Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
17 |
37.0 |
2377229 |
96.2% |
2286894 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,193,779 |
G→T |
L143I (CTT→ATT) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,193,779 | 0 | G | T | 100.0%
| 73.6
/ NA
| 28 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (10/18); total (10/18) |
TGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC > REL606/2193711‑2193850
|
tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 2:293945/76‑1 (MQ=255)
tGCATCCGGCGTCCAGGCGCGGCTGGACCGGCTGTCGGAAATCAGCCCCAATAACAGCAACAAGCCAATCCccacc > 2:896599/1‑76 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 1:451546/76‑1 (MQ=255)
gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:588507/76‑1 (MQ=255)
gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:416074/76‑1 (MQ=255)
cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCGACCAGATAAAGCATGACGCCTg > 1:858834/1‑76 (MQ=255)
cTGGTCTGGCTGTCGGATAGCAGCNCCAATAACAGCAAAACGCCAATCCCCACCAAATNAAGCATGACGCCTGTAt < 2:991002/76‑1 (MQ=255)
cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:329911/76‑1 (MQ=255)
cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:221797/76‑1 (MQ=255)
tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCACACCAGATAAAGCATGACGCCTGTAtt > 1:707377/1‑76 (MQ=255)
ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 2:601309/1‑76 (MQ=255)
ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 2:168036/76‑1 (MQ=255)
ggTCTGACTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 1:175179/1‑76 (MQ=255)
gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:35562/76‑1 (MQ=255)
tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:140909/76‑1 (MQ=255)
ggCTGTCGGCAATCAGCNCCCATAACAGCAAAACGCCAATCCCCACCAGATNAAGCATCACCCCTGTATTATACAg > 2:993881/1‑76 (MQ=255)
ggCTGTCGGAAATCAGCCCCAATAACAGGAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 2:539782/76‑1 (MQ=255)
cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc < 1:626582/76‑1 (MQ=255)
gAAATCAGCCCCAATAACNGCAAAACGCCAATCGCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:939691/76‑1 (MQ=255)
aaaTCAGCCCCAATAACAGCGAAACGCCAATCCCCACCAGATAAAGCATGACTCCTGTATTAAACAGGCTTTTTAc < 1:896599/76‑1 (MQ=255)
aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg < 2:114286/76‑1 (MQ=255)
ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc > 2:938766/1‑76 (MQ=255)
aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCATGTATTAAACAGGCTTTTTACCGATGGAGCATcgc > 1:379218/1‑76 (MQ=255)
aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 2:324767/76‑1 (MQ=255)
cgaaaCGCCAATCCCCACCAGAAAAAGCACGACGCCTGTATTAAACAGGCTTCTTACCGATGGCGCATCTCGCAcc > 1:1027701/3‑76 (MQ=255)
aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:812917/76‑1 (MQ=255)
aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg > 2:892738/1‑76 (MQ=255)
aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:403552/76‑1 (MQ=255)
cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 2:723547/1‑76 (MQ=255)
ccAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc < 2:868788/76‑1 (MQ=255)
|
TGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC > REL606/2193711‑2193850
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
