344S (TCC→TCT betT‑II → choline/carnitine/betaine transporter
RA 787,253 C→T A123V (GCC→GTC)  ettA → ADP/ATP ratio sensor/translation inhibitor
RA 963,645 C→T A84V (GCG→GTG)  phnC → phosphonate ABC transporter ATP‑binding protein
RA 1,173,697 A→G L112P (CTG→CCG)  PP_1028 ← LysR family transcriptional regulator
RA 1,201,211 C→T S134S (AGC→AGT
P5S (CCG→TCG) 
xcpU →
xcpV →
type II secretion pathway protein XcpU
type II secretion pathway protein XcpV
RA 1,261,817 (G)6→7 coding (824/1116 nt) PP_1104 ← AotO‑related protein
RA 1,348,789 A→G intergenic (‑249/+505) galP‑I ← / ← PP_1174 porin‑like protein/hypothetical protein
RA 1,485,547 (G)6→7 coding (429/1098 nt) yhdY → amino acid ABC transporter permease
RA 1,680,017 T→G T276P (ACC→CCC)  PP_1475 ← CAIB/BAIF family protein
RA 1,825,254 G→T Y14* (TAC→TAA mutS ← DNA mismatch repair protein MutS
RA 2,123,260 T→C R41R (CGT→CGC PP_1886 → hypothetical protein
RA 2,533,751 C→T S14L (TCG→TTG)  PP_2222 → hypothetical protein
RA 2,601,596 A→G T324A (ACC→GCC)  PP_2277 → hypothetical protein
RA 2,613,426 A→G T134A (ACC→GCC)  PP_2287 → internal core protein
RA 2,758,975 T→C A394A (GCA→GCG PP_2411 ← MFS transporter
RA 3,009,219 A→G M614T (ATG→ACG)  PP_2628 ← ABC transporter ATP‑binding protein
RA 3,254,711 C→A G232G (GGG→GGT PP_2851 ← hypothetical protein
RA 3,260,681 (C)6→7 intergenic (‑38/+24) PP_2855 ← / ← PP_2856 hypothetical protein/hypothetical protein
RA 3,368,556 T→C intergenic (+257/‑104) PP_2962 → / → PP_2964 alcohol dehydrogenase/transposase
RA 3,533,129 T→C L994P (CTG→CCG)  dnaEB → error‑prone DNA polymerase
RA 3,824,314 C→G A279P (GCG→CCG)  kguT ← 2‑ketogluconate transporter, putative
RA 3,824,719 T→C M144V (ATG→GTG)  kguT ← 2‑ketogluconate transporter, putative
RA 3,827,925 G→A S29F (TCC→TTC)  ptxS ← 2‑ketogluconate utilization repressor
RA 3,942,797 G→A S123S (AGC→AGT PP_3475 ← type II secretion system pseudopilin PulG‑like signal peptide protein
RA 4,056,572 (G)6→7 coding (1180/1320 nt) PP_3573 → monooxygenase
RA 4,143,477 T→C R158R (CGT→CGC PP_3645 → L‑proline amide hydrolase
RA 4,169,591 (C)8→9 intergenic (‑150/+12) creA ← / ← katG creatinase/catalase‑peroxidase
RA 4,341,656 A→G E266G (GAG→GGG)  ribAB‑II → 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 4,618,760 G→A G1055S (GGC→AGC)  PP_4085 → rhs family protein
RA 4,637,297 A→G S143P (TCT→CCT)  uvrY ← BarA/UvrY two‑componenent system response regulator
RA 4,743,195 +C intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,197 A→G intergenic (+466/+286) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,199 C→T intergenic (+468/+284) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,202 C→T intergenic (+471/+281) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,205 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,207 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,221 C→T intergenic (+490/+262) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,223 A→C intergenic (+492/+260) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,226 2 bp→GC intergenic (+495/+256) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,958,434 (C)5→6 coding (288/1374 nt) fliI ← flagellum‑specific ATP synthase
RA 5,060,944 (C)6→7 coding (279/1128 nt) ooxB ← opine oxidase subunit B
RA 5,367,931 G→A P199S (CCC→TCC)  ftsH ← integral membrane ATP‑dependent zinc metallopeptidase
RA 5,489,180 G→A G180D (GGC→GAC)  PP_4824 → two‑component system sensor histidine kinase/response regulator
RA 5,585,751 C→T A652T (GCG→ACG)  parC ← DNA topoisomerase IV subunit A
RA 5,607,194 C→T A242A (GCG→GCA PP_4926 ← HlyD family secretion protein
RA 5,641,230 (C)7→8 coding (349/1320 nt) PP_4950 → hypothetical protein
RA 5,679,880 (C)10→11 coding (99/1860 nt) PP_4983 ← amine oxidase
RA 5,749,795 T→C T53A (ACA→GCA)  PP_5043 ← PhoPQ‑activated pathogenicity‑like protein
RA 5,773,577 G→A A455V (GCC→GTC)  betT‑I ← choline transporter
RA 5,895,281 A→G intergenic (‑34/+177) PP_5167 ← / ← cysA hypothetical protein/sulfate/thiosulfate ABC transporter ATP‑binding protein
RA 6,032,315 (A)9→8 intergenic (+22/+45) PP_5729 → / ← rpmG hypothetical protein/50S ribosomal protein L33

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 27220 27542 323 8 [6] [7] 8 PP_0022/PP_0023 hypothetical protein/hypothetical protein
* * ÷ NC_002947 97558 97611 54 8 [7] [5] 9 [PP_0090]–[PP_0091] [PP_0090],[PP_0091]
* * ÷ NC_002947 170777 170867 91 8 [7] [6] 9 [PP_0159] [PP_0159]
* * ÷ NC_002947 171364–172964 176300–172968 5–4937 8 [5] [6] 9 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947 176641–178394 181784–178398 5–5144 8 [6] [7] 8 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 681258 681328 71 8 [7] [7] 9 [PP_0583] [PP_0583]
* * ÷ NC_002947 1704658 1704712 55 8 [6] [7] 8 [PP_1499] [PP_1499]
* * ÷ NC_002947 1947824 1947877 54 17 [6] [7] 8 PP_1748/PP_1749 hypothetical protein/acetyltransferase
* * ÷ NC_002947 2004043 2004349 307 8 [7] [7] 8 PP_1787 hypothetical protein
* * ÷ NC_002947 2007431 2008372 942 8 [7] [7] 8 PP_1791 aldolase/synthase
* * ÷ NC_002947 2297794 2297842 49 9 [7] [4] 8 PP_5496/PP_2023 hypothetical protein/glutathione S‑transferase family protein
* * ÷ NC_002947 2476115 2476118 4 20 [0] [0] 8 PP_2167/tal PhoD‑like phosphatase/transaldolase
* * ÷ NC_002947 3055136 3055221 86 8 [7] [7] 9 [PP_2664]–[agmR] [PP_2664],[agmR]
* * ÷ NC_002947 4172118 4172190 73 9 [5] [7] 8 katG/PP_3669 catalase‑peroxidase/LysR family transcriptional regulator
* * ÷ NC_002947 4325104 4325208 105 8 [7] [7] 8 PP_3792/PP_5631 hypothetical protein/hypothetical protein
* * ÷ NC_002947 4531961 4532022 62 11 [7] [6] 8 PP_4018/topB acetyltransferase/DNA topoisomerase III
* * ÷ NC_002947 4742766 4742844 79 8 [7] [4] 8 gltA/yeiW citrate synthase/putative oxidoreductase
* * ÷ NC_002947 4810127 4810140 14 27 [0] [0] 12 PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* * ÷ NC_002947 5023270 5023637 368 8 [5] [7] 8 [PP_4425] [PP_4425]
* * ÷ NC_002947 5099184 5099254 71 9 [7] [7] 8 PP_mr66/acsA‑I P6/acetyl‑CoA synthetase
* * ÷ NC_002947 5164459 5164592 134 9 [7] [6] 9 PP_4544/PP_4545 hypothetical protein/beta‑ketodecanoyl‑ACP synthase
* * ÷ NC_002947 5508444 5508582–5508575 132–139 9 [6] [7] 9 [PP_4839] [PP_4839]
* * ÷ NC_002947 6100261 6100460 200 9 [7] [5] 8 PP_5350 RpiR family transcriptional regulator
* * ÷ NC_002947 6162521 6162756 236 8 [6] [6] 8 PP_5740 hypothetical protein
* * ÷ NC_002947 6183852 6183858 7 36 [0] [0] NA atpI/– ATP synthase protein I/–

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 1 =0 (0.000)36 (0.680) 19/264 0.9 100% intergenic (–/+146) –/parB –/chromosome‑partitioning protein
?NC_002947 = 6183851 0 (0.000)intergenic (‑3/–) atpI/– ATP synthase protein I/–
* ? NC_002947 = 65278546 (0.870)17 (0.380) 7/224 2.1 46.6% coding (1961/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947 = 4810126 0 (0.000)intergenic (‑268/‑287) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947 = 65278646 (0.870)11 (0.210) 7/264 2.5 31.4% coding (1960/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947 = 4810125 2 (0.040)intergenic (‑267/‑288) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947 1617825 =19 (0.360)8 (0.170) 6/234 2.5 30.9% intergenic (‑38/+135) PP_1417/PP_1418 tricarboxylate transport protein TctB/tricarboxylate transport protein TctC
?NC_002947 1617914 = 19 (0.410)intergenic (‑127/+46) PP_1417/PP_1418 tricarboxylate transport protein TctB/tricarboxylate transport protein TctC
* ? NC_002947 2474067 =22 (0.420)10 (0.270) 5/186 2.3 49.4% intergenic (+6/+143) PP_2166/PP_2167 anti‑anti‑sigma factor/PhoD‑like phosphatase
?NC_002947 2474191 = 5 (0.140)intergenic (+130/+19) PP_2166/PP_2167 anti‑anti‑sigma factor/PhoD‑like phosphatase
* ? NC_002947 = 48544201 (0.020)10 (0.280) 4/182 2.5 53.1% intergenic (‑124/+173) recR/ybaB recombination protein RecR/nucleoid‑associated broad specificity regulator
?NC_002947 = 4854479 17 (0.470)intergenic (‑183/+114) recR/ybaB recombination protein RecR/nucleoid‑associated broad specificity regulator