breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 278,744 +A intergenic (‑239/+75) PP_0224 ← / ← sctC DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein
RA 307,866 +C intergenic (+881/‑988) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein
RA 336,125 +T coding (210/237 nt) PP_0278 → hypothetical protein
RA 353,067 A→G intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
RA 499,203 A→G intergenic (+47/‑249) uhpA → / → PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
RA 1,070,248 +GA intergenic (+48/+94) aroP‑I → / ← PP_0928 aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
RA 1,126,647 +C intergenic (‑101/+48) tdcG‑II ← / ← gcvP‑I L‑serine dehydratase/glycine dehydrogenase
RA 1,419,549 +C intergenic (‑38/+98) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein
RA 1,499,480 2 bp→AC intergenic (+57/‑106) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,498 1 bp→CG intergenic (+75/‑89) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,508 +C intergenic (+85/‑79) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,647,927 G→A Y265Y (TAC→TAT oprB‑II ← carbohydrate‑selective porin
RA 1,932,640 +C intergenic (‑107/+43) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
RA 2,921,460 G→A G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin
RA 2,941,574 C→T A269V (GCG→GTG)  PP_2574 → LysR family transcriptional regulator
RA 3,845,705 +G intergenic (‑72/+26) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
RA 4,586,030 2 bp→TC intergenic (+113/+101) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,035 +G intergenic (+118/+97) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,057 +C intergenic (+140/+75) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,591,702 G→A R258H (CGC→CAC)  liuC → methylglutaconyl‑CoA hydratase
RA 4,621,030 (A)10→9 coding (40/417 nt) PP_5654 → phosphotriesterase
RA 4,635,368 C→A A130S (GCC→TCC)  uvrY ← BarA/UvrY two‑componenent system response regulator
RA 4,740,811 (T)7→6 intergenic (+48/+705) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,819 2 bp→CT intergenic (+56/+696) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,229 A→C intergenic (+466/+287) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,231 C→G intergenic (+468/+285) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,234 C→T intergenic (+471/+282) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,236 2 bp→TG intergenic (+473/+279) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,239 A→G intergenic (+476/+277) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,243 3 bp→TGC intergenic (+480/+271) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,255 C→T intergenic (+492/+261) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,257 A→C intergenic (+494/+259) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,260 2 bp→GC intergenic (+497/+255) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,263 A→C intergenic (+500/+253) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,272 A→G intergenic (+509/+244) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,274 A→C intergenic (+511/+242) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,276 G→C intergenic (+513/+240) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,278 C→T intergenic (+515/+238) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,283 +T intergenic (+520/+233) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,289 C→G intergenic (+526/+227) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,292 C→T intergenic (+529/+224) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,294 C→G intergenic (+531/+222) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,296 A→C intergenic (+533/+220) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,298 C→T intergenic (+535/+218) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,964,546 C→A G271V (GGT→GTT)  fleQ ← transcriptional regulator FleQ
JC 4,980,585 +GGC intergenic (‑24/+44) PP_4387 ← / ← flgE hypothetical protein/flagellar hook protein FlgE
JC 5,555,608 +GCC intergenic (‑84/+87) PP_4887 ← / ← PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 5,674,751 +G intergenic (+113/+27) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,674,753 G→C intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
JC 5,681,415 +CGGG intergenic (‑133/+71) PP_4986 ← / ← PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
RA 6,013,885 T→C intergenic (+90/+20) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171384–172964 176274–172969 6–4891 9 [7] [7] 8 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947 176678–178394 181678–178399 6–5001 8 [7] [7] 8 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 5525463 5525603 141 8 [7] [7] 9 PP_4858 hypothetical protein
* * ÷ NC_002947 6098369 6098467 99 8 [6] [7] 8 PP_5350 RpiR family transcriptional regulator

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 49920211 (0.360)8 (0.280) 7/272 2.5 34.5% intergenic (+46/‑250) uhpA/PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
?NC_002947 = 499271 20 (0.700)intergenic (+115/‑181) uhpA/PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
* ? NC_002947 2301453 =0 (0.000)33 (1.110) 25/284 0.3 100% intergenic (‑97/‑234) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 2925429 = NA (NA)intergenic (‑177/+388) PP_2563/PP_2564 antibiotic biosynthesis protein/quercetin 2,3‑dioxygenase
* ? NC_002947 = 23014540 (0.000)24 (0.800) 23/286 0.4 100% intergenic (‑98/‑233) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 5545321 = NA (NA)intergenic (‑461/+416) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NC_002947 = 24721158 (0.260)6 (0.210) 6/276 2.9 31.9% intergenic (+19/+130) PP_2166/PP_2167 anti‑anti‑sigma factor/PhoD‑like phosphatase
?NC_002947 2472313 = 18 (0.620)coding (1783/1851 nt) PP_2167 PhoD‑like phosphatase
* ? NC_002947 = 295708015 (0.490)8 (0.270) 8/280 2.4 29.9% intergenic (+35/‑161) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
?NC_002947 2957165 = 23 (0.780)intergenic (+120/‑76) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein