breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,814 Δ1 bp 41.1% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,817 A→T 42.5% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,850 C→T 52.1% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,858 T→A 49.9% intergenic (+89/+19) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,876 C→T 55.0% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,913 C→G 15.6% S213S (TCG→TCC USA300HOU_0026 ← IS431mec transposase
RA 35,940 G→A 22.7% R204R (CGC→CGT USA300HOU_0026 ← IS431mec transposase
RA 35,970 T→C 58.9% E194E (GAA→GAG USA300HOU_0026 ← IS431mec transposase
RA 35,997 T→C 28.0% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase
RA 36,015 C→T 27.2% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,087 A→G 58.5% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase
RA 36,119 C→T 28.7% V145I (GTA→ATA)  USA300HOU_0026 ← IS431mec transposase
RA 36,124 G→A 58.0% A143V (GCT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,133 A→G 28.7% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,161 T→C 28.5% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,174 T→C 26.7% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase
RA 36,269 C→T 56.2% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase
RA 36,315 G→A 55.0% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase
RA 36,378 C→T 86.9% L58L (TTG→TTA USA300HOU_0026 ← IS431mec transposase
RA 36,413 C→T 49.0% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase
RA 36,444 C→T 47.9% R36R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,471 A→G 24.0% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase
RA 36,479 T→C 15.2% T25A (ACA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,501 G→A 6.9% A17A (GCC→GCT USA300HOU_0026 ← IS431mec transposase
RA 36,581 C→T 40.6% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 76,180 C→T 18.3% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 8.4% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 26.2% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 54.7% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 56.0% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 51.5% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,651 C→T 11.0% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 16.2% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 35.3% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 34.4% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 12.5% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 394,213 G→A 7.4% V140V (GTC→GTT USA300HOU_0361 ← hypothetical membrane protein
RA 512,643 A→G 46.4% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 728,366 G→A 6.5% G50S (GGT→AGT)  USA300HOU_0675 → hypothetical membrane protein
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,744,607 T→C 20.6% D22G (GAT→GGT)  apt ← adenine phosphoribosyltransferase
RA 1,915,579 T→C 6.1% T81T (ACA→ACG metK ← methionine adenosyltransferase
RA 1,929,796 T→C 34.4% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 36.1% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 35.8% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 37.9% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 48.1% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 57.0% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 59.5% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 56.4% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,863:1 +T 37.9% intergenic (‑946/‑222) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,864 A→T 16.2% intergenic (‑947/‑221) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 37.6% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865:1 +C 8.8% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 50.4% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 54.6% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,868:1 +T 3.7% intergenic (‑951/‑217) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 53.9% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 53.1% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 53.8% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 54.4% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 44.8% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 43.2% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 41.6% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 42.0% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 41.6% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 35.8% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 31.5% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,952,639 A→G 11.2% Y700Y (TAT→TAC epiB ← lantibiotic epidermin biosynthesis protein EpiB
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,309,899 G→T 12.4% S322* (TCA→TAA)  USA300HOU_2169 ← possible iron (Fe3+) ABC superfamily ATP binding cassette transporter, binding protein
RA 2,485,771 A→C 8.1% intergenic (‑32/+292) mqo ← / ← USA300HOU_2349 malate dehydrogenase (acceptor)/LctP family L‑lactate permease
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,557,393 A→G 30.5% D281G (GAT→GGT)  glxK → glycerate kinase
RA 2,805,347 G→T 11.6% T259T (ACC→ACA secA2 ← Sec family Type I general secretory pathway protein SecA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 49971359 (1.060)5 (0.090) 3/248 NT 8.0% coding (2417/4500 nt) gltB1 glutamate synthase (NADPH), large subunit
?CP000730 = 499723 58 (1.090)coding (2427/4500 nt) gltB1 glutamate synthase (NADPH), large subunit
* ? CP000730 512620 =63 (1.140)48 (1.260)
+40 bp
30/178 NT 52.6% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 1442900 =NA (NA)4 (0.080) 3/246 NT NA intergenic (+133/+135) telA/USA300HOU_1341 tellurite resistance protein/IS1272 transposase
?CP000730 1442915 = NA (NA)intergenic (+148/+120) telA/USA300HOU_1341 tellurite resistance protein/IS1272 transposase
* ? CP000730 1766474 =48 (0.870)4 (0.080) 3/240 NT 8.9% coding (379/561 nt) tag 3‑methyladenine glycosidase
?CP000730 1766523 = 37 (0.720)coding (428/561 nt) tag 3‑methyladenine glycosidase