Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A112 F1 I1 R1
|
17 |
249.1 |
16570612 |
93.0% |
15410669 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,193,779 |
G→T |
L143I (CTT→ATT) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,193,779 | 0 | G | T | 99.4%
| 637.5
/ NA
| 169 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (53/114); total (53/116) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 185 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851
|
aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCC‑GGAAACAAACCCAATAAAAGCAAAACGCCCATcccccc > 2:6880560/1‑74 (MQ=255)
tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 1:6350169/76‑1 (MQ=255)
gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCG‑GGAAACAACCCCCATAAAACCAAAACGCCAAACCccacca > 1:2196911/1‑76 (MQ=255)
cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 1:5235043/76‑1 (MQ=255)
aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa < 1:4104387/76‑1 (MQ=255)
aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCG‑GAAATCAGCCCCCATAATAGCAAAACGACAAACCCCCACAGa > 1:855107/1‑76 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCG‑GGAATCAGCCCCAATAACAGCAAAAAGCCCAACCCCCCCAGaa > 1:489615/1‑75 (MQ=255)
ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 1:4507951/76‑1 (MQ=255)
cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 1:7968055/76‑1 (MQ=255)
cGGCGTCCAGGCGCGGCTGGTCTGGCTGGCG‑GAAATCCGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 2:8278792/76‑1 (MQ=255)
ggCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 1:1164627/76‑1 (MQ=255)
ggCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 2:1566924/76‑1 (MQ=255)
ggCGTCCAGGCGCGGCTGGGCTGGGTTTCGCGAAACCA‑CCCCAATAACAAACAAACACCCAACCCCACCCCATaaa > 2:1661844/1‑76 (MQ=255)
ggCGTCCAGGCGCGGCTGGGCTGGGCGTGG‑GGAAAAAACCCCCAAAACAGCAAAACACCAATCCCCCCCCGAaaaa > 2:6491266/1‑76 (MQ=255)
gCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:5580542/76‑1 (MQ=255)
gCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:6619311/76‑1 (MQ=255)
gCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:2075476/76‑1 (MQ=255)
cGTCCAGGCGCGGCTGGGCTGGCTGTCG‑GGAAACAGCCCCAATAAAAGCCAAACGCCCAATCCCCCCCGATAAAGc > 1:5467741/1‑76 (MQ=255)
gTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 2:4636940/76‑1 (MQ=255)
gTCCAGGCGCGGCTGGGCTGGCTGTTG‑GAAAACAACCCCAATAACAGCAAAAAACCAAATCCCACCACATAAAACa > 2:7023067/1‑76 (MQ=255)
ccAGGCGCGGCTGGGCTGGGTGTCG‑GAAAATAGCCCCCATAAAAGCAAAACACCAATCCCCACCCGAAAAAACATg > 2:6043154/1‑76 (MQ=255)
cAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:6229406/76‑1 (MQ=255)
cAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:4363936/76‑1 (MQ=255)
aGGCGCGGCTGGGCTGGCTGGCG‑GAAAACAGCCCCCAAAAAAGCAAAAAGCCCAACCCCCCCCGATAAAACATGAc > 2:3853594/1‑76 (MQ=255)
gcgGGTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 2:7518362/76‑1 (MQ=255)
gcgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:4424714/76‑1 (MQ=255)
gcgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:7031581/76‑1 (MQ=255)
cgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:2898250/76‑1 (MQ=255)
cgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:328285/76‑1 (MQ=255)
ggCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt > 1:1215576/1‑76 (MQ=255)
ggCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 1:1613468/76‑1 (MQ=255)
gCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 1:7007511/76‑1 (MQ=255)
gCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa > 1:7058866/1‑76 (MQ=255)
gCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 2:1999603/76‑1 (MQ=255)
cTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACCGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGAAt > 2:6184953/1‑76 (MQ=255)
cTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTatat > 2:4041302/1‑76 (MQ=255)
cTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:832575/76‑1 (MQ=255)
tGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:7271186/76‑1 (MQ=255)
tGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 2:7826567/1‑76 (MQ=255)
tGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACACCTGTAtt > 2:630094/1‑76 (MQ=255)
ggTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 1:2008035/1‑76 (MQ=255)
ggTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 1:739679/1‑76 (MQ=255)
gTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:5637192/76‑1 (MQ=255)
gTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:3668411/76‑1 (MQ=255)
cTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 2:202673/76‑1 (MQ=255)
cTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 1:2475740/76‑1 (MQ=255)
tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 1:2158861/1‑76 (MQ=255)
tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 2:4441980/1‑76 (MQ=255)
tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 1:8155828/76‑1 (MQ=255)
tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 2:4536122/76‑1 (MQ=255)
tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 2:3400581/1‑76 (MQ=255)
gCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 2:536845/76‑1 (MQ=255)
cTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc > 1:5424106/1‑76 (MQ=255)
tGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt < 1:6547197/76‑1 (MQ=255)
gccG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 1:6436506/74‑1 (MQ=255)
gTCG‑GAAATCAGCCCCAATAACGGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 2:734228/1‑76 (MQ=255)
tCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt > 1:2524471/1‑76 (MQ=255)
cG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 2:5230574/1‑76 (MQ=255)
g‑gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:8139048/76‑1 (MQ=255)
g‑gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 1:6717087/1‑76 (MQ=255)
g‑gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:4231930/76‑1 (MQ=255)
gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:7842139/76‑1 (MQ=255)
gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:4244873/76‑1 (MQ=255)
gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:3402143/76‑1 (MQ=255)
aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:6300797/1‑76 (MQ=255)
aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:3630707/1‑76 (MQ=255)
aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 1:3294872/76‑1 (MQ=255)
aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 2:5536743/76‑1 (MQ=255)
aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:81030/1‑76 (MQ=255)
aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc > 1:6103100/1‑76 (MQ=255)
aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:4239938/76‑1 (MQ=255)
aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 1:4178431/1‑76 (MQ=255)
tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa < 2:881514/76‑1 (MQ=255)
ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc > 1:5392424/1‑76 (MQ=255)
cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg > 1:1067489/1‑76 (MQ=255)
ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc < 1:2652371/76‑1 (MQ=255)
cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:2798931/1‑76 (MQ=255)
aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt < 2:7365234/76‑1 (MQ=255)
tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg > 2:4808743/1‑76 (MQ=255)
aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:2794447/1‑76 (MQ=255)
cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc < 1:7590014/76‑1 (MQ=255)
gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCTCATCGCGCAc > 2:6778178/1‑76 (MQ=255)
gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc > 1:1673653/1‑76 (MQ=255)
taaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc < 2:1133735/75‑1 (MQ=255)
cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:3701123/1‑76 (MQ=255)
cAAAACGCAAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:5219015/1‑76 (MQ=255)
aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:6537430/76‑1 (MQ=255)
aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 1:2411256/76‑1 (MQ=255)
aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:2643039/76‑1 (MQ=255)
aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:6837256/76‑1 (MQ=255)
aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg > 2:6206782/1‑76 (MQ=255)
aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 1:1444374/76‑1 (MQ=255)
aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 2:775213/76‑1 (MQ=255)
cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 2:1367455/1‑76 (MQ=255)
cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 2:1230221/1‑76 (MQ=255)
ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:1588580/1‑76 (MQ=255)
ccAACCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTCCCGATGGCGCATCGCGCACCAgccgcc < 2:489615/76‑1 (MQ=255)
cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca > 2:6863076/1‑76 (MQ=255)
cAATCCCCACCAAATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca < 1:4977025/76‑1 (MQ=255)
cAACCCCCACCAAATAAAGCATGACGCCGTTATAAAACAGGCTTTTTACCGATGGCGCATCGCGCCCCAGCCGCca < 2:2531380/76‑1 (MQ=255)
|
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
