Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
20 |
51.0 |
3438118 |
91.8% |
3156192 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,193,779 |
G→T |
L143I (CTT→ATT) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,193,779 | 0 | G | T | 100.0%
| 120.1
/ NA
| 40 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/18); total (22/18) |
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC > REL606/2193706‑2193850
|
gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc < 1:817305/76‑1 (MQ=255)
cGATGCATCCGACGTCCAGGCGCGGCTGGTCTGGCTGNCGGAAATCAGCCCCAATAACAGCAAAAAGCCAATcccc > 1:1669201/1‑76 (MQ=255)
cGANGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc > 1:1546647/1‑76 (MQ=255)
gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca < 1:1125879/76‑1 (MQ=255)
gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca > 1:852591/1‑76 (MQ=255)
gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGAAATCAGCCCCAATAACAACAAAACGCCAACCCcca > 1:375608/1‑76 (MQ=255)
tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 1:121566/76‑1 (MQ=255)
gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCACTAACAGCAAAACGCCAATCCccacca > 1:939662/1‑76 (MQ=255)
gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGGAAAACGCCAATCCccacca > 1:1251761/1‑76 (MQ=255)
gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca > 1:1442827/1‑76 (MQ=255)
gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca > 1:1558303/1‑76 (MQ=255)
gCATCCGGCGACCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca > 1:1613225/1‑76 (MQ=255)
aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa < 2:858075/76‑1 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCCGAt > 2:852765/1‑76 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAAAAGGAAAACGCCAATCCCCACCAGAt > 2:313592/1‑76 (MQ=255)
gCGTCCAGGCGCGGATGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCTATACCCTCCAGATAAAg < 1:134587/76‑1 (MQ=255)
cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCGGCCACAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGc < 1:48376/76‑1 (MQ=255)
gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa > 2:1293980/1‑76 (MQ=255)
gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa > 1:592888/1‑76 (MQ=255)
cgaGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:556148/74‑1 (MQ=255)
ccAGGCGCGGCTGGTCCGGCTGTCGGAAATCAGCCCCAAGAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:40678/76‑1 (MQ=255)
ccAGGCGCGGCTGGGCTGGCTGTCGGAAATCAGCGCCAATANCCGCAAAACGCCAATCCCNACNCGATAAAGCATg > 1:1404766/1‑76 (MQ=255)
cAGNCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa > 1:1544064/1‑76 (MQ=255)
aGGCGCGGCTGGTCTGGCTGTCGGGAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc > 2:455525/1‑76 (MQ=255)
aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 1:1292740/76‑1 (MQ=255)
tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 2:427353/76‑1 (MQ=255)
tGGTCGGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCACGACGCCTGTAtt < 2:59107/76‑1 (MQ=255)
ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 2:480978/76‑1 (MQ=255)
gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa > 1:1377058/1‑76 (MQ=255)
cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:546009/1‑76 (MQ=255)
tGGCTGTCCGAAATCAGCCCCGATAACAGCAAAACGCCAATCCCCCGCAGATAAAGCATGATGCCTGTATTAAACa > 2:24259/1‑76 (MQ=255)
ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg > 2:324072/1‑76 (MQ=255)
tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt > 2:422339/1‑76 (MQ=255)
cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 1:1553907/1‑76 (MQ=255)
ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:1477818/76‑1 (MQ=255)
aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 1:755183/1‑76 (MQ=255)
aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 1:585269/1‑76 (MQ=255)
aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc < 1:561490/76‑1 (MQ=255)
aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc < 1:1107820/76‑1 (MQ=255)
aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGTTAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:422340/76‑1 (MQ=255)
ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 1:815941/76‑1 (MQ=255)
tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg > 2:847687/1‑76 (MQ=255)
cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGTGCAGCGCGCAcc > 2:292808/1‑76 (MQ=255)
aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 1:1453944/76‑1 (MQ=255)
aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc > 2:737883/1‑76 (MQ=255)
ccNATCCCCACCAGATAAAGCATGACGCCTGTATTAAACGGGCTTTTTACCGATGGCGCATCGCGCAGCAgctncc < 2:1400400/76‑1 (MQ=255)
ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:325932/1‑76 (MQ=255)
|
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC > REL606/2193706‑2193850
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
