Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
17 |
21.6 |
1356596 |
98.4% |
1334890 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,193,779 |
G→T |
L143I (CTT→ATT) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,193,779 | 0 | G | T | 100.0%
| 55.2
/ NA
| 21 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (8/13); total (8/13) |
GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCG > REL606/2193709‑2193848
|
gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCTcca < 1:31669/76‑1 (MQ=255)
gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca > 2:654451/1‑76 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACGAGAt < 2:461644/76‑1 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 2:391686/76‑1 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 1:363500/76‑1 (MQ=255)
cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa > 2:510935/1‑76 (MQ=255)
ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg > 1:310015/1‑76 (MQ=255)
aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 1:174851/76‑1 (MQ=255)
aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 1:119741/76‑1 (MQ=255)
tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:245775/76‑1 (MQ=255)
ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 2:255391/76‑1 (MQ=255)
tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 2:515491/76‑1 (MQ=255)
gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:656796/76‑1 (MQ=255)
aTCAGCCCCAATAACAGCAAAACGCCTATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:19007/76‑1 (MQ=255)
aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 1:131962/1‑76 (MQ=255)
aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 2:203529/1‑76 (MQ=255)
tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa < 1:542998/76‑1 (MQ=255)
cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt > 1:126569/1‑76 (MQ=255)
ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc > 1:96646/1‑76 (MQ=255)
aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 1:221395/76‑1 (MQ=255)
aaaaCGCCAATCCCCACCATATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 1:619414/76‑1 (MQ=255)
cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 1:357393/1‑76 (MQ=255)
cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGATTTTTACCGATGGCGCATCGCGCACCAgccg > 2:451965/1‑76 (MQ=255)
|
GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCG > REL606/2193709‑2193848
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
