Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A137 F1 I1 R1 18 221.5 13936938 95.6% 13323712 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,193,7790GT99.5% 717.6 / NA 181L143I (CTT→ATT) yeiBconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (67/113);  minor base A (1/0);  total (68/113)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851
                                                                          |                                                                        
gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATcc                                                                        <  2:6670565/76‑1 (MQ=255)
 ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATccc                                                                       <  2:3350401/76‑1 (MQ=255)
 ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGGCGGGAAACAGCCCCCAAAACACGCAAAA‑GCCAAAccc                                                                       >  1:1831573/1‑76 (MQ=255)
  cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTGGGGAAATAGCCCCCATAAAC‑GCAAAACCCCCAAcccc                                                                      >  1:859530/1‑76 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCcca                                                                     <  1:265197/76‑1 (MQ=255)
    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCccac                                                                    <  1:3054836/76‑1 (MQ=255)
    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCccac                                                                    <  2:6817718/76‑1 (MQ=255)
    aTGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCCGGAAACAACCCCCAAAACA‑ACAAAACGCCAATcccccc                                                                    >  2:2878692/1‑74 (MQ=255)
      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAAACAGCCCCCATAACA‑GCAAAAAGCCAAACCccacca                                                                  >  1:1321061/1‑76 (MQ=255)
      gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAATCAACCCCAATAACA‑AACAAACACCAAACCccacca                                                                  >  2:150954/1‑76 (MQ=255)
        aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAGCCCCAATAACA‑GCAAAAACCCAAACCCCACCAGa                                                                >  1:1073398/1‑76 (MQ=255)
        aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCAATAAAA‑GCCAAACGCCAAACACCACCAGa                                                                >  1:504023/1‑76 (MQ=255)
        aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAAAAACCCCCATAAAA‑GCAAAACGCCCAACcccccccgc                                                                >  2:600211/1‑73 (MQ=255)
         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGAt                                                               <  2:2696075/76‑1 (MQ=255)
         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGAt                                                               <  1:3764626/76‑1 (MQ=255)
          ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATa                                                              <  2:6558312/76‑1 (MQ=255)
          ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATa                                                              <  1:1514302/76‑1 (MQ=255)
          ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATa                                                              <  1:3230235/76‑1 (MQ=255)
          ccGGCGTCCAGGCGCGGCTGGGCTGGCTGTTGGGAAACAACCCCAATAACA‑GCAAAAAGCCCATCCCCCCCCGATa                                                              >  2:4954602/1‑76 (MQ=255)
          ccGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAATCAGCCCCCATAACA‑GCAAAACGCCAAACCCCCCCAGATa                                                              >  1:3296833/1‑76 (MQ=255)
           cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaa                                                             <  1:3601420/76‑1 (MQ=255)
            ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:6070942/76‑1 (MQ=255)
            ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:4360161/76‑1 (MQ=255)
              cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  2:6624973/76‑1 (MQ=255)
              cGTCCAGGCGCGGCTGGGCTGGGTGGCGGGAAAAAGCCCCCATAACA‑GCCAAACCCCAAACCCCCCCAGATaaaaa                                                          >  2:2601862/1‑74 (MQ=255)
              cGTCCAGGCGCGGCTGGGCTGGCTGCCCGGAAACAGCGCCAATAACA‑ACAAAAAGCCACACCCCACCAGGTaaaag                                                          >  2:5825957/1‑74 (MQ=255)
               gTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCAAAAAAA‑ACCAAAAGCCAAACCCCCCCCAATaaaaac                                                         >  2:2145594/1‑73 (MQ=255)
               gTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAAACGCCCCCAAAACA‑GCAAAAAGCCAAACCCCACCCAATAAAACa                                                         >  2:6084052/1‑76 (MQ=255)
               gTCCAGGCGCGGCTGGGCTGGCTGTCGGAAAACAGCCCCCATAACA‑GCAAAAAGCCCATCCCCACCAGAaaaaaaa                                                         >  2:1717969/1‑73 (MQ=255)
                tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  2:5487069/76‑1 (MQ=255)
                tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  2:6332210/76‑1 (MQ=255)
                tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  1:3939726/76‑1 (MQ=255)
                tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  1:6642724/76‑1 (MQ=255)
                  cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                      <  1:2828118/76‑1 (MQ=255)
                  cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                      <  2:2272659/76‑1 (MQ=255)
                       gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  1:6128830/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:2954768/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:3654027/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:5547435/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:1000614/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:769289/76‑1 (MQ=255)
                         ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  1:117091/76‑1 (MQ=255)
                         ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  1:6152344/76‑1 (MQ=255)
                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  2:6209245/1‑76 (MQ=255)
                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  2:5359756/76‑1 (MQ=255)
                           cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  2:5749220/76‑1 (MQ=255)
                           cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  2:1962537/1‑76 (MQ=255)
                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:781645/1‑76 (MQ=255)
                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:6888824/1‑76 (MQ=255)
                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:4275011/1‑76 (MQ=255)
                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:1939053/76‑1 (MQ=255)
                              gTCTNGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  2:6138291/1‑76 (MQ=255)
                              gTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  1:1341300/1‑76 (MQ=255)
                                cTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:1279398/1‑76 (MQ=255)
                                 tGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  2:1662373/76‑1 (MQ=255)
                                 tGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  2:5918637/1‑76 (MQ=255)
                                      gTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  1:6191327/1‑76 (MQ=255)
                                       tCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  1:3138810/76‑1 (MQ=255)
                                        cGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:2497959/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:649661/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:5632767/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:1772210/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               >  2:393693/1‑76 (MQ=255)
                                          gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:5254429/76‑1 (MQ=255)
                                          gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:835423/76‑1 (MQ=255)
                                          gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:423447/76‑1 (MQ=255)
                                           aaaTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  1:2552120/76‑1 (MQ=255)
                                            aaTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  2:6367653/1‑76 (MQ=255)
                                             aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:2412260/76‑1 (MQ=255)
                                               cAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:3246781/76‑1 (MQ=255)
                                                aGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        >  1:5565251/1‑76 (MQ=255)
                                                 gCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       >  2:5663696/1‑76 (MQ=255)
                                                  ccccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  1:4107237/76‑1 (MQ=255)
                                                   cccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     <  1:5156022/76‑1 (MQ=255)
                                                   cccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     <  2:4613/76‑1 (MQ=255)
                                                       aTAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTACACAGGCTTTTTACCGATGGCGCATc                 >  1:3356159/1‑76 (MQ=255)
                                                        tAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  1:6240840/1‑76 (MQ=255)
                                                         aaCA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:2378368/1‑76 (MQ=255)
                                                         aaCA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:1410915/1‑76 (MQ=255)
                                                           cA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  1:2091824/1‑76 (MQ=255)
                                                            a‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            <  1:6576715/76‑1 (MQ=255)
                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          >  2:5876251/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:1672848/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:1138531/76‑1 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:1546914/76‑1 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:5900271/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:3575111/76‑1 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:5335276/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:2865794/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:3193596/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:4805767/1‑76 (MQ=255)
                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcccg        >  1:4606091/1‑74 (MQ=255)
                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:3028973/76‑1 (MQ=255)
                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       <  1:429302/76‑1 (MQ=255)
                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:1652618/1‑76 (MQ=255)
                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       <  2:4163938/76‑1 (MQ=255)
                                                                   accccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:3774495/73‑1 (MQ=255)
                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:6659227/1‑76 (MQ=255)
                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  1:6454942/1‑76 (MQ=255)
                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:1903593/1‑76 (MQ=255)
                                                                          |                                                                        
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A