Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F59 I1 R1	23	54.9	2144786	90.8%	1947465	141.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	150.3 / NA	52	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/25); total (27/25)

GATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754314‑754580

gATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCttt                                                                                                                              <  2:839381/143‑1 (MQ=255)

 aTGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                             <  1:755666/143‑1 (MQ=255)

    cGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACACCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTCGTa                                                                                                                          >  2:736866/1‑143 (MQ=255)

       ttGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCCACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                       >  1:111352/1‑143 (MQ=255)

       ttGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                       >  1:840928/1‑143 (MQ=255)

            gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCCGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAt                                                                                                                  <  1:556960/143‑1 (MQ=255)

            gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCACCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGGGTCGGTTTTGCTCTTGTATCAGCCAt                                                                                                                  >  2:252663/1‑143 (MQ=255)

             tgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAtt                                                                                                                 >  1:614335/1‑143 (MQ=255)

                agCCTGGGTCAAAGCTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                              <  1:993622/143‑1 (MQ=255)

                 ggcggggTCAAAGGTGAACAGACCTTTGGAGCCGAGCGTGGAAATAACAATAACATCTTGACCCAGCGTGCCGTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  1:888540/139‑1 (MQ=255)

                 gCCGGGGTCAAAGGGGAACACCCCTTTGGGACCGGGAGTACGGATATCAATAACAGCTTGACCCAGCGTGCCTGTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  2:182128/143‑1 (MQ=255)

                  ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGAGCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTGTGCTTTTGTATCAGACATTTACgg                                                                                                            >  1:277924/1‑143 (MQ=255)

                       gTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTcc                                                                                                       <  2:574261/143‑1 (MQ=255)

                         cAAAGGTGAACTCACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                     <  1:898777/143‑1 (MQ=255)

                          aaaGCTGACCACACCTTTTCAACCGCGTGTTCGGATATCACTAACAGCGTGACCCAGCGTGCCTTTCAGCACATTCAGTCCTTCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                    <  2:409644/143‑1 (MQ=255)

                              gTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTGTCGTTAgcgc                                                                                                >  1:500308/1‑143 (MQ=255)

                                   cacacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAt                                                                                           >  2:851127/1‑143 (MQ=255)

                                    acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTGAGCGCCTTAtt                                                                                          >  1:983470/1‑143 (MQ=255)

                                      tccTTTTGAACTGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGTGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                        <  1:820469/142‑1 (MQ=255)

                                      acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCGTTAGCGCCTTATTGc                                                                                        >  1:486042/1‑143 (MQ=255)

                                       ccTGTTGAAGCGAGCGTACGGATATCAATAGCATCTGTGCCCAGCGGGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTAGCAGCAAAATAAGTGTTTCTTAGCGTCTTATTGCg                                                                                       <  2:936177/143‑1 (MQ=255)

                                         ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTa                                                                                     >  2:484041/1‑143 (MQ=255)

                                         ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACACCAAGATCACAAGCTATCACCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTACTGCGTa                                                                                     >  2:377443/1‑143 (MQ=255)

                                            tGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                  <  1:926232/143‑1 (MQ=255)

                                            tGAACCGAGAGTACGGATAGCAATAACATCTTTTCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTCAGa                                                                                  <  1:556406/143‑1 (MQ=255)

                                                   agagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAGGGTCTCCTTCGCGCCTTATTGCGTAAGACTGACgg                                                                           >  2:88329/1‑143 (MQ=255)

                                                    gagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcagaa                                                                          >  1:260856/1‑139 (MQ=255)

                                                    gagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGa                                                                          <  2:496971/143‑1 (MQ=255)

                                                      gTACGGACATCAAGAACGTCTGGCCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTCTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAAc                                                                        <  1:900226/143‑1 (MQ=255)

                                                       tACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACt                                                                       >  1:848718/1‑143 (MQ=255)

                                                          ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                                    <  1:271211/143‑1 (MQ=255)

                                                          ggAGATCAAGAACGTCTTGCCCCAGCGTGCCTTTCAGCACAGCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGGATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                                    <  1:111834/143‑1 (MQ=255)

                                                                 aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGcc                                                             >  2:231371/1‑143 (MQ=255)

                                                                  aTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTACCt                                                            >  2:541384/1‑143 (MQ=255)

                                                                   tAACATCTTGACCCAGCGTGCCTTTCAGAACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAGTTGCCtg                                                           >  2:764698/1‑142 (MQ=255)

                                                                             aCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCaa                                                 >  1:221549/1‑143 (MQ=255)

                                                                                        cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                      <  1:210695/143‑1 (MQ=255)

                                                                                                 caTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt                             <  1:563962/143‑1 (MQ=255)

                                                                                                  aTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAAGTTAAATTTGCCTTCGCACATCAACCTGGCTTTACTCGTTTTTTa                            >  1:508391/1‑143 (MQ=255)

                                                                                                      aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTg                        >  1:840976/1‑143 (MQ=255)

                                                                                                          cAACAGCTGTATCCCCGTTGAGGGTTGGTTTTGCTTTTGTATCAGCCATTTATGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTc                    <  2:597238/143‑1 (MQ=255)

                                                                                                          cAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTc                    >  2:60834/1‑143 (MQ=255)

                                                                                                          cAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTGTTTATTTGGCTc                    >  1:165164/1‑143 (MQ=255)

                                                                                                           aacgcgtgtgTCCCCGTTGTGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTCTTTGGCTcg                   <  2:1026649/137‑1 (MQ=255)

                                                                                                                cTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGACCCTACGACTCGCTTTTCCCGTTTTTTATTTGGCTCGCCGct              >  1:723360/1‑143 (MQ=255)

                                                                                                                 tGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATCTAAGATCTCCTTAGCGCCTTATTGCGTAGGACTGCCGGAACTTACATTTGCCTTCGCCCATCCACCTGGCCTTGCTCCATTTTCATTTGGCTCgctgctg             >  2:873746/1‑142 (MQ=255)

                                                                                                                    agccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt          <  1:484041/141‑1 (MQ=255)

                                                                                                                     tCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCGTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGGCGCTCtgtt         >  2:563065/1‑142 (MQ=255)

                                                                                                                       cccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTAACTGGTGTTGTTTTTGACTTGCCGCTCggtcta       >  2:748076/1‑140 (MQ=255)

                                                                                                                           ttGAGGGTTGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGGAtgag   <  2:712330/143‑1 (MQ=255)

                                                                                                                           ttGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAagag   <  1:426886/143‑1 (MQ=255)

                                                                                                                            ggAGGGTGGGTCTCGCTTTTGTATCAGCCATTTAAACTCAACACATCGCCGTATAGCAAACTAGTTCCGTAACTTAAATTTGCCTTCTCACAACAACTTGGCTTGACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg  <  1:145533/142‑1 (MQ=255)

GATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754314‑754580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A