Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A110 F1 I1 R1 14 278.2 17968888 92.5% 16621221 75.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,193,7790GT99.5% 712.1 / NA 204L143I (CTT→ATT) yeiBconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (72/130);  total (73/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑GCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851
                                                                              |                                                                        
gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑Tcc                                                                        <  1:4183034/76‑1 (MQ=255)
gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑Tcc                                                                        <  1:1662666/76‑1 (MQ=255)
gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑Tcc                                                                        <  1:5288631/76‑1 (MQ=255)
  cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGT‑C‑GG‑GAAACAGCCCCCATAAAAGCAAAACA‑CCCA‑Acccc                                                                      >  2:6233601/1‑76 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGTTTCGCCGT‑C‑GGAAAAAAAGCCCCAATAACACAAAAACC‑CCAT‑Acccc                                                                      >  2:6875916/1‑76 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGACGT‑C‑GG‑GAATCAGCCCCAACAACAGCAAAAAG‑CCAA‑Cccccc                                                                     >  1:8901680/1‑75 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGG‑C‑GG‑GAAACAACCCCCAAAACAACAAAACG‑CCAA‑AACcca                                                                     >  1:8170076/1‑76 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAAAAAGCCCCCAAAACAGCCAAAAG‑CCCA‑TCCcca                                                                     >  1:5340201/1‑76 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAAAAAGACCCCAAAAAAACAAAAAG‑CCAA‑Accccc                                                                     >  1:6957906/1‑75 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑CGGG‑AAAACACCCCCCCAACCGGGAAAACA‑CCAA‑Acccc                                                                      >  2:3878474/1‑76 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑C‑GG‑GAAACAACCCCCAAAACAACAAAAAC‑CCAA‑Accccc                                                                     >  1:7706129/1‑75 (MQ=255)
    aTGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAAACAGACCCCAAAAAAGCAAAAAG‑CCAA‑Tcccccc                                                                    >  1:3651407/1‑74 (MQ=255)
     tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGTGG‑GG‑AAATAAGCCCCAAAAAAAAC‑AAACG‑CCAA‑AACccacc                                                                   >  2:7778396/1‑76 (MQ=255)
      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCccacca                                                                  <  2:6927620/76‑1 (MQ=255)
      gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAAACAGCCCCCATAACAGCAAAAAG‑CCAA‑AACccacca                                                                  >  1:6923638/1‑76 (MQ=255)
       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAg                                                                 <  2:819273/76‑1 (MQ=255)
       cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑CAAAAAGCCCCCATAAAAACAAAACGCCCAA‑ACCccacac                                                                  >  1:3222529/1‑74 (MQ=255)
       cATCCGGCGTCCAGGCGCGGCTGGGCTGGCCGG‑C‑CG‑GAAAAAGCCCCAAAAAAAAGAAAACG‑GCCA‑GACccaccca                                                                 >  2:1014769/1‑74 (MQ=255)
         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGAt                                                               <  1:2084069/76‑1 (MQ=255)
          ccGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAAACAACCCCCATAAACGCAAAAAA‑CCCA‑ATCCCACCCGATa                                                              >  2:5023868/1‑76 (MQ=255)
           cGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATaa                                                             <  1:5045558/76‑1 (MQ=255)
           cGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAATCAGCCCCAAAAAAAGCAAAACG‑CCCA‑TCCCCACCCGCTaa                                                             >  1:6547357/1‑76 (MQ=255)
           cGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAAACAGCCCCAAAAACAACAAAACG‑CCAA‑CCCCCCCCCGACaa                                                             >  1:7765297/1‑76 (MQ=255)
            ggCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATaaa                                                            <  1:8624676/76‑1 (MQ=255)
              cGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGc                                                          <  2:3794303/76‑1 (MQ=255)
              cGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GG‑GAATCAGCCCCCATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGc                                                          >  1:2729180/1‑76 (MQ=255)
               gTCCAGGCGCGGCTGGTCTGTCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCCCCAGATAAAGCa                                                         <  2:4944920/76‑1 (MQ=255)
               gTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCa                                                         <  2:2657493/76‑1 (MQ=255)
                tCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCAt                                                        <  1:4549785/76‑1 (MQ=255)
                 ccAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATg                                                       <  1:2392042/76‑1 (MQ=255)
                 ccAGGCGCGGCTGGGCTGGCTGT‑T‑GG‑GAATCAAGCCCCATAAAAGCCAAACG‑CCAATCCCCCACCA‑AAAAAGCATg                                                       >  2:4146743/1‑76 (MQ=255)
                  cAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGa                                                      <  1:6090048/76‑1 (MQ=255)
                   aGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGAc                                                     <  1:110853/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTg                                                <  1:5159645/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTg                                                <  1:3547779/76‑1 (MQ=255)
                           cTGGTCTGGCTGT‑C‑GG‑ACATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:8458927/76‑1 (MQ=255)
                            tGGTCTGGCTGT‑C‑GG‑AACTCAGCCCCAATTACAGCAAAACG‑GCAA‑TCCCCACCAGATAAAGCATGACGCCTGCAtt                                            >  2:1031062/1‑76 (MQ=255)
                            tGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:979336/76‑1 (MQ=255)
                             ggTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:4539511/76‑1 (MQ=255)
                             ggTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  1:1850377/1‑76 (MQ=255)
                             ggTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  2:7315564/76‑1 (MQ=255)
                              gTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:8010516/76‑1 (MQ=255)
                              gTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  2:6124567/1‑76 (MQ=255)
                               tCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  2:3339965/1‑76 (MQ=255)
                               tCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  2:7964643/76‑1 (MQ=255)
                                cTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTg                                                >  2:7407713/1‑68 (MQ=255)
                                cTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTg                                                <  1:34004/68‑1 (MQ=255)
                                cTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTg                                                >  2:5465935/1‑68 (MQ=255)
                                cTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:1534887/76‑1 (MQ=255)
                                 tGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:743008/1‑76 (MQ=255)
                                 tGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:3931180/1‑76 (MQ=255)
                                 tGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  2:6856561/1‑76 (MQ=255)
                                 tGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:1505083/1‑76 (MQ=255)
                                  ggCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAACCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  2:1733012/1‑76 (MQ=255)
                                  ggCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  2:5955868/76‑1 (MQ=255)
                                  ggCTGT‑C‑GG‑AAAGCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  1:2044705/76‑1 (MQ=255)
                                    cTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  1:4344385/76‑1 (MQ=255)
                                     tGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:4775988/1‑76 (MQ=255)
                                     tGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:452430/1‑76 (MQ=255)
                                     tGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  1:1289840/1‑76 (MQ=255)
                                     tGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:2365494/1‑76 (MQ=255)
                                      gT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  1:2007089/1‑76 (MQ=255)
                                      gT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:6504390/76‑1 (MQ=255)
                                       t‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 >  2:2315279/1‑76 (MQ=255)
                                       t‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:1930862/76‑1 (MQ=255)
                                       t‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:2623136/76‑1 (MQ=255)
                                         c‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  1:4659688/1‑76 (MQ=255)
                                           gg‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:7122873/76‑1 (MQ=255)
                                           gg‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:4586622/76‑1 (MQ=255)
                                           gg‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:4801984/76‑1 (MQ=255)
                                           gg‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:4853535/76‑1 (MQ=255)
                                            g‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              >  2:583584/1‑76 (MQ=255)
                                              aaaTCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  2:6348822/76‑1 (MQ=255)
                                              aaaTCAGCCCCAATAACAGCAAAACG‑CCAA‑GCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  1:3365469/76‑1 (MQ=255)
                                                aTCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:7027082/76‑1 (MQ=255)
                                                aTCAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:8431512/76‑1 (MQ=255)
                                                  cAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:5154426/76‑1 (MQ=255)
                                                  cAGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:5201010/76‑1 (MQ=255)
                                                   aGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:8732530/76‑1 (MQ=255)
                                                   aGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  1:2783184/76‑1 (MQ=255)
                                                   aGCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        >  1:4507389/1‑76 (MQ=255)
                                                    gCCCCAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       >  2:3037196/1‑76 (MQ=255)
                                                      cccAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAATAGGCTTTTTACCGATGgcg                     >  1:7167346/1‑76 (MQ=255)
                                                       ccAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  1:4135113/1‑76 (MQ=255)
                                                       ccAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  2:3243139/1‑76 (MQ=255)
                                                        cAATAACAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACTCCTGTATTACACAGGCTTTGTACCGATGGCGCa                   >  2:7648765/1‑76 (MQ=255)
                                                            aaCAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  2:8096570/1‑76 (MQ=255)
                                                              cAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:3453902/76‑1 (MQ=255)
                                                              cAGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:4082959/76‑1 (MQ=255)
                                                               aGCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            <  1:2115125/76‑1 (MQ=255)
                                                                gCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:443020/76‑1 (MQ=255)
                                                                gCAAAACG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:2833617/76‑1 (MQ=255)
                                                                  aaaaCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCTCCa         <  1:5795141/76‑1 (MQ=255)
                                                                  aaaaCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:6531794/1‑76 (MQ=255)
                                                                  aaaaCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:7838859/1‑76 (MQ=255)
                                                                   aaaCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:1255522/76‑1 (MQ=255)
                                                                    aaCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:201143/1‑76 (MQ=255)
                                                                    aaCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       <  1:4854987/76‑1 (MQ=255)
                                                                    aaCG‑CCAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       <  2:7711505/76‑1 (MQ=255)
                                                                         ccAA‑TCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  1:5539814/1‑76 (MQ=255)
                                                                              |                                                                        
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GG‑AAATCAGCCCCAATAACAGCAAAACG‑CCAA‑GCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A