Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F71 I0 R1	83	59.3	2271602	90.4%	2053528	142.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	143.2 / ‑6.7	51	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (26/23); minor base C (2/0); total (28/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754306‑754584

aTTCGCAGGATGCGGTTGAAGTGAAGACTGGGTCAAAGGTGAACACACCGTGTGAACAGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTCCAGCACATCCAGTTCAGCAGCCGCGTCCCCGCGGAgggt‑gggt                                                                                                                                          >  2:707831/1‑143 (MQ=255)

   cGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGtttt                                                                                                                                       >  1:1013860/1‑143 (MQ=255)

         aTGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCtttt                                                                                                                                 <  1:177700/143‑1 (MQ=255)

                    gtgaggTCTGGGTTAGAGGGGAAGACACCTTTTGAACCGAGAGTACGGATATCAATAACATCGTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCAt                                                                                                                      <  2:284829/143‑1 (MQ=255)

                    gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGGGT‑GCGGTTTGCTTCTGCATCAGcccg                                                                                                                      >  1:457105/1‑141 (MQ=255)

                    gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCAt                                                                                                                      >  1:1065221/1‑143 (MQ=255)

                    gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCAt                                                                                                                      <  1:910268/143‑1 (MQ=255)

                      gaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTCGAGGTG‑GGGTTTTGCTTCTGTATCCGCCAttt                                                                                                                    >  2:561966/1‑143 (MQ=255)

                         gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                 <  1:905685/143‑1 (MQ=255)

                         accggggTAAAGGGTGGACACACCTGTGGAACCGAGAGTCCGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCCGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                 <  1:105125/139‑1 (MQ=255)

                          ccTGCGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAgg                                                                                                                <  2:375240/143‑1 (MQ=255)

                            tGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGtc                                                                                                              >  2:1087808/1‑143 (MQ=255)

                                tCAAATGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCATCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCt                                                                                                          <  2:573786/143‑1 (MQ=255)

                                  aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                        <  2:720932/143‑1 (MQ=255)

                                   aaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTATATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAg                                                                                                       >  1:920688/1‑143 (MQ=255)

                                      gTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAgcgc                                                                                                    >  1:1120689/1‑143 (MQ=255)

                                              acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                            >  1:901862/1‑143 (MQ=255)

                                                  tttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                        <  1:506074/143‑1 (MQ=255)

                                                       aCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTg                                                                                   <  1:71651/143‑1 (MQ=255)

                                                        ccGAGAGTACGGATATCAATAACATCTTGACCCACCGTGCCTTTCAGCACATCCACTCCACCAGCGATATCCCCGCCGCGCGT‑GGGTTTTCCTTCTGTACCACCCAGTTACGCCCTCCGTAGCTCCTCCTCGCGTAAGCCTGc                                                                                  >  2:338299/1‑143 (MQ=255)

                                                        ccGAGAGTACGGATATCAATAACATCTTGAAGGAGAGCAACAGCCAGCACATCAAGTTCAACAGCCGTACCCCCGCTGAGGGT‑GGGATCCCCTAAAGAATCAGCCATCTAAGGACACCTGAGCGCCCCATTGCGTAAAACtac                                                                                  >  2:400113/1‑141 (MQ=255)

                                                         cGAGAGTACTGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcc                                                                                 <  2:666409/143‑1 (MQ=255)

                                                          gagaGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCAGTTGAGGGTGGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcc                                                                                 >  2:689866/1‑143 (MQ=255)

                                                             aggaCGGATATCAATAACATCTTGACCCGGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                             <  1:285017/140‑1 (MQ=255)

                                                             agTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                             <  2:702548/143‑1 (MQ=255)

                                                             agTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATACAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGTGCCTTATTGCGTATGACTGCCgtta                                                                             >  1:119135/1‑140 (MQ=255)

                                                                       tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTACGGTCTCCTTAGCGCCTTCTTGCGTGAGACTGCCGGAACTTAAAtttt                                                                   >  2:409522/1‑142 (MQ=255)

                                                                        cAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGCACTTAAATTTGc                                                                  >  2:461527/1‑143 (MQ=255)

                                                                        cAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGAGTATGAGTGCCTGAACTTTAAtttaa                                                                  >  1:759857/1‑141 (MQ=255)

                                                                            aaCATCTTGACCCAGCGTGCCTTTCAGCACATACAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTc                                                              >  1:162753/1‑143 (MQ=255)

                                                                             aCATCTTGACGCAGCGTGCCTTGCCGCATATCCAGGTCAACAGCTGTATCCCCGGTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGGCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCg                                                             <  1:479800/143‑1 (MQ=255)

                                                                               aacTTGACCCAGCGTGCCTTTCAGCACATTCAGTTCAACATCTGTATCTCCGTTGAGTGT‑GGTTTTTGCTTTTGTATCAGCCATTTTATGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGca                                                           <  1:849376/141‑1 (MQ=255)

                                                                                tCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTGATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcac                                                          >  2:927021/1‑143 (MQ=255)

                                                                                 cTTGACCCGGCGGGCCGTTCAGCACATCCAGTTCAACAGGTGGATCCCCGTTGAGGGT‑GGGTTTTGGTTTTGTATCAGCCATTTGAGGTCGCCTTAGCGGCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcaca                                                         <  1:190753/143‑1 (MQ=255)

                                                                                      cccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGCTGAGGGT‑GCGTTTTGCTTTTGTATCAGCCATTTAAGGTGTCCTTAGCGCCTTATTGCGTAACACTGCGGGCACTGAAATTTGCGTTCGCACACCAAc                                                    >  2:243971/1‑143 (MQ=255)

                                                                                          gCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTgg                                                <  1:899394/143‑1 (MQ=255)

                                                                                                cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                          >  1:438047/1‑143 (MQ=255)

                                                                                                 tttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGC‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTCTAcc                                         >  1:1116485/1‑143 (MQ=255)

                                                                                                        acaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCAATTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttttt                                  >  2:559508/1‑143 (MQ=255)

                                                                                                            ccAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAtt                              <  1:1087808/143‑1 (MQ=255)

                                                                                                              aGTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTCCCCGTTTTTTATTTg                            >  2:57171/1‑143 (MQ=255)

                                                                                                               gTTCAACAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTgg                           <  2:392239/143‑1 (MQ=255)

                                                                                                                    aCATCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTGTGTCTCAGCCATTTAACGTCCCCTTAGCGCCTTATTGCGTAAGCCTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc                      <  1:280445/143‑1 (MQ=255)

                                                                                                                    aCAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc                      >  2:406772/1‑143 (MQ=255)

                                                                                                                    aCAGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc                      <  1:400113/143‑1 (MQ=255)

                                                                                                                      aGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                    >  1:615969/1‑143 (MQ=255)

                                                                                                                      aGCTGTATCCCCGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTACGAGCATTATCTTGGCTTTACCCGTTTTTTATTTGGCTcgccg                    >  1:766398/1‑143 (MQ=255)

                                                                                                                             tCCCCGTTGAGGGC‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgtg             <  1:1083208/143‑1 (MQ=255)

                                                                                                                               cccGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaa           >  1:925441/1‑143 (MQ=255)

                                                                                                                               cccGTTGAGGGT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaa           >  1:932233/1‑143 (MQ=255)

                                                                                                                                    gggtggt‑gggtgTTGTTTTTGTTTCAGCCATTTAAGGTCTCCTTAGCGGCTTATTGCGTAAGACTGTCGGAACGTAACTTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  2:250062/139‑1 (MQ=255)

                                                                                                                                    ggAGGGT‑GGGTTTTGCTGTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCTGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  2:287645/142‑1 (MQ=255)

                                                                                                                                        ggT‑GGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  >  1:637422/1‑143 (MQ=255)

ATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGT‑GAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754306‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A