breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 367,644 G→T intergenic (‑96/‑20) nanA ← / → nagC N‑acetylneuraminate lyase/possible transcriptional regulator
RA 479,553 G→A A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein
RA 622,314 C→A S959R (AGC→AGA sdrE → Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrE
RA 632,405 T→G I177S (ATT→AGT)  proP → MFS family major facilitator transporter, proline/betaine:cation symporter
RA 638,614 G→T T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase
RA 707,879 A→T L11I (TTA→ATA)  mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 790,778 G→A intergenic (+277/‑197) kipA → / → ltaS possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
RA 1,006,124 A→T S169R (AGT→AGA yjbH ← possible dithiol‑disulfide isomerase
RA 1,058,293 G→A A1215V (GCA→GTA)  atl ← bifunctional N‑acetylmuramoyl‑L‑alanine amidase/mannosyl‑glycoprotein endo‑beta‑N‑acetylglucosaminidase
RA 1,232,465 G→C A124P (GCG→CCG)  prkC → non‑specific serine/threonine protein kinase
RA 1,329,645 C→T P281L (CCA→CTA)  glpD → glycerol‑3‑phosphate dehydrogenase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,007 Δ1 bp coding (34/201 nt) cspA ← cold shock protein CspA
RA 1,474,639 C→G A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein
RA 1,481,355 G→T A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,454 C→T G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase
RA 1,797,772 G→C R7G (CGC→GGC)  phoR ← sensor histidine kinase
RA 1,834,119 G→A intergenic (+68/‑46) ymaD → / → sgaA hypothetical protein/class V aminotransferase
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,143,110 G→A V163V (GTG→GTA USA300HOU_2026 → hypothetical membrane protein
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,856,087 T→A P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =176 (1.020)133 (1.060)
+40 bp
56/216 0.4 50.9% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA