breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 39,672 C→A 100% V488F (GTT→TTT)  mecA ← penicillin binding protein 2 prime
RA 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,660 T→A 17.3% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 37.4% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 35.6% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 459,019 G→A 9.4% G27E (GGG→GAG)  hsdS1 → type I site‑specific deoxyribonuclease specificity subunit
RA 512,643 A→G 41.3% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 607,884 G→A 100% R209* (CGA→TGA)  USA300HOU_0550 ← deoxynucleoside kinase
RA 629,681 C→A 100% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 100% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase
RA 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 745,728 A→G 16.8% K9E (AAA→GAA)  USA300HOU_0690 → hypothetical protein
RA 804,673 A→T 100% L135I (TTA→ATA)  USA300HOU_0751 ← POT family proton (H+)‑dependent oligopeptide transporter
RA 933,033 T→C 100% intergenic (‑74/‑27) USA300HOU_0897 ← / → USA300HOU_0898 possible NifU family protein/hypothetical protein
RA 1,064,445 Δ1 bp 100% coding (44/1218 nt) USA300HOU_1000 ← LytR family transcriptional regulator
RA 1,233,504 T→A 100% V470D (GTT→GAT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,430,220 T→C 10.5% intergenic (+329/‑531) USA300HOU_1327 → / → lysC2 ABC superfamily ATP binding cassette transporter binding protein/aspartate kinase
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
MC JC 1,747,382 (TAC)3→2 100% coding (2065‑2067/2280 nt) secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,028,583 G→T 100% A21D (GCC→GAC)  USA300HOU_1882 ← hypothetical membrane protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,257 A→C 66.7% V78G (GTA→GGA)  USA300HOU_2153 ← hypothetical membrane protein
RA 2,352,815 G→A 100% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,480,567 C→T 8.4% R113H (CGC→CAC)  htrA ← hemin ABC superfamily ATP binding cassette transporter, membrane protein
MC JC 2,501,188 Δ3,014 bp 100% USA300HOU_2364 USA300HOU_2364
RA 2,530,133 T→A 11.5% intergenic (‑196/+99) USA300HOU_2386 ← / ← USA300HOU_2387 ABC superfamily ATP binding cassette transporter, binding protein/hypothetical protein
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,590,608 G→T 10.0% L84L (CTG→CTT USA300HOU_2452 → hypothetical protein
RA 2,709,609 C→A 18.6% E440* (GAG→TAG)  USA300HOU_2562 ← possible acetyltransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =49 (1.060)26 (0.820)
+40 bp
19/178 NT 43.6% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 2660570 =43 (0.930)4 (0.100) 4/234 NT 9.9% intergenic (+12/+237) ddh/USA300HOU_2514 D‑lactate dehydrogenase/HAD superfamily hydrolase
?CP000730 2660603 = 34 (0.810)intergenic (+45/+204) ddh/USA300HOU_2514 D‑lactate dehydrogenase/HAD superfamily hydrolase