Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
14 |
24.7 |
1576304 |
96.5% |
1521133 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,193,779 |
G→T |
L143I (CTT→ATT) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,193,779 | 0 | G | T | 95.2%
| 57.0
/ NA
| 21 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (5/15); minor base C (1/0); total (6/15) |
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACC > REL606/2193707‑2193843
|
ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc > 2:578259/1‑76 (MQ=255)
cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc > 2:256296/1‑76 (MQ=255)
ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 1:441153/76‑1 (MQ=255)
gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa > 1:172479/1‑76 (MQ=255)
cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:193593/76‑1 (MQ=255)
cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa > 2:268802/1‑76 (MQ=255)
aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 2:226270/76‑1 (MQ=255)
ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 2:427316/76‑1 (MQ=255)
gcgcGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc < 1:751840/76‑1 (MQ=255)
gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAGTCCCCACCAGATAAAGCATGACGCCt < 2:95819/76‑1 (MQ=255)
gacTGGTCTGGCTGTCGGAAAACAGCCCCGATGACAGCAATACGCCAAACCCCACNAGATAAAGCATAACGCCTGt < 1:92358/74‑1 (MQ=255)
tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:268769/76‑1 (MQ=255)
tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 1:31796/76‑1 (MQ=255)
tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAACCCCCACCAGATAAAGCATGACGCCTGTATTaaa > 1:623442/1‑76 (MQ=255)
ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 2:172479/76‑1 (MQ=255)
gAAATCAGCCCCATTAACAGCAAAACGTCAATCCCCACCAGGTAAAGCATGACGCCTGTATTAAACACCCTTTTTa < 2:701343/76‑1 (MQ=255)
gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATTNAGCATGACGTCTGTATTAAACAGGCTTTTTa < 1:71408/76‑1 (MQ=255)
gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:177078/76‑1 (MQ=255)
aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCATATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:551078/76‑1 (MQ=255)
ccccAATAACAGCAAAACGCCAATCCCCACCAGTTAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 1:625951/76‑1 (MQ=255)
cacAATCACAGCAAAACGCCAANCCTCACCAGANAAAGCATGNCGCCTGTATTAAACAGGCGTTTNACCGTTGgcg < 2:682185/74‑1 (MQ=255)
tAACAGCAAAACGGCAATCCTCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg < 2:660687/76‑1 (MQ=255)
cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:169667/1‑76 (MQ=255)
|
CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACC > REL606/2193707‑2193843
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
