Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F1 I1 R1
|
14 |
46.3 |
3075166 |
96.7% |
2973685 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,193,779 |
G→T |
L143I (CTT→ATT) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,193,779 | 0 | G | T | 97.7%
| 142.9
/ NA
| 43 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (19/23); minor base C (0/1); total (19/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193710‑2193851
|
aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac < 1:671484/76‑1 (MQ=255)
tGCATCGGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCGAATAACAGCAAAACGCCAATCCccacc < 1:1518247/76‑1 (MQ=255)
tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 1:1117515/76‑1 (MQ=255)
gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca < 2:457456/76‑1 (MQ=255)
aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa > 1:611440/1‑76 (MQ=255)
tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 1:1473625/76‑1 (MQ=255)
ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa > 2:1341539/1‑76 (MQ=255)
gCGTCCAGGCGCGGCGGGTCTGTCGGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:53678/76‑1 (MQ=255)
cGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAATCAGACCCAATAACAGCAAAACGCCNATCCCCACCAGATAAAGc < 1:1462705/76‑1 (MQ=255)
cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc < 1:1212046/76‑1 (MQ=255)
cGTCCAGGCGCGGCTGGGCTGGCTGTCNGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc > 1:1536958/1‑76 (MQ=255)
gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGcn < 1:150477/76‑2 (MQ=255)
gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 1:1041660/76‑1 (MQ=255)
tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:1072953/76‑1 (MQ=255)
ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:566387/76‑1 (MQ=255)
cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa > 2:1532770/1‑76 (MQ=255)
cAGGCGCGGCTGGTCTGGCTGTCGGAAACCA‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 2:949241/76‑1 (MQ=255)
ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt > 2:848020/1‑76 (MQ=255)
ggCNGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACNCCAATCCCCACCAGATAAAGCATGACGCCTGt < 2:1430804/76‑1 (MQ=255)
tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:838525/76‑1 (MQ=255)
ggTCAGGCTGTCGGAAAACAGCCCCAATAACAGCAAAACGCCAATCCCCAGCAGATAAAGCATGTCGCCGGTANTa > 2:1441108/1‑76 (MQ=255)
gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:594539/76‑1 (MQ=255)
ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 1:903300/76‑1 (MQ=255)
tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 2:644548/1‑76 (MQ=255)
gTCGGAAATCAGCNCCAATAACAGCAAAACGCCAATCCCCACCAGGTAAAGCATGACGCCTGTATTAAACAGGCtt < 2:1391510/76‑1 (MQ=255)
ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:664377/76‑1 (MQ=255)
ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:733459/76‑1 (MQ=255)
ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:1250829/76‑1 (MQ=255)
ggAAATCAGCCCCAATAACAGCAAAACGCCAACCCCCCCCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 1:728709/76‑1 (MQ=255)
gAAATCAGCTCCAATAACAGCAANACGCCNATCCCGACCAGATANAGAATGACGCCTGTATTAAACAGGCTTTTTa > 2:199628/1‑76 (MQ=255)
cAGCCCCAATAACAGCAAAAGGACAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt > 1:12284/1‑76 (MQ=255)
cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:988557/76‑1 (MQ=255)
aGCCTCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACCCCTGTATTAAACAGGCCTTTTGCCGNTg > 2:1441303/1‑76 (MQ=255)
aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg > 1:723473/1‑76 (MQ=255)
gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg > 2:906191/1‑76 (MQ=255)
gattAACAGCAAANCGCCAATCCCGACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa < 1:199628/73‑1 (MQ=255)
tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg > 2:281707/1‑76 (MQ=255)
aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:951298/1‑76 (MQ=255)
cAGCAAAACGCCAATCCCCACCAGATAAAACATGACCCCTGTATTAAACAGGCTTTTNACCGATGGCGCATCgcgc < 1:1457511/76‑1 (MQ=255)
aaaaCGCCAATCCCCACTAGATAAAGCATGACGCCTGTATTAAACAGGCTATTTACCGATGGCGAATCGCGCCNCa > 2:1444485/1‑76 (MQ=255)
aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 2:140688/1‑76 (MQ=255)
ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:1334087/1‑76 (MQ=255)
ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:431455/1‑76 (MQ=255)
ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:450559/1‑76 (MQ=255)
cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca < 1:776348/76‑1 (MQ=255)
|
ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193710‑2193851
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
